A5051860758- Blood transfusion and management
- Blood donation and transfusion practices
- Erythrocyte Function and Pathophysiology
- Blood groups and transfusion
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Hemoglobinopathies and Related Disorders
- Platelet Disorders and Treatments
- Hematopoietic Stem Cell Transplantation
- Neonatal Health and Biochemistry
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Clinical Nutrition and Gastroenterology
- Blood properties and coagulation
- Pancreatic function and diabetes
- Erythropoietin and Anemia Treatment
- Medical Imaging and Pathology Studies
- Hemoglobin structure and function
- Cannabis and Cannabinoid Research
- Hematological disorders and diagnostics
- Trauma and Emergency Care Studies
- Radiopharmaceutical Chemistry and Applications
- Oral health in cancer treatment
- Immunotoxicology and immune responses
- Neutropenia and Cancer Infections
- Iron Metabolism and Disorders
- Bacterial Identification and Susceptibility Testing
University of Cincinnati Medical Center
1994-2023
University of Cincinnati
2005-2019
Dartmouth–Hitchcock Medical Center
2006-2012
United States Army Medical Research and Development Command
2012
American Red Cross
2006-2012
Dartmouth College
2012
Versiti Blood Center of Wisconsin
2012
Terumo (United States)
2012
Fast Track Drugs and Biologics
2012
Cerus (United States)
2006-2011
BACKGROUND: Availability of platelets (PLTs) is severely limited by shelf life in some settings. Our objective was to determine and compare Food Drug Administration (FDA) criteria the PLT recovery survival autologous PLTs cryopreserved at −65°C or less 6% dimethyl sulfoxide (DMSO) reconstituted with a no‐wash method (cryopreserved [CPPs]) compared fresh PLTs. STUDY DESIGN AND METHODS: This randomized, Phase I study analyzing viability vitro function consenting healthy subjects. Apheresis...
Transfusion of long-stored red blood cells (RBCs) is associated with decreased in vivo RBC recovery, delivery breakdown products, and increased morbidity mortality. Reducing the burden this "storage lesion" a major challenge transfusion medicine. Additive solution-7 (AS-7) new storage solution designed to improve metabolism by providing phosphate increasing buffering capacity.Storage quality AS-7 was measured prospective, randomized, three-center trial using units whole from healthy human...
The short dating period of room temperature-stored platelets (PLTs; 5-7 days) limits their availability at far-forward combat facilities and remote civilian sites in the United States. PLT cryopreservation 6% DMSO storage for up to 2 years may improve timely bleeding patients.A dose escalation trial DMSO-cryopreserved PLTs (CPPs) compared standard liquid-stored (LSPs) was performed patients with thrombocytopenia. Within each four cohorts, six received escalating doses CPP (0.5 unit, 1...
BACKGROUND Blood transfusion is a lifesaving intervention for millions of recipients worldwide every year. Storing blood makes this possible but also promotes series alterations to the metabolism stored erythrocyte. It unclear whether metabolic storage lesion correlated with clinically relevant outcomes and strategies aimed at improving quality units, such as hypoxic storage, ultimately improve performance in transfused recipient. STUDY DESIGN AND METHODS Twelve healthy donor volunteers were...
BACKGROUND: A novel system using ultraviolet (UV) light and riboflavin (Mirasol System, CaridianBCT Biotechnologies) to fragment nucleic acids has been developed treat whole blood (WB), aiming at the reduction of potential pathogen load white cell inactivation. We evaluated stored red (RBC) metabolic status viability, in vitro vivo, riboflavin/UV light–treated WB (IMPROVE study). STUDY DESIGN AND METHODS: The study compared recovery survival RBCs obtained from nonleukoreduced treated three...
Whole blood (WB) is an efficient product for field medical resuscitation because of its unitary composition, tolerance storage on ice and in refrigerators, simplicity use. We measured quality parameters a novel 8-week WB system. Here, 500 mL from healthy donors was collected 70 CPDA-1, leukoreduced with platelet-sparing filter, pooled into ABO-compatible two-unit pools, split matched pairs equal volume designated as Test or Control units. units received additional 50 preservative solution...
BACKGROUND Pathogen reduction (PR) of whole blood (WB) may increase safety when applied before component separation. This study evaluates the in vivo performance red cells (RBCs) derived from WB treated with riboflavin and ultraviolet (UV) light PR (Mirasol) system. STUDY DESIGN AND METHODS was a prospective, two‐center, single‐blind, randomized, two‐period, crossover clinical trial designed to evaluate autologous 51 Cr/ 99m Tc‐radiolabeled recovery survival RBCs Mirasol‐treated compared...
Background and Objectives Pathogen reduction technology using amustaline (S‐303) was developed to reduce the risk of transfusion‐transmitted infection adverse effects residual leucocytes. In this study, viability red blood cells ( RBC s) prepared with a second‐generation process stored for 35 days evaluated in two different centres. Materials Methods single‐blind, randomized, controlled, two‐period crossover study n = 42 healthy subjects), amustaline‐treated (Test) or Control s were random...
BACKGROUND: Better storage can improve RBC availability and safety. Optimizing ATP production minimizing hemolysis has allowed progressively longer storage. STUDY DESIGN AND METHODS: In the first study, 24 units of packed CPD RBCs were pooled in groups four, realiquoted, added to 300 mL one four variants experimental additive solution 76 (EAS‐76) containing 45, 40, 35, or 30 mEq per L NaCl. Units sampled weekly for 12 weeks morphologic biochemical measures. second 10 volunteers donated 2 a...
BACKGROUND: Transfusion‐transmitted infections and immunologic effects of viable residual lymphocytes remain a concern in red blood cell (RBC) transfusion. Pathogen reduction technologies for RBC components are under development to further improve transfusion safety. S‐303 is frangible anchor‐linker‐effector with labile alkylating activity robust pathogen profile. This study characterized the viability RBCs prepared second‐generation process stored 35 days. STUDY DESIGN AND METHODS: was...
Red blood cell (RBC) storage solutions work in a narrow pH range between 7.2 and 6.4. While keeping RBC within that range, ATP production can be increased by buffering or dilution.In the first study, 12 units of packed CP2D RBCs were pooled groups four, re-aliquoted, added to one four additive (ASs): AS-3, 110 mL; EAS-61, 170 EAS-78, EAS-81, mL. EAS-78 -81 contain bicarbonate. Units sampled approximately weekly for 10 weeks biochemical measures. In second volunteers donated measures (51)Cr...
Background The haemolysis level at the end of storage is a performance parameter for RBC preparations. In evaluation new devices or processes processing blood, it relevant to evaluate whether linked (1) specific characteristics blood donor, (2) nature blood-processing methodologies. Materials and Methods As part validation automated whole system compared current manual methods, randomized, paired crossover studies were conducted evaluating measures component quality, including over 42 days...
The effect of storing packed RBCs suspended in 300 mL an alkaline, experimental additive solution (EAS 64) was explored.RBC units prepared from blood collected healthy donors into CPD were WBC reduced and stored for 10 weeks under bank conditions after the addition EAS 64 (adenine, 2 mM:; dextrose, 50 mannitol, 20 NaCl, 75 Na(2)HPO(4), 9 mM:). For comparison, non-WBC-reduced same a different (AS-1, Baxter Healthcare) 6 weeks. Standard methods used vitro assays. 24-hour vivo recoveries...
BACKGROUND: Platelets (PLTs) stored in additive solutions (PASs) may reduce the risk of several plasma‐associated adverse transfusion reactions such as allergic and potentially transfusion‐associated lung injury. The objective this study was to determine vitro characteristics vivo radiolabeled recovery survival apheresis PLTs (APs) a new PAS compare latter Food Drug Administration (FDA) criteria. STUDY DESIGN AND METHODS: Hyperconcentrated APs were collected from healthy subjects paired...
BACKGROUND: Whole blood (WB) must be refrigerated within 8 hours to optimize Factor VIII : C yield, but chilled platelets (PLTs) are rapidly removed from circulation and cannot used clinically. It is logistically preferable hold WB overnight at room temperature for next‐day processing. We compared in vitro quality of PLT‐rich plasma (PRP)‐derived PLT concentrates (PCs) fresh versus overnight‐held WB. STUDY DESIGN AND METHODS: Four units were pooled split prevent donor‐dependent differences....
Background Overnight, room temperature hold ( ONH ) of whole blood before component processing offers several benefits. This study evaluated the storage and in vivo recovery characteristics red cells RBCs stored additive solution‐7 AS ‐7). Study Design Methods We conducted a three‐center, three‐arm evaluation new collection system with ‐7 compared to leukoreduced processed within 8 hours ‐1 (control). Whole (500 ± 50 mL from healthy research subjects (n = 240) was held at 0 2 hours, 6 or...
RBC ATP concentrations are the most important correlate of viability. Tests were performed to determine whether increased AS volume, pH, and phosphate content stored concentrations.In three studies, packed RBCs pooled in groups 3 or 4 units realiquoted as combined reduce intradonor differences. Pooled licensed ASs, AS-1 AS-5, which contain saline, adenine, glucose, mannitol (SAGM), experimental ASs (EASs) containing SAGM disodium phosphate. Ten pools at equivalent 100, 200, 300 mL AS. Six...
BACKGROUND: This study explored the effect of storing packed RBCs suspended in 200 mL an alkaline, hypotonic, experimental additive solution (EAS 61). STUDY DESIGN AND METHODS: Packed RBC units prepared from collected healthy donors CPD were stored for 8 (n = 10) and 9 weeks under blood bank conditions after addition EAS 61 (adenine, 2 m M ; dextrose, 110 mannitol, 55 NaCl, 26 Na HPO 4 , 12 ). Standard methods used vitro assays. The 24‐hour vivo autologous recoveries measured with 51 Cr....
A portable automated component collection system that produces double (2) units of leukoreduced RBCs (DRBCs) from a single donation was evaluated. This study analyzed quality the collected and final products, efficacy leukoreduction, donor safety.The used to collect 120 DRBCs. WBCs were removed 90 products with machine-controlled filtration. DRBCs in ACD-A stored AS-1 for 42 days at 1 6 degrees C. Pre- postprocedure vital signs hematologic parameters measured. Procedure time, product...
BACKGROUND: A nucleic acid–targeted pathogen inactivation process with S‐303 was developed to treat red blood cells (RBCs). STUDY DESIGN AND METHODS: Three studies in healthy subjects investigated posttransfusion recovery, life span, and immunogenic potential of autologous RBCs treated stored for 35 days. two‐arm trial 42 (1A) examined recovery 35‐day‐old after a single transfusion. one‐arm study (1B) measured immune response 28 multiple transfusions RBCs. randomized, crossover (1C) 29...
Background Overnight, room temperature hold of whole blood ( WB ) before leukoreduction and component processing offers significant logistic cost advantages over processed within 8 hours. Plasma prepared from held at overnight PF24RT24WB may result in a degradation plasma coagulation protein activities compared to frozen hours collection. In this study, we intended evaluate the bioequivalence BE using new collection, leukoreduction, storage system fresh‐frozen FFP after 12 months storage....
Background Granulocyte transfusion from healthy donors is used in the treatment of patients with granulocyte function defects, or transient neutropenia and severe bacterial fungal infections resistant to maximal antimicrobial treatment. Study Design Methods This study evaluated performance safety newly developed collection protocol S pectra O ptia a prospective, multicenter, open‐label, randomized, paired crossover trial compared COBE apheresis system population 32 evaluable subjects. All...
BACKGROUND: Previously, cryopreserved red blood cell (RBC) units derived from CPD/AS‐5 whole‐blood (WB) collections have been limited to 24 hours postthaw storage (1‐6°C). STUDY DESIGN AND METHODS: Sixty‐four leukoreduced (LR) and 54 nonleukoreduced (NLR) AS‐5 (n = 118) RBC 500‐mL WB were stored for 6 days, glycerolized, frozen (−70 ± 5°C) at least 14 thawed, deglycerolized, (1‐6°C) 15 days resuspended in AS‐3, using an automated closed‐system processor (ACP 215, Haemonetics). Frozen either...
Abstract Thrombosomes are trehalose‐stabilized, freeze‐dried group O platelets with a 3‐year shelf life. They can be stockpiled, rapidly reconstituted, and infused regardless of the recipient's blood type. thus represent potential alternative platelet transfusion strategy. The present study assessed safety early signals efficacy in bleeding thrombocytopenic patients. We performed an open‐label, phase 1 single doses allogeneic at three dose levels cohorts, each consisting eight patients who...