A5010592951- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Chemical synthesis and alkaloids
- Psychedelics and Drug Studies
- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogens and Fungal Diseases
- Plant biochemistry and biosynthesis
- Plant-Microbe Interactions and Immunity
- Carbohydrate Chemistry and Synthesis
- Enzyme-mediated dye degradation
- Microbial Metabolism and Applications
- Alkaloids: synthesis and pharmacology
- Plant Pathogenic Bacteria Studies
- Chemical Synthesis and Analysis
- Polyamine Metabolism and Applications
- Genomics and Phylogenetic Studies
- Mycotoxins in Agriculture and Food
- Glycosylation and Glycoproteins Research
- Fungal and yeast genetics research
- Fermentation and Sensory Analysis
- Synthetic Organic Chemistry Methods
- Forest Ecology and Biodiversity Studies
- Phytochemistry and Bioactivity Studies
- Cholinesterase and Neurodegenerative Diseases
- Legume Nitrogen Fixing Symbiosis
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)
2016-2025
Friedrich Schiller University Jena
2015-2024
Schiller International University
2011-2019
University of Minnesota
2008-2009
University of Freiburg
2001-2008
University of Cologne
2007
Institute for Biomedical and Pharmaceutical Research
2007
University of Kentucky
2003-2005
University of Wisconsin–Madison
2003-2004
Highland Community College - Illinois
2004
Comparative genomic analysis of “dry rot” fungus shows both convergent evolution and divergence among fungal decomposers.
Summary Ectomycorrhizal fungi are thought to have a key role in mobilizing organic nitrogen that is trapped soil matter ( SOM ). However, the extent which ectomycorrhizal decompose and mechanism by they do so remain unclear, considering lost many genes encoding lignocellulose‐degrading enzymes present their saprotrophic ancestors. Spectroscopic analyses transcriptome profiling were used examine mechanisms five species of fungi, representing at least four origins symbiosis, extracted from...
Efficient lignin depolymerization is unique to the wood decay basidiomycetes, collectively referred as white rot fungi. Phanerochaete chrysosporium simultaneously degrades and cellulose, whereas closely related species, Ceriporiopsis subvermispora, also depolymerizes but may do so with relatively little cellulose degradation. To investigate basis for selective ligninolysis, we conducted comparative genome analysis of C. subvermispora P. . Genes encoding manganese peroxidase numbered 13 five...
• Parasitism and saprotrophic wood decay are two fungal strategies fundamental for succession nutrient cycling in forest ecosystems. An opportunity to assess the trade-off between these is provided by pathogen decayer Heterobasidion annosum sensu lato. We report annotated genome sequence transcript profiling, as well quantitative trait loci mapping, of one member species complex: H. irregulare. Quantitative critical pathogenicity, rich transposable elements, orphan secreted genes, were...
Abstract Psilocybin is the psychotropic tryptamine‐derived natural product of Psilocybe carpophores, so‐called “magic mushrooms”. Although its structure has been known for 60 years, enzymatic basis biosynthesis remained obscure. We characterized four psilocybin enzymes, namely i) PsiD, which represents a new class fungal l ‐tryptophan decarboxylases, ii) PsiK, catalyzes phosphotransfer step, iii) methyltransferase PsiM, catalyzing iterative N‐methyl transfer as terminal biosynthetic and iv)...
Clonostachys rosea is a mycoparasitic fungus that can control several important plant diseases. Here, we report on the genome sequencing of C. and comparative analysis, in order to resolve phylogenetic placement study evolution mycoparasitism as fungal lifestyle. The estimated 58.3 Mb, contains 14,268 predicted genes. A phylogenomic analysis shows clusters sister taxon pathogenic Fusarium species, with mycoparasitic/saprotrophic Trichoderma species an ancestral position. gene family reveals...
Biosynthetic crosstalk: Most gene clusters in fungi are orphans with no known associated metabolites. NMR-based comparative metabolomics was used to identify the products of two highly homologous orphan Aspergillus flavus. The encode partially redundant biosynthetic pathways that produce overlapping sets novel fungal alkaloids, feature NRPS-like genes unusual functions, and regulate development. As a service our authors readers, this journal provides supporting information supplied by...
Abstract Aspirochlorine ( 1 ) is an epidithiodiketopiperazine (ETP) toxin produced from koji mold (Aspergillus oryzae), which has been used in the oriental cuisine for over two millennia. Considering its potential risk food safety, we have elucidated molecular basis of aspirochlorine biosynthesis. By a combination genetic and chemical analyses found acl gene locus identified key role AclH as chlorinase. Stable isotope labeling, biotransformation, mutational experiments, analysis...
Collectively classified as white-rot fungi, certain basidiomycetes efficiently degrade the major structural polymers of wood cell walls. A small subset these Agaricomycetes, exemplified by Phlebiopsis gigantea, is capable colonizing freshly exposed conifer sapwood despite its high content extractives, which retards establishment other fungal species. The mechanism(s) P. gigantea tolerates and metabolizes resinous compounds have not been explored. Here, we report annotated genome compare...
Abstract Psilocybin, the natural hallucinogen produced by Psilocybe (“magic”) mushrooms, holds great promise for treatment of depression and several other mental health conditions. The final step in psilocybin biosynthetic pathway, dimethylation tryptophan-derived intermediate norbaeocystin, is catalysed PsiM. Here we present atomic resolution (0.9 Å) crystal structures PsiM trapped at various stages its reaction cycle, providing detailed insight into SAM-dependent methylation mechanism....
Heterologous expression of multi-gene biosynthetic pathways in eukaryotic hosts is limited by highly regulated individual monocistrons. Dissimilar to prokaryotes, each gene strictly controlled its own regulatory elements, such as promoter and terminator. Consequently, parallel transcription can occur only when a group genes synchronously activated. A strategy circumvent this limitation the concerted multiple polycistron. By exploiting "stop-carry on" mechanism picornaviruses, we have...
Prenyltransferases (PTs) are involved in the biosynthesis of a multitude pharmaceutically and agriculturally important plant, bacterial, fungal compounds. Although numerous prenylated compounds have been isolated from Basidiomycota (mushroom-forming fungi), knowledge PTs catalyzing transfer reactions this group fungi is scarce. Here, we report biochemical characterization an O- C-prenylating dimethylallyltryptophan synthase (DMATS)-like enzyme LpTyrPT scurfy deceiver Laccaria proxima. This...
Sweet success: A natural product glycosyltransferase, UrdGT2 from Streptomyces fradiae Tü2717, is the first glycosyltransferase able to catalyze both C-glycosidic and O-glycosidic sugar transfers. These results shed new light on mechanisms of C-glycosylation aromatic compounds, which significant for chemoenzymatic drug lead derivatization as well enzymology.
Ectomycorrhizal fungi play a key role in mobilizing nutrients embedded recalcitrant organic matter complexes, thereby increasing nutrient accessibility to the host plant. Recent studies have shown that during assimilation of nutrients, ectomycorrhizal fungus Paxillus involutus decomposes using an oxidative mechanism involving Fenton chemistry (Fe(2+) + H2O2 H(+) → Fe(3+) ˙OH H2O), similar brown rot wood-decaying fungi. In such fungi, secreted metabolites are one components drive one-electron...