A5016466390- Genetics, Aging, and Longevity in Model Organisms
- MicroRNA in disease regulation
- Sarcoma Diagnosis and Treatment
- RNA Research and Splicing
- Evolution and Paleontology Studies
- Epigenetics and DNA Methylation
- Testicular diseases and treatments
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Urologic and reproductive health conditions
- Advanced biosensing and bioanalysis techniques
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Chromosomal and Genetic Variations
- Mitochondrial Function and Pathology
- Fish Biology and Ecology Studies
- Fish biology, ecology, and behavior
- Renal and related cancers
- Plant Virus Research Studies
- Protist diversity and phylogeny
- Genetic Syndromes and Imprinting
- Congenital heart defects research
- Telomeres, Telomerase, and Senescence
- RNA modifications and cancer
- Bacteriophages and microbial interactions
Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)
2016-2025
Institute of Aging
2022
Friedrich Schiller University Jena
2021
Medical University of Białystok
2021
Institute of Medical Microbiology and Hygiene
2016
King's College London
2016
Centro Cardiologico Monzino
2016
Institute of Experimental Cardiology
2016
Imperial College London
2016
University of Florence
2016
Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated ordered physical, genetic functional sequence resource that describes barley gene-space in structured whole-genome context. We developed physical map 4.98 Gb, more than 3.90 Gb anchored to high-resolution map. Projecting deep shotgun assembly, complementary DNA RNA data onto this framework supports...
Cereal grasses of the Triticeae tribe have been major food source in temperate regions since dawn agriculture. Their large genomes are characterized by a high content repetitive elements and pericentromeric that virtually devoid meiotic recombination. Here we present high-quality reference genome assembly for barley (Hordeum vulgare L.). We use chromosome conformation capture mapping to derive linear order sequences across space investigate spatial organization chromatin nucleus at megabase...
Coccolithophores have influenced the global climate for over 200 million years. These marine phytoplankton can account 20 per cent of total carbon fixation in some systems. They form blooms that occupy hundreds thousands square kilometres and are distinguished by their elegantly sculpted calcium carbonate exoskeletons (coccoliths), rendering them visible from space. Although coccolithophores export organic matter calcite to sea floor, they also release CO2 calcification process. Hence, a...
Abstract D -Glucosamine (GlcN) is a freely available and commonly used dietary supplement potentially promoting cartilage health in humans, which also acts as an inhibitor of glycolysis. Here we show that GlcN, independent the hexosamine pathway, extends Caenorhabditis elegans life span by impairing glucose metabolism activates AMP-activated protein kinase (AMPK/AAK-2) increases mitochondrial biogenesis. Consistent with concept mitohormesis, GlcN promotes increased formation reactive oxygen...
Sepsis-associated encephalopathy (SAE) is a severe and frequent complication of sepsis causing delirium, coma, long-term cognitive dysfunction. We identified microglia C1q complement activation in hippocampal autopsy tissue patients with increased C1q-mediated synaptic pruning murine polymicrobial model. Unbiased transcriptomics isolated derived from septic mice revealed an involvement the innate immune system, activation, up-regulation lysosomal pathways during SAE parallel to neuronal...
Abstract Background Millions of humans and animals suffer from superficial infections caused by a group highly specialized filamentous fungi, the dermatophytes, which exclusively infect keratinized host structures. To provide broad insights into molecular basis pathogenicity-associated traits, we report first genome sequences two closely phylogenetically related Arthroderma benhamiae Trichophyton verrucosum , both induce inflammatory in humans. Results 97% 22.5 megabase A. T. are...
Glyoxalases (Glo1 and Glo2) are involved in the glycolytic pathway by detoxifying reactive methylglyoxal (MGO) into D-lactate a two-step reaction using glutathione (GSH) as cofactor. Inhibitors of glyoxalases considered anti-inflammatory anti-carcinogenic agents. The recent finding that various polyphenols modulate Glo1 activity has prompted us to assess curcumin's potency an inhibitor.Cultures whole blood cells tumor cell lines (PC-3, JIM-1, MDA-MD 231 1321N1) were set up investigate effect...
Differential DNA methylation is an essential epigenetic signal for gene regulation, development, and disease processes. We mapped patterns of 190 promoter regions on chromosome 21 using bisulfite conversion subclone sequencing in five human cell types. A total 28,626 subclones were sequenced at high accuracy (long-read) Sanger resulting the measurement state 580427 CpG sites. Our results show that average levels are distributed bimodally with enrichment highly methylated unmethylated...
Dietary restriction (DR) extends lifespan and promotes metabolic health in evolutionary distinct species. DR is widely believed to promote longevity by causing an energy deficit leading increased mitochondrial respiration. We here show that inhibitors of complex I physical activity, stress resistance as well Caenorhabditis elegans despite normal food uptake, i.e. the absence DR. However, inhibition does not further extend dietarily restricted nematodes, indicating impaired activity mimics...
Summary The brains of teleost fish show extensive adult neurogenesis and neuronal regeneration. patterns gene regulation during brain aging are unknown. short‐lived Nothobranchius furzeri shows markers including reduced learning performances, gliosis, neurogenesis. We used RNA ‐seq to quantify genome‐wide transcript sampled five different time points characterize whole‐genome N. . Comparison with human datasets revealed conserved up‐regulation ribosome, lysosome, complement activation...
Abstract Ageing has been defined as a global decline in physiological function depending on both environmental and genetic factors. Here we identify gene transcripts that are similarly regulated during ageing nematodes, zebrafish mice. We observe the strongest extension of lifespan when impairing expression branched-chain amino acid transferase-1 ( bcat-1 ) C. elegans , which leads to excessive levels acids (BCAAs). further show BCAAs reduce LET-363/mTOR-dependent neuro-endocrine signal,...
Abstract Barley ( Hordeum vulgare L.) is a cereal grass mainly used as animal fodder and raw material for the malting industry. The map-based reference genome sequence of barley cv. ‘Morex’ was constructed by International Genome Sequencing Consortium (IBSC) using hierarchical shotgun sequencing. Here, we report experimental computational procedures to (i) assemble more than 80,000 bacterial artificial chromosome (BAC) clones along minimum tiling path genome-wide physical map, (ii) find...
Arsenite is one of the most toxic chemical substances known and assumed to exert detrimental effects on viability even at lowest concentrations. By contrast unlike higher concentrations, we here find that exposure low-dose arsenite promotes growth cultured mammalian cells. In nematode C. elegans, resistance against thermal stressors extends lifespan this metazoan, whereas concentrations reduce longevity. While causes a transient increase in reactive oxygen species (ROS) levels co-exposure...
Article14 March 2015Open Access Source Data Smg6/Est1 licenses embryonic stem cell differentiation via nonsense-mediated mRNA decay Tangliang Li Leibniz Institute for Age Research – Fritz Lipmann (FLI), Jena, Germany Search more papers by this author Yue Shi Disease Genomics and Individualized Medicine Laboratory, Beijing of Genomics, Chinese Academy Sciences, Beijing, China Pei Wang Luis Miguel Guachalla Molecular Max-Planck-Research Department Stem Cell Aging, University Ulm, Baofa Sun...
Abstract Disease epidemiology during ageing shows a transition from cancer to degenerative chronic disorders as dominant contributors mortality in the old. Nevertheless, it has remained unclear what extent molecular signatures of reflect this phenomenon. Here we report on identification conserved transcriptomic signature based gene expression data four vertebrate species across tissues. We find that ageing-associated changes follow trajectories similar transcriptional alterations observed...
A widespread modulation of gene expression occurs in the aging brain, but little is known as to upstream drivers these changes. MicroRNAs emerged fine regulators many biological contexts and they are modulated by age. may therefore be part global correlated with aging-related phenotypes.Here, we show that microRNA-29 (miR-29) induced during short-lived turquoise killifish brain genetic antagonism its function induces a gene-expression signature typical aging. Mechanicistically, identified...
The parasitic marine dinoflagellate Amoebophrya is the first known eukaryote with aerobic mitochondria without a genome.
Cellular senescence correlates with changes in the transcriptome. To obtain a complete view on senescence-associated transcription networks and pathways, we assessed by deep RNA sequencing transcriptomes of five most commonly used laboratory strains human fibroblasts during their transition into senescence. In number cases, verified RNA-seq data real-time PCR. By determining cellular protein levels observed that age-related expression but not all genes is regulated at transcriptional level....
Skeletal muscle function crucially depends on innervation while repair of skeletal relies resident stem cells (MuSCs). However, it is poorly understood how affects MuSC properties and thereby regeneration muscle. Here, we report that loss causes precocious activation MuSCs concomitant with the expression markers myogenic differentiation. This aberrant after accompanied by profound alterations mRNA protein level. Combination injury results in impaired including shifts populations delayed...
Abstract Although DNA methylation data yields highly accurate age predictors, little is known about the dynamics of this quintessential epigenomic biomarker during lifespan. To narrow gap, we investigate trajectories male mouse colon at five different time points aging. Our study indicates existence sudden hypermethylation events specific stages life. Precisely, identify two switches early-to-midlife (3-9 months) and mid-to-late-life (15-24 transitions, separating rodents’ life into three...
Paulinella chromatophora is a cercozoan amoeba that contains "chromatophores," which are photosynthetic inclusions of cyanobacterial origin. The recent discovery chromatophores evolved independently plastids, underwent major genome reduction, and transferred at least two genes to the host nucleus has highlighted P. as model infer early steps in evolution organelles. However, owing paucity nuclear sequence data, extent endosymbiotic gene transfer (EGT) symbiont regulation currently unknown. A...