A5052946397- Parathyroid Disorders and Treatments
- Genetic Syndromes and Imprinting
- Chronic Kidney Disease and Diabetes
- Cellular transport and secretion
- Kruppel-like factors research
- Fungal Biology and Applications
- Fungal and yeast genetics research
- Bacterial biofilms and quorum sensing
- Diabetes Treatment and Management
- Renal Diseases and Glomerulopathies
- Aortic Thrombus and Embolism
- Microbial Natural Products and Biosynthesis
- Algal biology and biofuel production
- Bacterial Genetics and Biotechnology
- Endoplasmic Reticulum Stress and Disease
- Fibroblast Growth Factor Research
- Connexins and lens biology
- Body Contouring and Surgery
- Renal and related cancers
- Case Reports on Hematomas
- Hypertrophic osteoarthropathy and related conditions
- Lichen and fungal ecology
- Cardiovascular Health and Disease Prevention
- Plant-Microbe Interactions and Immunity
- Circadian rhythm and melatonin
Hospital Universitario Nuestra Señora de Candelaria
2019-2023
Universidad de La Laguna
2021-2023
Estación Experimental del Zaidín
2016-2022
Universidad de Sevilla
2012-2019
Consejo Superior de Investigaciones Científicas
2016
Pacific Northwest National Laboratory
2012
University of Groningen
2012
Karlsruhe Institute of Technology
2012
Significance Photolyases repair UV-B–induced DNA lesions. They form a large protein family together with cryptochrome photoreceptors (cryptochrome/photolyase family, CPF). A more recently discovered CPF subclade consists of DASH ( Drosophila , Arabidopsis Synechocystis Human)-type cryptochromes (cry-DASH), present in bacteria, plants, animals, and fungi. Cry-DASH are considered as residual activity for lesions single-stranded DNA. Canonical photolyases such double-stranded Here, we show that...
Decrease in soluble anti-aging Klotho protein levels is associated to cardiovascular disease (CVD).Diverse studies have shown a bidirectional relationship between and inflammation, risk factor for the development of CVD.In this work we aimed evaluate association inflammatory cytokines context human CVD.The study included 110 patients with established CVD preserved renal function, control group 22 individuals without previous history events.Serum IL10 were significantly lower...
Abstract Cardiovascular disease is the leading cause of death worldwide. New therapeutic strategies are aimed to modulate athero-inflammatory process that partially orchestrates underlying vascular damage. Peripheral blood circulating cells include different immune with a central role in development atherogenic inflammatory response. The anti-aging protein α-Klotho has been related protective effects against CVD. KL expressed monocytes, macrophages, and lymphocytes where it exerts...
Significance Ypt1 and Ypt31/32 RAB GTPases regulate traffic across the Golgi. Both are activated by TRAPP, an oligomeric GEF. Three TRAPP versions share same core subunits. TRAPPI TRAPPIII activate Ypt1. The third, TRAPPII, composed of plus specific subunits, appears to act specifically on Ypt31, but this role has been disputed. By combining resolving power fungal genetics with biochemical assays, we establish that physiological target TRAPPII is RabE, Aspergillus Ypt31 ortholog. However,...
The ascomycete fungus Neurospora is present in many parts of the world, particular tropical and subtropical areas, where it found growing on recently burned vegetation. We have sampled population across Spain. sampling sites were located region Galicia (northwestern corner Iberian peninsula), province Cáceres, city Seville, two major islands Canary Islands archipelago (Tenerife Gran Canaria, west coast Africa). covered a latitude interval between 27.88° 42.74°. identified wild-type strains...
Most bacteria grow in nature forming multicellular structures named biofilms. The bacterial second messenger cyclic diguanosine monophosphate (c-di-GMP) is a key player the regulation of transition from planktonic to sessile lifestyles and this crucial development In Pseudomonas putida KT2440, Rup4959, multidomain response regulator with diguanylate cyclase activity, when overexpressed causes an increment intracellular levels c-di-GMP that gives rise pleiotropic phenotype consisting...
Asymptomatic hyperuricaemia (AHU) is associated with inflammatory disorders, including cardiovascular disease. Uric acid (UA) lowering therapies may reduce the risk of appearance or progression these comorbidities. In this work, we investigated relationship between serum UA levels and inflammation in subjects AHU.Serum high-sensitivity CRP (hsCRP), TNF-α IL-6, mRNA expression TNFa IL6 peripheral blood mononuclear cells were measured individuals AHU without comorbid conditions a control group...
Vascular calcification is a major risk for cardiovascular disease and implies the transformation of smooth muscle cells to an osteoblastic phenotype as consequence dysregulation calcium phosphate metabolism.Fibroblast growth factor (FGF) 23 most potent regulator.Observational studies suggest that high levels FGF23 are related morbidity mortality.In this work, we determined both intact carboxi-terminal fragments circulating in 133 patients with established disease, expression FGF23, its...
Summary In Pseudomonas putida KT2440, cfcR encodes an orphan multidomain response regulator with diguanylate cyclase activity, which is responsible for the synthesis of c‐di‐GMP, a second messenger key in transition from planktonic to sessile bacterial lifestyles. When overexpressed, enhances biofilm formation and causes other phenotype alterations. The cfcA gene, encoding membrane‐anchored multisensory CHASE3/GAF hybrid histidine kinase (HK), required develop this pleiotropic phenotype....
Klotho protein is predominantly expressed in the kidneys and has also been detected vascular tissue peripheral blood circulating cells to a lesser extent. Carotid artery intima-media thickness (CIMT) burden, marker of subclinical atherosclerosis, associated with reductions levels chronic kidney disease patients, who show reduced this at all stages disease. However, contribution serum its expression carotid wall on CIMT absence impairment not yet evaluated.We conducted single-center study 35...
Diabetic foot syndrome (DFS) is a prevalent complication in the diabetic population and major cause of hospitalizations. Diverse clinical studies have related alterations system formed by fibroblast growth factor (FGF)-23 Klotho (KL) with vascular damage. In this proof-of-concept study, we hypothesize that levels FGF23 are altered DFS patients.Twenty patients limb amputation due to DFS, 37 without 12 non-diabetic cadaveric organ donors were included study. Serum FGF23/Klotho inflammatory...
Uso1/p115 and RAB1 tether ER-derived vesicles to the Golgi. contains a globular-head-domain (GHD), coiled-coil (CC) mediating dimerization/tethering, C-terminal region (CTR) interacting with golgins. is recruited by RAB1. Genetic studies placed Uso1 paradoxically acting upstream of, or in conjunction (Sapperstein et al., 1996). We selected two missense mutations uso1 resulting E6K G540S GHD that rescued lethality of rab1 -deficient Aspergillus nidulans . The are phenotypically additive,...
Hyperphosphatemia is commonly present in end-stage renal disease. Klotho (KL) implicated phosphate homeostasis since it acts as obligate co-receptor for the fibroblast growth factor 23 (FGF23), a major phosphaturic hormone. We hypothesized that genetic variation KL gene might be associated with alterations resulting hyperphosphatemia. performed sequencing determining variants group of resistant hyperphosphatemic dialysis patients. In 67-year-old female, blood DNA revealed heterozygous...
Abstract Background and Aims Klotho (KL) is a transmembrane protein that expressed the highest in tubular cells of kidneys. In addition to membrane KL, secreted form this present blood urine generated by ectodomain shedding KL from cell surface or gene through alternative splicing. Preserved expression has been related physiological protection human Sodium-glucose cotransporter 2 inhibitors (SGLT2i) are new class antidiabetic drugs with important renal benefits. The aim proof-of-concept...
Abstract Cardiovascular disease (CVD) is the leading cause of death worldwide. New therapeutic strategies are aimed to modulate athero-inflammatory process that partially orchestrates underlying vascular damage. Peripheral blood circulating cells (PBCCs) include different immune with a central role in development atherogenic inflammatory response. The anti-aging factor α-Klotho (KL) has been related protective effects against CVD. KL expressed monocytes, macrophages and lymphocytes where it...
Background: Vascular calcification (VC) is a major risk for cardiovascular disease and implies the transformation of smooth muscle cells to an osteoblastic phenotype as consequence dysregulation calcium phosphate metabolism. Fibroblast growth factor (FGF) 23 most potent regulator. Observational studies suggest that high levels FGF23 may exert direct effects on system. Methods: We determined expression FGF23, its receptors 1 3, co-receptor KLOTHO in different vascular territories group...