A5077227657- Nail Diseases and Treatments
- Antifungal resistance and susceptibility
- Plant Pathogens and Fungal Diseases
- Fungal Infections and Studies
- Peptidase Inhibition and Analysis
- Fungal Biology and Applications
- Enzyme Production and Characterization
- Mycotoxins in Agriculture and Food
- Fungal and yeast genetics research
- Infectious Diseases and Mycology
- Mycorrhizal Fungi and Plant Interactions
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Essential Oils and Antimicrobial Activity
- Biochemical and Structural Characterization
- Probiotics and Fermented Foods
- HIV/AIDS oral health manifestations
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Biofuel production and bioconversion
- Studies on Chitinases and Chitosanases
- Neuropeptides and Animal Physiology
- Protease and Inhibitor Mechanisms
- Plant-Microbe Interactions and Immunity
- Plant and fungal interactions
University Hospital of Lausanne
2016-2025
University of Lausanne
2012-2025
Southwest University of Visual Arts
2023
Hôpital Orthopédique de la Suisse Romande
1989-2017
State Key Laboratory of Mycology
2014
Centre National de la Recherche Scientifique
2014
Hôpital de Beaumont
1996-2013
Laboratoire Chrono-Environnement
2012
University of Liège
2003
University of Tübingen
2002
Azole antifungal agents, and especially fluconazole, have been used widely to treat oropharyngeal candidiasis in patients with AIDS. An increasing number of cases clinical resistance against often correlating vitro resistance, reported. To investigate the mechanisms toward azole agents at molecular level C. albicans isolates, we focused on related cellular target azoles, i.e., cytochrome P450(14DM) (14DM) those regulating transport or accumulation fluconazole. The analysis sequential...
The authors determined the role of Candida colonization in development subsequent infection critically ill patients.A 6-month prospective cohort study was given to patients admitted surgical and neonatal intensive care units a 1600-bed university medical center.Patients having predetermined criteria for significant revealed by routine microbiologic surveillance cultures at different body sites were eligible study. Risk factors recorded. A index daily as ratio number distinct (dbs) colonized...
Type and reference strains of members the onygenalean family Arthrodermataceae have been sequenced for rDNA ITS partial LSU, ribosomal 60S protein, fragments β-tubulin translation elongation factor 3. The resulting phylogenetic trees showed a large degree correspondence, topologies matched those earlier published phylogenies demonstrating that representation dermatophytes dermatophyte-like fungi has reached an acceptable level stability. All Trichophyton to be polyphyletic. In present paper,...
Resistance to azole antifungal agents in Candida albicans can be mediated by multidrug efflux transporters. In a previous study, we identified at least two such transporters, Cdr1p and Benp, which belong the class of A TP- b inding c assette (ABC) transporters major facilitators, respectively. To isolate additional factors potentially responsible for resistance C. albicans, hypersusceptibility Saccharomyces cerevisiae transporter mutant, δ pdr5 , these was complemented with genomic library....
Some Candida albicans isolates from AIDS patients with oropharyngeal candidiasis are becoming resistant to the azole antifungal agent fluconazole after prolonged treatment this compound. Most of C. fail accumulate agent, and has been considered a cause resistance. This phenomenon was shown be linked an increase in amounts mRNA ABC (ATP-binding cassette) transporter gene called CDR1 conferring benomyl resistance (BENr), product which belongs class major facilitator multidrug efflux...
Summary The opportunistic fungal pathogen Candida albicans produces secretory aspartyl proteinases, which are believed to be virulence factors in infection. We have studied the vitro expression of seven known members SAP gene family a range strains and serotypes by Northern analysis. SAP1 SAP3 were regulated during phenotypic switching between white opaque forms organism. SAP2 mRNA, was dominant transcript yeast form, found autoinduced peptide products Sap2 activity repressed amino acids....
Terbinafine is one of the allylamine antifungal agents whose target squalene epoxidase (SQLE). This agent has been extensively used in therapy dermatophyte infections. The incidence patients with tinea pedis or unguium tolerant to terbinafine treatment prompted us screen resistance all Trichophyton clinical isolates from laboratory Centre Hospitalier Universitaire Vaudois collected over a 3-year period and identify their mechanism resistance. Among 2,056 tested isolates, 17 (≈1%) showed...
ABSTRACT The major cause of athlete’s foot is Trichophyton rubrum , a dermatophyte or fungal pathogen human skin. To facilitate molecular analyses the dermatophytes, we sequenced T. and four related species, tonsurans equinum Microsporum canis gypseum . These species differ in host range, mating, disease progression. genomes are highly colinear yet contain gene family expansions not found other human-associated fungi. Dermatophyte enriched for families containing LysM domain, which binds...
Abstract Background An alarming increase in recalcitrant dermatophytosis has been witnessed India over the past decade. Drug resistance may play a major role this scenario. Objectives The aim of present study was to determine prevalence vitro terbinafine, itraconazole and voriconazole dermatophytes, identify underlying mutations fungal squalene epoxidase ( SQLE ) gene. Patients/Methods We analysed skin samples from 402 patients originating eight locations India. Fungi were identified by...
Scientific communication is facilitated by a data-driven, scientifically sound taxonomy that considers the end-user's needs and established successful practice. In 2013, Fusarium community voiced near unanimous support for concept of represented clade comprising all agriculturally clinically important species, including F. solani species complex (FSSC). Subsequently, this was challenged in 2015 one research group who proposed dividing genus into seven genera, FSSC described as members...
A novel 1,3-β-glucanosyltransferase isolated from the cell wall of <i>Aspergillus fumigatus</i> was recently characterized. This enzyme splits internally a 1,3-β-glucan molecule and transfers newly generated reducing end to non-reducing another forming 1,3-β linkage, resulting in elongation chains. The <i>GEL1</i> gene encoding this cloned sequenced. predicted amino acid sequence Gel1p homologous several yeast protein families encoded by <i>GAS</i> <i>Saccharomyces cerevisiae</i>,<i>PHR</i>...
Abstract A simple bioautographic agar overlay assay using Candida albicans as the indicator organism for detection and activity‐guided fractionation of antifungal compounds by thin layer chromatography has been developed. Inhibition fungal growth was assessed dehydrogenase activity with thiazolyl blue (methylthiazolyltetrazolium chloride; MTT). series clinically used antimycotic agents were tested in order to determine sensitivity assay. The compatibility technique chemically modified silica...
Secreted aspartyl proteinases (Saps), encoded by a gene family with at least nine members (SAP1 to SAP9), are one of the most discussed virulence factors produced human pathogen Candida albicans. In order study role each Sap isoenzyme in pathogenicity, we have constructed strains which harbor mutations selected SAP genes. SAP1, SAP2, and SAP3, regulated differentially vitro, were mutated targeted disruption. The growth rates all homozygous null mutants similar those isogenic wild-type...
Secreted aspartyl proteinases (Saps) from Candida albicans are encoded by a multigene family with at least nine members (SAP1 to SAP9) and considered putative virulence factors important for the pathogenicity of this human pathogen. The role Sap isoenzymes in C. has not yet been clearly established, therefore, using recent progress genetics yeast, we have constructed panel isogenic yeasts, each disruption one or several SAP genes. We focused on construction strain which three related genes...
Intracellular and secreted proteases fulfill multiple functions in microorganisms. In pathogenic microorganisms extracellular may be adapted to interactions with host cells. Here we describe two cell surface-associated aspartic proteases, Sap9 Sap10, which have structural similarities yapsins of Saccharomyces cerevisiae are produced by the human yeast Candida albicans. Sap10 glycosylphosphatidylinositol-anchored located membrane or wall. Both glycosylated, cleave at dibasic basic processing...
Summary The secreted aspartic proteinases (SAP) of Candida sp. are presumed to be potential virulence factors. In the opportunistic pathogen albicans proteinase genes identified date, SAP1, SAP2, SAP3 and SAP4 , constitute a multigene family. Before addressing possible role each in virulence, we sought isolate all members this family by screening genomic library with SAP1 probe for additional C. SAP using low‐stringency hybridization conditions. Three putative new members, SAP5, SAP6 SAP7...
Conidia of Aspergillus fumigatus adhere in vitro to host proteins and cells via the outer cell wall layer. The rodA gene A. was cloned by homology with nidulans, which is involved structure rodlets characteristic surface RODA protein sequence has 85% similarity that nidulans RODA; codes for a hydrophobin, low-molecular-weight moderately hydrophobic rich cysteines. disrupted hygromycin B resistance gene. By transformation protoplasts gene, RodA- mutants were generated. These are deficient...
Thirty human immunodeficiency virus-positive patients carrying Candida albicans in their oropharynx were treated with fluconazole and monitored for 90 to 570 days. Fluconazole-resistant C. (MIC, > 32 micrograms/ml) appeared only seven after days of treatment corresponding a total dose more than 10 g. Resistance was not associated resistance other azole derivatives. Susceptible resistant strains from each patient had the same genotype (as defined by electrophoretic karyotype restriction...
Abstract Background Millions of humans and animals suffer from superficial infections caused by a group highly specialized filamentous fungi, the dermatophytes, which exclusively infect keratinized host structures. To provide broad insights into molecular basis pathogenicity-associated traits, we report first genome sequences two closely phylogenetically related Arthroderma benhamiae Trichophyton verrucosum , both induce inflammatory in humans. Results 97% 22.5 megabase A. T. are...
We initiated a study of pIM13, multicopy, macrolide-lincosamide-streptogramin B (MLS) plasmid first isolated from strain Bacillus subtilis and described by Mahler Halvorson (J. Gen. Microbiol. 120:259-263, 1980). The copy number this was about 200 in B. 30 Staphylococcus aureus. MLS resistance determinant pIM13 shown to be highly homologous ermC, an inducible element on the S. aureus pE194. product similar size that ermC immunologically cross-reactive with it. expressed constitutively....
Chronic recalcitrant dermatophytoses, due to Trichophyton (T.) mentagrophytes Type VIII are on the rise in India and noteworthy for their predominance. It would not be wrong assume that travel migration responsible spread of T. from India, with many strains resistant terbinafine, other parts world. From September 2016 until March 2020, a total 29 (India) were isolated. All patients residents Germany: 12 females, 15 males gender remaining two was assignable. Patients originated (11), Pakistan...