A5101915948- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Axon Guidance and Neuronal Signaling
- DNA and Nucleic Acid Chemistry
- Neurogenesis and neuroplasticity mechanisms
- Electrochemical sensors and biosensors
- Transgenic Plants and Applications
- Plant Virus Research Studies
- Gold and Silver Nanoparticles Synthesis and Applications
- SARS-CoV-2 detection and testing
- Molecular Sensors and Ion Detection
- Analytical Chemistry and Sensors
- CRISPR and Genetic Engineering
- Developmental Biology and Gene Regulation
- Genetics and Neurodevelopmental Disorders
- Protein Kinase Regulation and GTPase Signaling
- Molecular Junctions and Nanostructures
- Adipose Tissue and Metabolism
- Advanced Biosensing Techniques and Applications
- Metabolism, Diabetes, and Cancer
Institute of Environmental Science and Research
2025
Victoria University of Wellington
2022-2023
MacDiarmid Institute for Advanced Materials and Nanotechnology
2022-2023
Delhi Technological University
2016
Zen Bio (United States)
2002
Duke Medical Center
2001-2002
Duke University Hospital
2001-2002
Triangle
2002
Howard Hughes Medical Institute
2001-2002
We report the development and initial validation of a paper-based nucleic acid testing platform that integrates Loop-mediated isothermal amplification (LAMP) with clustered regularly interspaced short palindromic repeats (CRISPR) technology, referred to as PLACID (Paper-based LAMP-CRISPR Integrated Diagnostics). LAMP eliminates need for thermal cycling, resulting in simplified instrumentation, CRISPR-associated protein (Cas 12a) system false positive signals from products, highly selective...
Colorimetric gold-nanoparticle-based biosensors are an attractive platform for the detection of small-molecule analytes. Taking advantage adsorption DNA aptamer probes on AuNPs, these sensors can be simple, rapid, sensitive, selective, and cost-effective. These properties important rapid drugs like methamphetamine in biological matrices. Saliva is a highly desirable matrix development diagnostic tests because saliva sampling minimally invasive drug levels relate to recent use rather than...
Thiolated DNA biopolymer probes are widely used for their spontaneous interactions with gold electrodes to achieve self-assembled monolayers (SAMs) of DNA. This offers an attractive class bio-interfaces developing point-of-care (POC) diagnostics. However, SAMs prone structural instability and can be challenging reproducibly fabricate different sizes shapes. Among methods studying SAMs, electrochemical impedance spectroscopy (EIS) has attracted a lot attention its extremely high sensitivity...
Doublecortin is a cytoplasmic protein mutated in the neuronal migration disorder X-linked lissencephaly. This study describes novel activity of doublecortin recognition FIGQY-phosphotyrosine motif present domain L1 cell adhesion molecule neurofascin. Phospho-FIGQY-neurofascin (186 kDa) coimmunoprecipitated with from detergent extracts embryonic brain membranes, and this doublecortin-phospho-FIGQY neurofascin complex was disassociated by synthetic phospho-FIGQY peptide but not dephospho-FIGQY...
Insulin-stimulated glucose transport is impaired in the early phases of type 2 diabetes mellitus. Studies rodent cells suggest that atypical PKC (aPKC) isoforms (ζ, λ, and ι) PKB, their upstream activators, PI3K 3-phosphoinositide-dependent protein kinase-1 (PDK-1), play important roles insulin-stimulated transport. However, there no information on requirements for aPKCs, or PDK-1 during insulin action human cell types. Presently, by using preadipocyte-derived adipocytes, we were able to...
Phosphorylation of neurofascin, a member the L1 family cell adhesion molecules (L1 CAMs), at conserved FIGQY-tyrosine abolishes ankyrin-neurofascin interaction. This study provides first evidence, in Drosophila melanogaster and vertebrates, for physiological occurrence FIGQY phosphorylation members. tyrosine is localized specialized junctions, including paranodes sciatic nerve, neuromuscular junctions adult rats embryos, epidermal muscle attachment sites Drosophila, adherens developing...
We report results of studies relating to the fabrication a flexible, disposable, and label free biosensing platform for detection cancer biomarker (carcinoembryonic antigen, CEA). Polyaniline (PANI) has been electrochemically deposited over gold sputtered paper (Au@paper) covalent immobilization monoclonal carcinoembryonic antibodies (anti-CEA). The bovine serum albumin (BSA) used blocking nonspecific binding sites at anti-CEA conjugated PANI/Au@Paper. PANI/Au@Paper, anti-CEA/PANI/Au@Paper,...
DNA aptamers have emerged as promising probes for challenging analytes that cannot be easily detected by conventional probes, including small-molecule targets. Among the different signal transduction approaches, gold nanoparticle (AuNP) aggregation assays been widely used to generate a colorimetric response from aptamer–target interactions. This sensor design relies on competition between aptamer adsorbing AuNP surface versus interacting with target, whereby target binding reduces number of...
Abstract Nucleic acid isothermal amplification methods are advantageous for point-of-care (POC) diagnostics because of their precision, sensitivity, and low power requirements. Loop-mediated (LAMP) is an established method, renowned its nucleic efficiency robust semi purified target acids. However, LAMP may be prone to non-specific causing false positives therefore fails replace PCR as the gold standard method in clinical testing. We show that combined with clustered regularly interspaced...