A5056935245- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Genomics and Phylogenetic Studies
- Alzheimer's disease research and treatments
- Plant-Microbe Interactions and Immunity
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Protein Structure and Dynamics
- Plant biochemistry and biosynthesis
- Mycorrhizal Fungi and Plant Interactions
- Prion Diseases and Protein Misfolding
- Computational Drug Discovery Methods
- Chemical synthesis and alkaloids
- Protein purification and stability
- Plant Pathogens and Fungal Diseases
- Microbial Metabolism and Applications
- Monoclonal and Polyclonal Antibodies Research
- Plant tissue culture and regeneration
- Viral Infectious Diseases and Gene Expression in Insects
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Microbial Community Ecology and Physiology
- Escherichia coli research studies
- Slime Mold and Myxomycetes Research
- Endoplasmic Reticulum Stress and Disease
- Vibrio bacteria research studies
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)
2014-2023
Leibniz Association
2011
Schiller International University
2008
Max Planck Institute of Biochemistry
2005
Fungi produce numerous low molecular weight molecules endowed with a multitude of biological activities. However, mining the full-genome sequences fungi indicates that their potential to secondary metabolites is greatly underestimated. Because most biosynthesis gene clusters are silent under laboratory conditions, one major challenges understand physiological conditions which these genes activated. Thus, we cocultivated important model fungus Aspergillus nidulans collection 58 soil-dwelling...
Microorganisms form diverse multispecies communities in various ecosystems. The high abundance of fungal and bacterial species these consortia results specific communication between the microorganisms. A key role this is played by secondary metabolites (SMs), which are also called natural products. Recently, it was shown that interspecies 'talk' microorganisms represents a physiological trigger to activate silent gene clusters leading formation novel SMs involved species. This review focuses...
Sequence analyses of fungal genomes have revealed that the potential fungi to produce secondary metabolites is greatly underestimated. In fact, most gene clusters coding for biosynthesis antibiotics, toxins, or pigments are silent under standard laboratory conditions. Hence, it one major challenges in microbiology uncover mechanisms required pathway activation. Recently, we discovered intimate physical interaction important model fungus Aspergillus nidulans with soil-dwelling bacterium...
Abstract We report here a recombinant expression system that allows production of large quantities Alzheimer's Aβ(1–40) peptide. The material is competent to dissolve in water solutions with “random‐coil properties,” although its conformation and factual oligomerization state are determined by the physico‐chemical solution conditions. When dissolved 50 mM sodium phosphate buffer (pH 7.4) at 37°C, peptide able undergo nucleated polymerization reaction. aggregation profile characteristically...
Amyloid fibrils are fibrillar polypeptide aggregates from several degenerative human conditions, including Alzheimer's and Creutzfeldt-Jakob diseases. Analysis of amyloid derived various diseases (AA, ATTR, Aβ 2 M, ALλ, ALκ amyloidosis) shows that these associated with a common lipid component has conserved chemical composition is specifically rich in cholesterol sphingolipids, the major components cellular rafts. This pattern not notably affected by purification procedure, no tight...
Abstract Background Millions of humans and animals suffer from superficial infections caused by a group highly specialized filamentous fungi, the dermatophytes, which exclusively infect keratinized host structures. To provide broad insights into molecular basis pathogenicity-associated traits, we report first genome sequences two closely phylogenetically related Arthroderma benhamiae Trichophyton verrucosum , both induce inflammatory in humans. Results 97% 22.5 megabase A. T. are...
Filamentous fungi produce numerous natural products that constitute a consistent source of potential drug leads, yet it seems the majority are overlooked since most biosynthesis gene clusters silent under standard cultivation conditions. Screening secondary metabolite genes model fungus Aspergillus nidulans, we noted cluster on chromosome II comprising two nonribosomal peptide synthetase (NRPS) genes, inpA and inpB, flanked by regulatory named scpR for metabolism cross-pathway regulator. The...
Stimulating encounter: The intimate, physical interaction between the soil-derived bacterium Streptomyces rapamycinicus and human pathogenic fungus Aspergillus fumigatus led to activation of an otherwise silent polyketide synthase (PKS) gene cluster coding for unusual prenylated polyphenol (fumicycline A). meroterpenoid pathway is regulated by a pathway-specific activator as well epigenetic factors.
Although the interaction between prokaryotic and eukaryotic microorganisms is crucial for functioning of ecosystems, information about processes driving microbial interactions within communities remains scarce. Here we show that arginine-derived polyketides (arginoketides) produced by Streptomyces species mediate cross-kingdom with fungi genera Aspergillus Penicillium, trigger production natural products. Arginoketides can be cyclic or linear, a prominent example azalomycin F iranensis,...
The eukaryotic epigenetic machinery can be modified by bacteria to reprogram the response of eukaryotes during their interaction with microorganisms. We discovered that bacterium Streptomyces rapamycinicus triggered increased chromatin acetylation and thus activation silent secondary metabolism ors gene cluster in fungus Aspergillus nidulans. Using this model, we aim understanding mechanisms microbial communication based on bacteria-triggered modification. genome-wide ChIP-seq analysis...
Abstract The formation of polypeptide aggregates represents a nucleated polymerization reaction in which an initial nucleation event (lag phase) is followed by the extension newly formed nuclei into larger aggregates, including fibrils (growth phase). efficiencies these reactions relate to lag time and rate aggregation phase), can be determined from experimental curves. Here we present mutagenic analysis replace valine 18 Alzheimer's Aβ (1–40) peptide with 17 different amino acids determine...
Abstract Organismal interactions within microbial consortia and their responses to harmful intruders remain largely understudied. An important step toward the goal of understanding functional ecological evolutionary selection is study increasingly complex interaction systems. Here, we discovered a tripartite biosystem consisting fungus Aspergillus nidulans, unicellular green alga Chlamydomonas reinhardtii, algicidal bacterium Streptomyces iranensis. Genetic analyses MALDI-IMS demonstrate...
Increasing evidence indicates that polypeptide aggregation often involves a nucleation and growth phase, although the relationship between factors determine these two phases has not yet been fully clarified. We present here an analysis of several mutations at different sites Abeta(1-40) peptide, including those associated with early onset forms Alzheimer's disease, which reveals effects specific amino acid substitutions in sequence this peptide are strongly modulated by their structural...
What makes a fungus blush? The deletion of gene that is required for global protein N-acetylation triggers the production unprecedented metabolites in Aspergillus nidulans. pronounced red pigmentation engineered mutant caused by pheofungins (benzothiazinone chromophores, see scheme), biogenesis which strikingly similar to those pheomelanins found bird feathers and hair Celtic origin.
Basidiomycete fungi are characterized ecologically for their vital functional role in ecosystem carbon recycling and chemically capacity to produce a diverse array of small molecules. Chromophoric natural products derived from the quinone precursor atromentin, such as variegatic acid involutin, have been shown function redox cycling. Yet, context an inter-kingdom system these pigments still elusive. Here, we co-cultured model saprotrophic basidiomycete Serpula lacrymans with ubiquitous...
Hereditary amyloidoses are caused by germline mutations, which increase the propensity of a protein to form cross-β aggregates and deposit as amyloid. particularly interesting they help understand how changes in primary structure an otherwise non-amyloidogenic contribute amyloidogenesis. Here we report on novel systemic ALys amyloidosis, compound heterozygosity exon 2 (p.T70N) 4 (p.W112R) lysozyme gene (LYZ), with both mutations being present same allele. This type hereditary amyloidosis is...
Unite in times of deprivation: We report on the use a chemostat to elicit cryptic biosynthetic pathways fungus. Cutlivation Aspergillus nidulans under N-limiting conditions led specific induction polyketide biosynthesis genes that were otherwise silent. The merger an anthraquinone with orsellinic acid-derived oxanthrene yielded two spiroanthrones, sanghaspirodins A and B (see figure).