Fungi produce numerous low molecular weight molecules endowed with a multitude of biological activities. However, mining the full-genome sequences fungi indicates that their potential to secondary metabolites is greatly underestimated. Because most biosynthesis gene clusters are silent under laboratory conditions, one major challenges understand physiological conditions which these genes activated. Thus, we cocultivated important model fungus Aspergillus nidulans collection 58 soil-dwelling...

Two strains of a new gram-positive coryneform bacterium isolated from soil and sandstone surface are described. Strain 2002-39/1T (T = type strain) is coccoid, nonmotile, non-acid-fast, microaerophilic organism. The menaquinones this strain MK-12 MK-11, the main components whole-cell sugars glucose rhamnose. No mycolic acids present. G+C content DNA 74 mol%. Comparative 16S ribosomal studies cell wall analysis revealed that represents genus belonging to group actinomycetes have...

A novel actinomycete was isolated from compost soil and studied taxonomically phylogenetically. Cells of this organism were gram positive, not acid fast, nonmotile, nonsporulating, irregular coccoid to short rod shaped, microaerophilic. The cell wall peptidoglycan contained lysine cross-linked via an L-Lys<--L-Ser<--D-Asp interpeptide bridge. major menaquinone MK-8(H4). polar lipids phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unknown...

The taxonomic position, growth characteristics and antibiotic resistance properties of a slightly yellow-pigmented bacterial strain, designated R26(T), isolated from the midgut mosquito Anopheles gambiae, were studied. isolate produced rod-shaped cells, which stained Gram-negative. bacterium had two optima at 30-31 °C 37 °C. Strain R26(T) demonstrated natural to ampicillin, chloramphenicol, kanamycin, streptomycin tetracycline. 16S rRNA gene sequence analysis revealed that showed 98.6 %...

Antibiotics from few culturable microorganisms have saved millions of lives since the 20th century. But with resistance formation, these compounds become increasingly ineffective, while majority microbial and that chemical compound diversity remains inaccessible for cultivation exploration. Culturing recalcitrant bacteria is a stochastic process. conventional methods are limited to low throughput. By increasing (i) throughput (ii) sensitivity by miniaturization, we innovate microbiological...

The majority of today's antimicrobial therapeutics is derived from secondary metabolites produced by Actinobacteria. While it generally assumed that less than 1% Actinobacteria species soil habitats have been cultivated so far, classic screening approaches fail to supply new substances, often due limited throughput and frequent rediscovery already known strains. To overcome these restrictions, we implement high-throughput cultivation soil-derived in microfluidic pL-droplets generating more...

A Gram-stain-negative, rod-shaped, non-spore-forming bacterium originating from a human clinical specimen was studied for its taxonomic position. 16S rRNA gene sequence similarity studies clearly allocated this strain (CCUG 58010 T ) to the class Betaproteobacteria , closely related members of genera Massilia and Naxibacter . varians shown be most species on basis (97.5 %), followed by niastensis (96.8 %) aerilata (96.4 %). Similarities all other were in range 93.9–96.2 %. Chemotaxonomic...

Three Gram-staining-negative non-endospore-forming strains were isolated from farmed fish in Chile: one (LM-09-Fp(T)) a rainbow trout (Oncorhynchus mykiss) and the others (LM-19-Fp(T) LM-20-Fp) two Atlantic salmon (Salmo salar). Phylogenetic analyses based on 16S rRNA gene sequences indicated that all three isolates belonged to genus Flavobacterium. In these analyses, strain LM-09-Fp(T) appeared most closely related type of Flavobacterium chungangense (98.5 % sequence similarity), glaciei...

The natural bacterial diversity is regarded as a treasure trove for products. However, accessing complex cell mixtures derived from environmental samples in standardized high-throughput screenings challenging. Here, we present droplet-based microfluidic platform ultrahigh-throughput able to directly harness the of entire microbial communities. This combines extensive cultivation protocols aqueous droplets starting single cells or spores with modular detection methods produced antimicrobial...

The chemical analysis of insect-associated Actinobacteria has attracted the interest natural product chemists in past years as bacterial-produced metabolites are sought to be crucial for sustaining and protecting insect host. objective our study was evaluate phylogeny bioprospecting associated with fungus-growing termites. We characterized 97 from gut, exoskeleton, fungus garden (comb) termite Macrotermes natalensis used two different bioassays assess their general antimicrobial activity....

New gram-positive bacteria were isolated from 1-year-old sludge a wastewater treatment plant. The isolates are coccoid to rod-shaped, nonmotile aerobes that form neither spores nor mycelia. They characterized by peptidoglycan with directly cross-linked meso-diaminopimelic acid (type A1-γ), the presence of menaquinone MK-8(H4). and lack mycolic acids. strains have complex fatty patterns i-C16:0 straight-chain saturated unsaturated acids as major components. G+C content DNA is 70 mol%. results...

A new gram-positive, alkaliphilic, nonsporulating, rod-shaped bacterium is described. The organism was isolated from soda soil (Lake Bogoria, Kenya) and has the following characteristics. It nonmotile, not acid fast, halotolerant, microaerophilic, optimal growth occurs at pH values between 9 10. peptidoglycan type of A4α, with lysine as characteristic diamino glutamic a component interpeptide bridge. major menaquinone MK-8(H4). polar lipids are phosphatidylglycerol, diphosphatidylglycerol,...

An increase in the number of blood γδ T cells follows cytomegalovirus (CMV) infection kidney transplant recipients. These react against CMV-infected and tumor epithelial vitro. We hypothesized that these CMV-induced play a protective role cancer performed longitudinal case-control study involving 18 recipients who developed between 2 6 yr after transplantation 45 did not. The median percentage among total lymphocytes patients with malignancies was significantly lower compared control at 6,...

A Gram-stain-positive, non-endospore-forming rod-shaped bacterium, strain Ca11(T), was isolated from a bioreactor with extensive phosphorus removal and studied in detail for its taxonomic allocation. 16S rRNA gene sequence analysis revealed closest similarity of the to type strains [Corynebacterium hoagii] Rhodococcus equi (98.9%), koreensis wratislaviensis (both 98.4%), opacus canchipurensis 98.0%) followed by kunmingensis imtechensis (97.7%). Phylogenetic trees showed distinct clustering...

Two Gram-negative, rod-shaped bacteria, strains E96(T) and E90(T), were isolated from medicinal leeches (Hirudo verbana) characterized by a polyphasic taxonomic approach. Phylogenetic analysis based on the nearly full-length 16S rRNA gene sequence showed that two shared 98.1% similarity affiliated with genus Niabella within phylum Bacteroidetes, 94.4-97.6% to type of species highest strain aurantiaca (97.3 97.6%, respectively). Niabella-related sequences recently detected in bladders Hirudo...

Seventy-three strains of Sorangium have been isolated from soil samples collected all over the world. The were characterized using a polyphasic approach and phenotypic, genotypic chemotype analyses clarified their taxonomic relationships. 16S rRNA, xynB1, groEL1, matrix-assisted laser desorption/ioniziation time-of-flight mass spectrometry API-ZYM conducted. In addition, selected representative strains, fatty acids, quinones phospholipids analysed. silico DNA-DNA hybridization against...

A Gram-stain-positive, aerobic organism, isolated from a blood sample 51-year-old woman, was studied for its taxonomic position. Based on 16S rRNA gene sequence similarity comparisons, strain CCUG 53762(T) grouped into the genus Alicyclobacillus, most closely related to type of Alicyclobacillus pohliae (94.7 %). The other species ≤91 % and Tumebacillus 91.3-93 %. occurrence menaquinone MK-7 as major respiratory quinone, meso-diaminopimelic acid diagnostic diamino cell wall fatty profile...

Two Gram-positive coccoid, non-motile bacteria with L-ornithine as diagnostic diamino acid of the peptidoglycan and an interpeptide bridge L-Orn<--Gly(1,2)<--D-Glu were isolated from a sample garden soil. The major menaquinone is MK-8(H4). 13-methyl 12-methyl tetradecanoic acids are predominant fatty acids. polar lipids phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylserine two unknown phospholipids. Mycolic absent. DNA base composition 72 mol% G + C. Recent...

A gram-negative, rod-shaped, non-spore-forming bacterium (strain CCUG 43427A(T)) was isolated from a patient suffering endophthalmitis and its taxonomic position studied. 16S rRNA gene sequence analysis indicated that this strain member of the genus Massilia. Strain 43427A(T) most closely related to type strains Massilia timonae (97.4 % similarity) aurea (97.2 %); levels similarity all other recognized species were below 97.0 %. Chemotaxonomic data [Q-8 as major ubiquinone;...

Amphiphilic star-shaped poly(ε-caprolactone)-block-poly(oligo(ethylene glycol)methacrylate) [PCLa-b-POEGMAb]4 block copolymers with four arms and varying degrees of polymerization for the core (PCL) shell (POEGMA) were used to investigate solution behavior in dilute aqueous using a variety techniques, including fluorescence UV/Vis spectroscopy, dynamic light scattering, analytical ultracentrifugation, isothermal titration calorimetry. Particular emphasis has been applied prove that systems...

A Gram-positive-staining, aerobic, endospore-forming bacterium, isolated as a contamination from an enrichment of enteric bacteria surface water, was studied using polyphasic taxonomic approach. 16S rRNA gene sequence similarity comparisons revealed that strain FSt3A(T) grouped in the genus Lysinibacillus, most closely related to Lysinibacillus xylanilyticus XDB9(T) (98.1%), parviboronicapiens BAM-582(T) and sphaericus DSM 28(T) (both 98.0%). The other species <97.5%. allocation supported by...