A5042831521- Erythrocyte Function and Pathophysiology
- Blood transfusion and management
- Blood groups and transfusion
- Blood donation and transfusion practices
- Hemoglobinopathies and Related Disorders
- Advanced Proteomics Techniques and Applications
- Glycosylation and Glycoproteins Research
- Blood properties and coagulation
- Organ Donation and Transplantation
- Platelet Disorders and Treatments
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Monoclonal and Polyclonal Antibodies Research
- Health, Medicine and Society
- Iron Metabolism and Disorders
- Hemoglobin structure and function
- Erythropoietin and Anemia Treatment
- Healthcare Systems and Practices
- Parvovirus B19 Infection Studies
- Immunodeficiency and Autoimmune Disorders
- Chronic Lymphocytic Leukemia Research
- Hematopoietic Stem Cell Transplantation
- Blood disorders and treatments
- Metabolomics and Mass Spectrometry Studies
- T-cell and B-cell Immunology
- Neonatal Health and Biochemistry
Service Régional Vaudois de Transfusion Sanguine
2012-2023
University of Lausanne
1984-2022
Swiss Red Cross
2003-2020
Laboratoire Chrono-Environnement
2009-2017
Immungenetics (Germany)
2017
Labormedizinische Zentrum Dr Risch (Switzerland)
2015
Oregon Health & Science University
2012
University Hospital of Lausanne
1982-2009
Laboratoire Charles Fabry
2001
Centre National de la Recherche Scientifique
1995-2001
Microparticles (MPs) are small phospholipid vesicles of less than 1 microm, shed in blood flow by various cell types. These MPs involved several biological processes and diseases. have also been detected products; however, their role transfused patients is unknown. The purpose this study was to characterize those bank conditions.Qualitative quantitative experiments using cytometry or proteomic techniques were performed on derived from erythrocytes concentrates. In order count MPs, they...
Pathogen reduction of platelets (PRT-PLTs) using riboflavin and ultraviolet light treatment has undergone Phase 1 2 studies examining efficacy safety. This randomized controlled clinical trial (RCT) assessed the safety PRT-PLTs 1-hour corrected count increment (CCI(1hour) ) as primary outcome.A noninferiority RCT was performed where patients with chemotherapy-induced thrombocytopenia (six centers) were randomly allocated to receive (Mirasol PRT, CaridianBCT Biotechnologies) or reference...
Background Red blood cell–derived microparticles ( RMP s) are small phospholipid vesicles shed from RBCs in units, where they accumulate during storage. Because bioactive, it could be suggested that s mediators of posttransfusion complications or, on the contrary, constitute a potential hemostatic agent. Study Design and Methods This study was performed to establish impact coagulation isolated units. Using calibrated automated thrombography, we investigated whether affect thrombin generation...
Abstract The reference plasma protein map, obtained with immobilized pH gradients in the first dimension of two‐dimensional electrophoresis, is presented. By microsequencing, more than 40 polypeptide chains were identified. new polypeptides and previously known proteins are listed a table labeled on thus providing an update human gel database.
This publication establishes a reference human liver protein map obtained with immobilized pH gradients. By microsequencing, 57 spots or 42 polypeptide chains were identified. comparison and matching (liver, red blood cell plasma sample maps), 8 additional proteins The new polypeptides previously known are listed in table and/or labeled on the map, thus providing two-dimensional gel database. can be used to identify other samples such as rectal cancer biopsies.
Abstract This publication updates the reference plasma and red blood cell protein maps obtained with immobilized pH gradients. Seventeen polypeptide spots or chains were partially characterized by direct N ‐terminal sequencing of peptides from enzymatic digestion. Additional new polypeptides previously known proteins are listed in a table and/or labeled on maps, thus providing 1993 update human two‐dimensional gel SWISS‐2DPAGE database. SWISS‐PROT sequence databases closely linked together...
Microparticles are small phospholipid vesicles of less than 1 µm released into the blood flow by various types cells such as endothelial, platelet, white or red cells. They involved in many biological and physiological processes including hemostasis. In addition, an elevated number microparticles is observed pathological situations. context transfusion, erythrocyte-derived found cell concentrates. Their role not elucidated, they considered a type storage lesion. The purpose this review to...
Red blood cells collected in citrate-phosphate-dextrose can be stored for up to 42 days at 4 °C saline-adenine-glucose-mannitol additive solution. During this controlled, but nevertheless artificial, ex vivo ageing, red accumulate lesions that reversible or irreversible upon transfusion. The aim of the present study is follow several parameters reflecting cell metabolism, antioxidant defences, morphology and membrane dynamics during storage.Five erythrocyte concentrates were followed weekly...
This paper presents the recently introduced Off-Gel electrophoresis (OGE) technology as a versatile tool to reproducibly fractionate intact proteins and peptides into discrete liquid fractions. The coupling of two stages OGE, i.e., separation in first-stage followed by fractionation derived from each protein fraction after proteolysis second stage, results an array 15 x fractions that are directly amenable additional peptide like reverse-phase chromatography (RPC). analysis all second-stage...
In obstetrics, premature rupture of the membranes (PROM) is a frequent observation which responsible for many deliveries. PROM also associated with an increased risk fetal and maternal infections. Early diagnosis mandatory in order to decrease such complications. Despite that current biological tests allowing are both sensitive specific, contamination samples by blood can induce false positive results. Therefore, identify new potential markers (present only amniotic blood, absent blood),...
The protein heterogeneity of fractions isolated by immunoanity chromatography on anti-apolipoprotein A-I and A-I1 affinity columns was analyzed high resolution two-dimensional gel electrophoresis.The electrophoresis profiles the were automatically compared computer system MELANIE.Fractions containing apolipoproteins + only
This paper presents a comparative proteomic analysis of human maternal plasma and amniotic fluid (AF) samples from the same patient at term pregnancy in order to find specific AF proteins as markers premature rupture membranes, complication frequently observed during pregnancy. Maternal corresponding were immunodepleted remove six most abundant before systematic their protein composition. The then fractionated by IEF Off-Gel electrophoresis (OGE), digested analyzed with nano-LC-MS/MS...
Two different plasmatic plasminogen activators (PA) can be demonstrated after sodium dodecyl sulfate polyacrylamide gel electrophoresis of plasma freshly collected from resting volunteers, followed by transfer the gels onto plasminogen-rich fibrin-agarose plates. These two PA are also present in plasmas deficient coagulation Factor XI, XII, prekallikrein, or high molecular weight-kininogen. The slower-moving has an apparent 85,000 Mr and is immunologically unrelated to urokinase (UK). faster...
Iron deficiency without anemia is related to adverse symptoms that can be relieved by supplementation. Since a blood donation induce such an iron deficiency, we investigated the clinical impact of treatment after donation. One week donation, randomly assigned 154 female donors with anemia, aged below 50 years, four-week oral ferrous sulfate versus placebo. The main outcome was change in level fatigue before and intervention. Aerobic capacity, mood disorder, quality life, compliance events...
Phospholipid vesicles of less than 1 μm are present in blood physiological state and their concentration may vary under pathological conditions. Various names such as exosomes (EXS) microparticles (MPS) have been used to designate these extracellular (EVS). Although EXs MPS possibly arise from separate mechanisms, they share numerous similarities representing a challenge for purification characterization. These generally originate various types cells red cells, platelets, leukocytes or...