A5049875603- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Heat shock proteins research
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Metabolomics and Mass Spectrometry Studies
- Antifungal resistance and susceptibility
- Endoplasmic Reticulum Stress and Disease
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Genetics, Aging, and Longevity in Model Organisms
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Erythrocyte Function and Pathophysiology
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
- Hepatitis C virus research
- Nail Diseases and Treatments
- interferon and immune responses
- Identification and Quantification in Food
- Trypanosoma species research and implications
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Peptidase Inhibition and Analysis
- Fungal Biology and Applications
- CRISPR and Genetic Engineering
University of Lausanne
2016-2025
SIB Swiss Institute of Bioinformatics
2010-2014
Joint Center for Structural Genomics
2014
Laboratoire Chrono-Environnement
2012
Swiss Integrative Center for Human Health
2011
University Hospital of Lausanne
2003-2010
École Polytechnique Fédérale de Lausanne
1992-2009
University of Zurich
2005
ETH Zurich
1994-2000
University of Padua
2000
Significance Circadian clocks orchestrate daily rhythms in behavior and physiology using temporal regulation of gene expression to control core clock genes rhythmic output programs. Although transcription was shown drive extensive diurnal mRNA rhythms, less is known about the proteins. Here, we provide a proteome-wide study protein accumulation mouse liver, showing that proteins preferentially accumulate morning during night. About one-half these could not be explained by mRNAs, suggesting...
Diurnal oscillations of gene expression controlled by the circadian clock and its connected feeding rhythm enable organisms to coordinate their physiologies with daily environmental cycles. While available techniques yielded crucial insights into regulation at transcriptional level, much less is known about temporally functions within nucleus protein level. Here, we quantified temporal nuclear accumulation proteins phosphoproteins from mouse liver SILAC proteomics. We identified around 5,000...
We describe a method for comparative quantitation and de novo peptide sequencing of proteins separated either by standard chromatographic methods or one- two-dimensional polyacrylamide gel electrophoresis. The approach is based on the use an isotopically labeled reagent to quantitate (by mass spectrometry) ratio peptides from digests protein being expressed under different conditions. allows changes occurring in spots bands that contain more than one has greater dynamic range most staining...
Significance Inflammasomes are cellular sensors of harmful situations such as the presence microbes or alterations in homeostasis. Upon activation, inflammasomes trigger proteolytic maturation and release inflammatory cytokines to initiate immune repair responses. The assembly inflammasome relies on a diverse repertoire sensor proteins that can detect specific stimuli. For example, AIM2 is activated by microbial DNA within cytosol. In this paper, nuclear envelope integrity found cause...
Abstract Hop/Stip1/Sti1 is thought to be essential as a co-chaperone facilitate substrate transfer between the Hsp70 and Hsp90 molecular chaperones. Despite this proposed key function for protein folding maturation, it not in number of eukaryotes bacteria lack an ortholog. We set out identify characterize its eukaryote-specific function. Human cell lines budding yeast with deletions Hop/Sti1 gene display reduced proteasome activity due inefficient capping core particle regulatory particles....
Summary Aspergillus fumigatus secretes a serine alkaline protease (ALP) and metalloprotease (MEP) when the fungus is cultivated in presence of collagen as sole nitrogen carbon source. The gene encoding ALP was isolated characterized previously. We report here cloning sequencing MEP. Genomic cDNA clones were from A. libraries using synthetic oligonucleotides probes. Stretches deduced amino acid sequence found to be agreement with N ‐terminal MEP internal peptide sequences. enzyme contains...
Images obtained from high-throughput mass spectrometry (MS) contain information that remains hidden when looking at a single spectrum time. Image processing of liquid chromatography-MS datasets can be extremely useful for quality control, experimental monitoring and knowledge extraction. The importance imaging in differential analysis proteomic experiments has already been established through two-dimensional gels now foreseen with MS images. We present MSight, new software designed to...
Proteins can be identified using a set of peptide fragment weights produced by specific digestion to search protein database in which sequences have been replaced calculated for various cleavage methods. We present method multidimensional searches that greatly increases the confidence level identification, allowing DNA sequence databases examined. This provides link between 2-dimensional gel electrophoresis and genome sequencing projects. Moreover, increased allows unknown proteins matched...
Cul3 (Cullin3)-based E3 ubiquitin ligases recently emerged as critical regulators of mitosis. In this study, we identify two mammalian BTB (Bric-a-brac–Tramtrack–Broad complex)-Kelch proteins, KLHL21 and KLHL22, that interact with are required for efficient chromosome alignment. Interestingly, but not KLHL22 is necessary cytokinesis regulates translocation the chromosomal passenger complex (CPC) from chromosomes to spindle midzone in anaphase, similar previously described BTB-Kelch proteins...
Classic semiquantitative proteomic methods have shown that all organisms respond to a mild heat shock by an apparent massive accumulation of small set proteins, named heat-shock proteins (HSPs) and concomitant slowing down in the synthesis other proteins. Yet unexplained, increased levels HSP messenger RNAs (mRNAs) may exceed 100 times ensuing relative We used here high-throughput quantitative proteomics targeted mRNA quantification estimate human cell cultures mass copy numbers most...
Circadian rhythmicity in renal function suggests rhythmic adaptations metabolism. To decipher the role of circadian clock metabolism, we studied diurnal changes metabolic pathways using integrated transcriptomic, proteomic, and metabolomic analysis performed on control mice with an inducible deletion regulator Bmal1 tubule (cKOt). With this unique resource, demonstrated that approximately 30% RNAs, 20% proteins, metabolites are kidneys mice. Several key pathways, including NAD+ biosynthesis,...
Dermatophytes are human and animal pathogenic fungi which cause cutaneous infections grow exclusively in the stratum corneum, nails hair. In a culture medium containing soy proteins as sole nitrogen source substantial proteolytic activity was secreted by Trichophyton rubrum , mentagrophytes Microsporum canis . This 55–75 % inhibited o -phenanthroline, attesting that metalloproteases were all three species. Using consensus probe constructed on previously characterized genes encoding ( MEP )...
In obstetrics, premature rupture of the membranes (PROM) is a frequent observation which responsible for many deliveries. PROM also associated with an increased risk fetal and maternal infections. Early diagnosis mandatory in order to decrease such complications. Despite that current biological tests allowing are both sensitive specific, contamination samples by blood can induce false positive results. Therefore, identify new potential markers (present only amniotic blood, absent blood),...
To investigate the ability of Escherichia coli K12 MG1655 to cope with excess and limitation a carbon energy source, we studied changes in kinetic properties two‐dimensional (2D) gel protein patterns an E. culture. The population was transferred from glucose‐excess batch glucose‐limited continuous culture (D = 0.3 h −1 ), which it cultivated for 500 (217 generations) then back Two different stages glucose‐limitation were recognized: short‐term physiological adaptation characterized by...