A5003941493- Blood transfusion and management
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Blood donation and transfusion practices
- Platelet Disorders and Treatments
- Hemostasis and retained surgical items
- Antiplatelet Therapy and Cardiovascular Diseases
- Blood groups and transfusion
- Trauma and Emergency Care Studies
- Hemophilia Treatment and Research
- Heparin-Induced Thrombocytopenia and Thrombosis
- Muscle and Compartmental Disorders
- Venous Thromboembolism Diagnosis and Management
- 3D Printing in Biomedical Research
- Erythropoietin and Anemia Treatment
- Pharmacogenetics and Drug Metabolism
- Poisoning and overdose treatments
- Drug-Induced Hepatotoxicity and Protection
- Periodontal Regeneration and Treatments
United States Army Institute of Surgical Research
2018-2025
Whole blood (WB) is an efficient product for field medical resuscitation because of its unitary composition, tolerance storage on ice and in refrigerators, simplicity use. We measured quality parameters a novel 8-week WB system. Here, 500 mL from healthy donors was collected 70 CPDA-1, leukoreduced with platelet-sparing filter, pooled into ABO-compatible two-unit pools, split matched pairs equal volume designated as Test or Control units. units received additional 50 preservative solution...
Refrigeration of platelets (PLTs) in a PLT additive solution (PAS) reduces activation compared to storage plasma and preserves function for at least 15 days. Currently only two PASs are licensed by the Food Drug Administration, each use with one apheresis platform. In this study, we metabolic, functional, status PLTs collected on Trima collection system stored refrigerated Isoplate (ISO) PAS an Amicus Intersol (INT) PAS.Apheresis (n = 4-7 donors) were ISO or INT PAS. walk-in refrigerator...
Abstract Background Cold storage of platelets in plasma maintains hemostatic function and is an attractive alternative to room temperature (RTPs). We have recently shown that functional differences between cold‐stored (CSPs) RTPs after 5‐day are associated with mitochondrial respiration CSPs platelet (PLT) additive solution (PAS) can maintain for at least 15 days. Study Design Methods This study tested the hypothesis cold PAS preserves integrity by reducing PLT apoptosis. or were stored...
Platelet concentrates (PLT) can be manufactured using a combination of apheresis collection devices and suspension media (plasma or platelet additive solution (PAS)). It is unclear how quality hemostatic function differ across the current in-use manufacturing methods in United States. The objective this study was therefore to compare baseline PLT collected different platforms storage media.PLT were at two sites with identical protocols (N = 5 per site, N 10 total group) on MCS® + 9000...
BACKGROUND Current limitations of platelet shelf life to 5 days have led an increasingly greater demand for hemostatic agents with longevity. The objective this study was evaluate the function a lyophilized platelet‐derived product (thrombosome [TS]) as potential alternative fresh platelets. METHODS Platelets were collected from whole blood healthy donors. TSs reconstituted water and added various configurations reassembled (platelets, plasma, RBCs); measures included rotational...
Using platelet additive solution (PAS) to dilute fibrinogen during long-term cold storage of platelets (PLTs) decreases PLT activation and increases functional shelf life. We performed a randomized, paired study assess the in vitro quality PLTs stored T-PAS+ for up 18 days evaluated against under currently allowable conditions (5-day room temperature-stored [RTP] 3-day cold-stored [CSP]).PLTs were collected from healthy volunteers (n = 10) diluted 65% T-PAS+/35% plasma before storage....
Platelets pose the greatest transfusion-transmitted infectious risk among blood products. Refrigeration of platelets can mitigate bacterial contamination and extend platelet shelf life. Implementation pathogen reduction technologies (PRTs) at banks has become increasingly popular to protect against emerging reemerging diseases. In this study, we sought evaluate effects Intercept PRT on collected different platforms cold-stored for up 21 days in plasma additive solution (PAS).Double-dose...
The United States Army has shifted doctrine to focus on large-scale combat operations against peer near-peer adversaries. Future conflicts could result in a limited supply chain, leaving medical providers with only expired blood products for treatment of hemorrhagic shock. This study evaluated quality, function, and safety metrics whole stored 1 week past regulated expiration (i.e., 35 days, CPDA-1). Whole units (n = 6) were collected citrate phosphate dextrose adenine-1 (CPDA-1)...
BACKGROUND Cold‐stored platelets are an attractive option for treatment of actively bleeding patients due to a reduced risk septic complications and preserved hemostatic function compared conventional room temperature–stored platelets. However, refrigeration causes increased platelet activation aggregate formation. Specialized pro‐resolving mediators (SPMs), cell signaling biosynthesized from essential fatty acids, have been shown modulate activation. In this study, we sought determine if...
Abstract Background Platelet concentrates (PCs) used for transfusion can be produced by apheresis or derived from whole blood (WB). The Reveos device is the first US Food and Drug Administration‐approved automated processing system that produce PCs. In this work, we evaluated quality function of Reveos‐collected PCs stored 7 days at room temperature. Study Design Methods WB was collected healthy donors componentized on day collection (Fresh) after an overnight hold (Overnight). were ( n =...
Abstract Background Thousands of units whole blood (WB) and components are transfused daily to treat trauma patients. Improved methods for storage critical support trauma‐related care. The Hemanext ONE® system offers a unique method hypoxic WB, with successfully demonstrated clinically viable RBCs. This work evaluated the focusing on platelet health function. Study Design Methods WB was collected from healthy donors processed through system. Hemoglobin oxygen saturation (HbSO 2 ) levels were...
BACKGROUND Viscoelastic tests (VETs) are used widely to monitor hemostasis in settings such as cardiac surgery. There has also been renewed interest cold stored platelets (CSPs) manage bleeding this setting. CSPs reported have altered hemostatic properties compared room temperature (RTPs), including activation of GPIIb/IIIa. We investigated whether the functional differences between CSP and RTP affected performance PlateletMapping VET on TEG 5000 6s analyzer. METHOD Platelet concentrates...
Abstract Background Whole blood (WB) reigns superior to component therapy for the treatment of hemorrhagic shock on battlefield. Though cold storage WB offers a shelf life 21 35 days, lesions and potential wastage remain. Storing in an additive solution (AS) containing apoptotic inhibitors may help preserve cell viability improve quality over extended storage. Study Design Methods Non‐leukoreduced was obtained from healthy individuals dosed with: AS, AS+Necrostatin‐1 (AS+N1), AS+Boc‐D‐fmk...
Abstract Background Acetaminophen (APAP) is a widely self‐prescribed analgesic for mild to moderate pain, but overdose or repeat doses can lead liver injury and death. Kalyra Pharmaceuticals has developed novel APAP analog, KP‐1199, currently in Phase 1 clinical studies, which lacks hepatotoxicity. In this study, the authors evaluated antinociceptive effect of KP‐1199 on thermal injury‐induced nociceptive behaviors as well hemostatic parameters using human blood samples. Methods...
Platelets stored at room temperature (22-24°C) for transfusion purposes have a shelf life of 5-7 days, or 72 h when refrigerated (1-6°C). The limited platelet products severely compromises inventory. We hypothesized that cold storage platelets in 100% plasma using xenon gas under high pressure would extend to 14 days.Double apheresis units were collected and split equally between two bags. One unit was placed hyperbaric chamber, pressurized 4 bars with xenon/oxygen mixture, refrigerator days...