A5041084992- Chemical Synthesis and Reactions
- Synthetic Organic Chemistry Methods
- Chemical Synthesis and Analysis
- Enzyme-mediated dye degradation
- Mycobacterium research and diagnosis
- Asymmetric Hydrogenation and Catalysis
- Multicomponent Synthesis of Heterocycles
- Oxidative Organic Chemistry Reactions
- Asymmetric Synthesis and Catalysis
- Catalytic Cross-Coupling Reactions
- Sulfur-Based Synthesis Techniques
- Microbial Natural Products and Biosynthesis
- Catalytic C–H Functionalization Methods
- Synthesis and Catalytic Reactions
- Cyclopropane Reaction Mechanisms
- Fungal Biology and Applications
- Microwave-Assisted Synthesis and Applications
- Nanomaterials for catalytic reactions
- Catalytic Alkyne Reactions
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Click Chemistry and Applications
- Synthesis of Indole Derivatives
- Carbohydrate Chemistry and Synthesis
- Microbial Metabolism and Applications
University of Cincinnati Medical Center
2014-2025
University of Cincinnati
2013-2024
Indian Institute of Chemical Technology
2007-2016
University of Hyderabad
2015-2016
Institut Català d'Investigació Química
2013-2016
Philadelphia University
2014
Alabama State University
2014
Academy of Scientific and Innovative Research
2014
National Center for Toxicological Research
2013
Universitat de Barcelona
2012
Wood is a major pool of organic carbon that highly resistant to decay, owing largely the presence lignin. The only organisms capable substantial lignin decay are white rot fungi in Agaricomycetes, which also contains non-lignin-degrading brown and ectomycorrhizal species. Comparative analyses 31 fungal genomes (12 generated for this study) suggest lignin-degrading peroxidases expanded lineage leading ancestor reconstructed as species, then contracted parallel lineages mycorrhizal Molecular...
Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and also largely responsible for the destructive decay wooden structures. Rapid depolymerization cellulose is a distinguishing feature brown-rot, but biochemical mechanisms underlying genetics poorly understood. Systematic examination P. genome, transcriptome, secretome revealed unique extracellular enzyme systems, including an unusual repertoire glycoside hydrolases. Genes encoding exocellobiohydrolases...
Efficient lignin depolymerization is unique to the wood decay basidiomycetes, collectively referred as white rot fungi. Phanerochaete chrysosporium simultaneously degrades and cellulose, whereas closely related species, Ceriporiopsis subvermispora, also depolymerizes but may do so with relatively little cellulose degradation. To investigate basis for selective ligninolysis, we conducted comparative genome analysis of C. subvermispora P. . Genes encoding manganese peroxidase numbered 13 five...
Evolution of lignocellulose decomposition was one the most ecologically important innovations in fungi. White-rot fungi Agaricomycetes (mushrooms and relatives) are effective microorganisms degrading both cellulose lignin components woody plant cell walls (PCW). However, precise evolutionary origins poorly understood, largely because certain early-diverging clades its sister group, Dacrymycetes, have yet to be sampled, or been undersampled, comparative genomic studies. Here, we present new...
We report the development and initial validation of a paper-based nucleic acid testing platform that integrates Loop-mediated isothermal amplification (LAMP) with clustered regularly interspaced short palindromic repeats (CRISPR) technology, referred to as PLACID (Paper-based LAMP-CRISPR Integrated Diagnostics). LAMP eliminates need for thermal cycling, resulting in simplified instrumentation, CRISPR-associated protein (Cas 12a) system false positive signals from products, highly selective...
Degradation of the BTEX (benzene, toluene, ethylbenzene, and o-, m-, p-xylenes) group organopollutants by white-rot fungus Phanerochaete chrysosporium was studied. Our results show that organism efficiently degrades all components when these compounds are added either individually or as a composite mixture. favored under nonligninolytic culture conditions in malt extract medium, which extracellular lignin peroxidases (LIPs) manganese-dependent (MNPs) not produced. The noninvolvement LIPs...
Abstract Background Softwood is the predominant form of land plant biomass in Northern hemisphere, and among most recalcitrant resources to bioprocess technologies. The white rot fungus, Phanerochaete carnosa , has been isolated almost exclusively from softwoods, while other known white-rot species, including chrysosporium were mainly hardwoods. Accordingly, it anticipated that P. encodes a distinct set enzymes proteins promote softwood decomposition. To elucidate genetic basis bioconversion...
A chemically modified, first generation MacMillan imidazolidin-4-one has been anchored onto 1% DVB Merrifield resin and Fe3O4 (5.3 ± 1.4 nm) magnetic nanoparticles through copper-catalyzed alkyne azide cycloaddition (CuAAC) reactions. The resulting immobilized catalysts have successfully used in the asymmetric Friedel-Crafts alkylation of N-substituted pyrroles with α,β-unsaturated aldehydes. PS-supported catalyst (B) showed higher catalytic activity enantioselectivity, while MNP-supported...
Collectively classified as white-rot fungi, certain basidiomycetes efficiently degrade the major structural polymers of wood cell walls. A small subset these Agaricomycetes, exemplified by Phlebiopsis gigantea, is capable colonizing freshly exposed conifer sapwood despite its high content extractives, which retards establishment other fungal species. The mechanism(s) P. gigantea tolerates and metabolizes resinous compounds have not been explored. Here, we report annotated genome compare...
A novel and efficient reduction of various prochiral ketones such as acetopehones, α-azido aryl ketones, β-ketoesters, aliphatic acyclic cyclic to the corresponding optically acive secondary alcohols with moderate excellent chemical yield was achieved by using Daucus carota, root plant cells under extremely mild environmentally benign conditions in aqueous medium, has been described. Many these active are potential chiral building blocks for synthesis pharmaceutically important molecules...
A convenient and efficient synthesis of 1,2-azidoalcohols 1,2-azidoamines has been achieved by ring opening epoxides aziridines using cerium(III) chloride sodium azide in acetonitrile. The reaction is highly regioselective afforded the corresponding products good to excellent yields under mild neutral conditions. method very rapid equally compatible for both aziridines.
Evidence for substantial degradation of polychlorinated biphenyl mixtures Aroclor 1242, 1254, and 1260 by the white rot fungus Phanerochaete chrysosporium, based on congener-specific gas chromatographic analysis, is presented. Maximal (percent weight) Aroclors was 60.9, 30.5, 17.6%, respectively. Most congeners in 1242 1254 were degraded extensively both low-N (ligninolytic) as well high-N (nonligninolytic) defined media. Even more extensive observed malt extract medium. Congeners with...
Cytochrome P450 monooxygenases (P450s) are known to oxidize hydrocarbons, albeit with limited substrate specificity across classes of these compounds. Here we report a monooxygenase (CYP63A2) from the model ligninolytic white rot fungus Phanerochaete chrysosporium that was found possess broad oxidizing capability toward structurally diverse hydrocarbons belonging mutagenic/carcinogenic fused-ring higher-molecular-weight polycyclic aromatic (HMW-PAHs), endocrine-disrupting long-chain...
Herein we present magnetically recoverable and reusable copper ferrite nanoparticles for asymmetric hydrosilylation of several ketones. Up to 99% enantiometric excess was obtained at room temperature using polymethylhydrosiloxane as the stoichiometric reducing agent. The were separated, efficiency catalyst remains almost unaltered up three cycles.
Trifunctional N,N,O-terdentate amido/pyridyl carboxylate Pd(II) complexes were highly active and stable phosphine-free catalysts for Heck room-temperature Suzuki reactions with high turnover numbers up to ca. 104.
Abstract Background Phanerochaete chrysosporium , the model white rot basidiomycetous fungus, has extraordinary ability to mineralize (to CO 2 ) lignin and detoxify a variety of chemical pollutants. Its cytochrome P450 monooxygenases have recently been implied in several these biotransformations. Our initial cloning efforts P. its subsequent whole genome sequencing revealed an repertoire ("P450ome") containing at least 150 genes with yet unknown function. In order understand functional...