A5075014072- Fungal and yeast genetics research
- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Bacterial Genetics and Biotechnology
- Protist diversity and phylogeny
- RNA and protein synthesis mechanisms
- Plant-Microbe Interactions and Immunity
- Genomics and Phylogenetic Studies
- Plant Pathogens and Fungal Diseases
- Biofuel production and bioconversion
- Antimicrobial Resistance in Staphylococcus
- Probiotics and Fermented Foods
- Polyamine Metabolism and Applications
- Enzyme Production and Characterization
- Plant tissue culture and regeneration
- Enzyme Catalysis and Immobilization
- Microbial Metabolism and Applications
- Mycotoxins in Agriculture and Food
- Endometriosis Research and Treatment
- Plant Pathogenic Bacteria Studies
- Chromium effects and bioremediation
- Phytase and its Applications
- Algal biology and biofuel production
- Enzyme Structure and Function
- Plant and fungal interactions
Universidad Europea del Atlántico
2020-2024
Fundación Universitaria Iberoamericana
2020-2024
Universidad de Sevilla
2014-2021
Instituto de Biotecnología de León
1995-2014
Hospital Universitario Virgen del Rocío
2014
Universidad de León
1994-2006
Abstract Acetylation of histones is a key regulatory mechanism gene expression in eukaryotes. GcnE an acetyltransferase Aspergillus nidulans involved the acetylation histone H3 at lysine 9 and 14. Previous works have demonstrated that deletion gcnE results defects primary secondary metabolism. Here we unveil role development show ∆gcnE mutant strain has minor growth but impaired normal conidiophore development. No signs conidiation were found after 3 days incubation, immature aberrant...
Summary Nitric oxide ( NO ) is a signalling molecule involved in many biological processes bacteria, plants and mammals. However, little known about the role biosynthesis of fungi. Here we show that production increased at early stages transition from vegetative growth to development A spergillus nidulans . Full requires functional nitrate reductase NR gene nia D upregulated upon induction conidiation, even under N ‐repressing conditions presence ammonium. At this stage, homeostasis achieved...
Aspergillus nidulans kdmA encodes a member of the KDM4 family jumonji histone demethylase proteins, highly similar to metazoan orthologues both within functional domains and in domain architecture. This proteins exhibits activity towards lysines 9 36 H3 plays prominent role gene expression chromosome structure many species. Mass spectrometry mapping A. histones revealed that around 3% bulk carried trimethylated H3K9 (H3K9me3) but more than 90% either H3K36me2 or H3K36me3. KdmA functions as...
A homolog of the Corynebacterium diphtheriae dtxR gene was isolated from Brevibacterium lactofermentum. The product B. lactofermentum immunoreactive with polyclonal anti-DtxR antibodies and functioned as an iron-activated repressor capable regulating expression beta-galactosidase a diphtheria tox promoter/operator transcriptional fusion in recombinant Escherichia coli. extents induction by increasing concentrations chelator 2,2'-dipyridyl were identical cells expressing DtxR either C. or
Methionine stimulated cephalosporin production in cultures of three different strains Acremonium chrysogenum when added either at inoculation time or 72 h to cells grown previously the absence methionine. When methionine was h, stimulation biosynthesis observed only 12 later and required de novo protein synthesis. increased levels enzymes (isopenicillin N synthase deacetylcephalosporin C acetyltransferase) expressed from genes (pcbC cefG, respectively) located two clusters wild-type A....
Fossil fuels are consumed so rapidly that it is expected the planet resources will be soon exhausted. Therefore, imperative to develop alternative and inexpensive new technologies produce sustainable fuels, for example biodiesel. In addition hydrolytic esterification reactions, lipases capable of performing transesterification reactions useful production However selection not easy consequently very few biodiesel producing currently available. this work we first isolated 1,016 lipolytic...
Abstract Background Aspergillus spp. comprises a very diverse group of lower eukaryotes with high relevance for industrial applications and clinical implications. These multinucleate species are often cultured many generations in the laboratory, which can unknowingly propagate hidden genetic mutations. To assess likelihood such events, we studied genome stability aspergilli by using combination mutation accumulation (MA) lines whole sequencing. Results We sequenced genomes 30 asexual 10...
Abstract Filamentous fungi naturally grow on solid surfaces, yet most genetic and biochemical analyses are still performed in liquid cultures. Here, we report a multiplexing platform using high-throughput photometric continuous reading that allows parallel quantification of hyphal growth reporter gene expression directly medium, thereby mimicking natural environmental conditions. Using this system, have quantified fungal secondary metabolite GFP-based genes saprophytic Aspergillus...
Summary: Glucose repressed transcription of the penicillin biosynthesis genes pcbAB, pcbC and penDE when added at inoculation time to cultures Penicillium chrysogenum AS-P-78 but it had little repressive effect 12 h no 24 or 36 h. A slight increase in expression (and a smaller extent pcbAB) was observed glucose-grown pH 6·8, 7·4 8·0 as compared with 6·2, alkaline pHs did not override strong repression exerted by glucose. Transcription actin gene used control significantly affected glucose...
The first two genes pcbAB andpcbC of the penicillin biosynthesis pathway are expressed from a 1.01-kilobase bidirectional promoter region. A series sequential deletions were made in region, and constructions with modified promoters coupled to lacZ reporter gene introduced as single copies at pyrG locus Penicillium chrysogenum npe10. Three regions, boxes A, B, C, produced significant decrease expression when deleted. Protein-DNA complexes observed by using electrophoretic mobility shift assay...
Abstract The analysis of filamentous fungi by flow cytometry has been impossible to date due their nature and size. In this work, we have developed a method that combines single-spore microencapsulation large-particle as powerful alternative for the genetic fungi. Individual spores were embedded in monodisperse alginate microparticles incubated appropriate conditions. Growth could be monitored light or fluorescent microscopy Complex Object Parametric Analyzer Sorter cytometry....
Four rpoD hybridizing signals have been identified in the chromosome of Brevibacterium lactofermentum. Two rpoD-like genes, sigA and sigB, cloned sequenced, they encode principal sigma factors RNA polymerase. The deduced amino acid sequences SigA SigB showed very high similarities to those Mycobacterium smegmatis MysA MysB proteins, respectively, also HrdB proteins from different Streptomyces species. maintain conserved motifs 70-like factors. sigB is closely linked dtxR gene (encoding a...
Summary Nitric oxide (NO) is a signalling molecule in eukaryotic and prokaryotic organisms. NO levels transiently boost upon induction of conidiation Aspergillus nidulans . Only one pathway for synthesis involving nitrate reductase has been reported filamentous fungi so far, but this does not satisfy all the produced fungal cells. Here we provide evidence at least additional biosynthetic A l ‐arginine or an intermediate metabolite as substrate. Under certain growth conditions, addition to...
Abstract Background Distal motor neuropathies with a genetic origin have heterogeneous clinical presentation overlapping features affecting distal nerves and including spinal muscular atrophies amyotrophic lateral sclerosis. This indicates that their background is heterogeneous. Patient methods In this work, we identified characterized the molecular base of patient sensorimotor neuropathy unknown origin. For study, performed whole‐exome sequencing, modelling, cloning expression mutant gene,...
Abstract Background αB‐crystallin is a promiscuous protein involved in numerous cell functions. Mutations CRYAB have been found patients with different pathological phenotypes that are not properly understood. Patients can present diseases like cataracts, muscle weakness, myopathy, cardiomyopathy, respiratory insufficiency or dysphagia, but also variable combination of these pathologies has found. These mutations show either autosomal dominant recessive mode inheritance and penetrance...
Five mutants of Penicillium chrysogenum blocked in penicillin biosynthesis (npe) which are deficient isopenicillin N-acyltransferase were isolated previously. Three these mutants, npe6, npe7, and npe8, have been characterized at the molecular level compared with npe10, a deletion mutant. Transcripts normal size (1.15 kb) penDE genes, encode N-acyltransferase, also pcbAB (11.5 pcbC (1.1 genes observed all except for npe10 Immunoblotting studies using antibodies against showed that (except...
Transcription of the sigA and sigB genes Brevibacterium lactofermentum, encoding principal sigma factors RNA polymerase, has been investigated by Northern blot primer extension analysis. hybridizations revealed that is transcribed as a monocistronic mRNA 1.7 kb gives two transcripts 1.1 1.5 kb. Similar transcription patterns in nutrient-rich medium were observed; both occurred simultaneously throughout exponential growth phase decayed clearly when stationary was reached. Primer analysis B....