A5001627451- Plant Pathogens and Fungal Diseases
- Mycorrhizal Fungi and Plant Interactions
- Plant-Microbe Interactions and Immunity
- Biofuel production and bioconversion
- Marine and coastal plant biology
- Fungal and yeast genetics research
- Marine Biology and Ecology Research
- Cancer, Hypoxia, and Metabolism
- Growth Hormone and Insulin-like Growth Factors
- Yeasts and Rust Fungi Studies
- Fungal Biology and Applications
- Macrophage Migration Inhibitory Factor
- Glycosylation and Glycoproteins Research
- Polysaccharides and Plant Cell Walls
- Coastal wetland ecosystem dynamics
- Race, Genetics, and Society
- Signaling Pathways in Disease
- Genetic diversity and population structure
- Microbial Metabolism and Applications
- Bone and Dental Protein Studies
- Food Industry and Aquatic Biology
- Alkaline Phosphatase Research Studies
- Barrier Structure and Function Studies
- Wnt/β-catenin signaling in development and cancer
- Infectious Diseases and Mycology
Lawrence Berkeley National Laboratory
2020-2024
Joint Genome Institute
2016-2024
United States Department of Energy
2018-2024
University of California, San Francisco
1989-2022
Lawrence Livermore National Laboratory
2010
Rockefeller University
1988
A cDNA clone of murine macrophage inflammatory protein 2 (MIP-2) has been isolated from a library prepared lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and the nucleotide sequence determined. This was used to cDNAs for human homologues MIP-2 phorbol myristate acetate-treated LPS-stimulated U937 cells. Two were sequenced. Human alpha beta are highly homologous each other previously gene, gro/melanoma growth-stimulating activity (MGSA). These three genes, alpha, beta, gro/MGSA,...
A number of macrophage-derived mediators have been implicated in the vascular changes inflammation. We recently reported isolation a novel monokine, macrophage inflammatory protein 1 (MIP-1), which causes local responses vivo, and induces superoxide production by neutrophils vitro. Purified native MIP-1 comprises two peptides with very similar physical characteristics. report here resolution into component SDS-hydroxylapatite chromatography, compare NH2-terminal sequences peptides, now...
Abstract The roots of Arabidopsis thaliana host diverse fungal communities that affect plant health and disease states. Here, we sequence the genomes 41 isolates representative A. root mycobiota for comparative analysis with other 79 plant-associated fungi. Our analyses indicate members evolved from ancestors lifestyles retain large repertoires cell wall-degrading enzymes (PCWDEs) effector-like small secreted proteins. We identify a set 84 gene families associated endophytism, including...
Journal Article The cDNA and derived amino acid sequence for human osteopontin Get access Michael C. Kiefer, Kiefer Chiron Corporation4560 Horton Street, Emeryville, CA 94680, USA Search other works by this author on: Oxford Academic PubMed Google Scholar Diane M. Bauer, Bauer Philip J. Barr Nucleic Acids Research, Volume 17, Issue 8, 25 April 1989, Page 3306, https://doi.org/10.1093/nar/17.8.3306 Published: 1989 history Received: 19 January
We have isolated four insulin-like growth factor binding proteins (IGFBPs) from adult human serum by (IGF) I affinity chromatography and high performance liquid chromatography. A 36-kDa protein (BP), not digestible with N-glycanase, is increased in patients extrapancreatic tumor hypoglycemia during IGF administration healthy adults. Its 38 NH2-terminal amino acids are identical to those of an IGFBP sequence derived a cDNA that cross-hybridizes the rat IGFBP-2 cDNA. With probes encoding...
Corynespora cassiicola is an Ascomycetes fungus with a broad host range and diverse life styles. Mostly known as necrotrophic plant pathogen, it has also been associated rare cases of human infection. In the rubber tree, this causes leaf fall (CLF) disease, which increasingly affects natural production in Asia Africa. It found endophyte South American plantations where no CLF outbreak yet occurred. The C. species genetically highly diverse, but clear relationship evidenced between...
Currents are unique drivers of oceanic phylogeography and thus determine the distribution marine coastal species, along with past glaciations sea-level changes. Here we reconstruct worldwide colonization history eelgrass (Zostera marina L.), most widely distributed flowering plant or seagrass from its origin in Northwest Pacific, based on nuclear chloroplast genomes. We identified two divergent Pacific clades evidence for admixture East coast. Two west-to-east (trans-Pacific) events support...
Abstract Background Colletotrichum fungi infect a wide diversity of monocot and dicot hosts, causing diseases on almost all economically important plants worldwide. is also suitable model for studying gene family evolution fine scale to uncover events in the genome associated with biological changes. Results Here we present sequences 30 species covering within genus. Evolutionary analyses revealed that ancestor diverged late Cretaceous parallel diversification flowering plants. We provide...
The nucleotide sequence for human myelin-associated glycoprotein (MAG) and its deduced amino acid sequence, obtained by analysis of two overlapping cDNA clones isolated from a brain library, is presented compared to that reported rat MAG. provides an open reading frame 1,878 nucleotides encoding peptide 626 acids with calculated molecular weight 69.1 kD. It 89% homologous in the large isoform MAG, 95% homology sequence. contains 9 potential glycosylation sites, one more than rat, shares...
Journal Article The cDNA and derived amino acid sequences of human bovine bone Gla protein Get access Michael C. Kiefer, Kiefer Chiron Corporation4560 Horton Street, Emeryville, CA 94608, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Andrew C.S. Saphire, Saphire Diane M. Bauer, Bauer Philip J. Barr Nucleic Acids Research, Volume 18, Issue 7, 11 April 1990, Page 1909, https://doi.org/10.1093/nar/18.7.1909 Published: 1990 history Accepted: 12 February
Summary Ectomycorrhizal (ECM) fungi are integral to boreal and temperate forest ecosystem functioning nutrient cycling. ECM fungi, however, originate from diverse saprotrophic lineages the impacts of genetic variation across species, especially within a given on function interactions with environment is not well understood. Here, we explore extent intra‐species between four isolates fungus Pisolithus microcarpus , in terms gene regulation, carbon metabolism growth, host, Eucalyptus grandis ....
Efficient utilization of agro-industrial waste, such as sugar beet pulp, is crucial for the bio-based economy. The fungus Aspergillus niger possesses a wide array enzymes that degrade complex plant biomass substrates, and several regulators have been reported to play role in their production. GaaR, AraR, RhaR pectin degradation has previously reported. However, genetic regulation pulp not assessed detail. In this study, we generated set single combinatorial deletion mutants targeting...
Fungal effectors play critical roles in manipulating plant immune responses and promoting colonization. Sphaerulina musiva is a heterothallic ascomycete fungus that causes Septoria leaf spot stem canker disease poplar (Populus spp.) plantations. This can result premature defoliation, branch breakage, increased mortality, plantation failure. However, little known about the interaction between S. poplar. Previous work predicted 142 candidate secreted effector proteins (SmCSEPs), 19 of which...
Enzymatic plant biomass degradation by fungi is a highly complex process and one of the leading challenges in developing biobased economy. Some industrial (e.g. Aspergillus niger) have long history use with respect to for that reason become 'model' species this topic. A. niger major enzyme producer has broad ability degrade based polysaccharides. wild-type, (hemi-)cellulolytic regulator (xlnR) xylulokinase (xkiA1) mutant strains were grown on monocot (corn stover, CS) dicot (soybean hulls,...
Single-cell sequencing of environmental microorganisms is an essential component the microbial ecology toolkit. However, large-scale targeted single-cell for whole-genome recovery uncultivated eukaryotes lagging. The key challenges are low abundance in communities, large complex genomes, and cell walls that difficult to break. We describe a pipeline composed state-of-the art genomics tools protocols optimized poorly studied eukaryotic found at abundance. This consists seven distinct steps,...
ABSTRACT Colletotrichum fungi infect a wide diversity of monocot and eudicot hosts, causing plant diseases on almost all economically important crops worldwide. In addition to its economic impact, is suitable model for the study gene family evolution fine scale uncover events in genome that are associated with biological characters host interactions. Here we present sequences 30 species, 18 them newly sequenced, covering taxonomic within genus. A time-calibrated tree revealed ancestor...
We describe the molecular characterization of a novel human gene on chromosome 1q23.3, termed KAT, which is highly conserved among mammals. The KAT spans genomic region approximately 1.6 kilobases and consists 4 exons encoding 115 amino acid protein with mass about 12.5 kDa. expressed in several tissues, including kidney, liver, skeletal muscle, heart, colon, thymus, spleen, placenta lung. identified an alternatively spliced form, lacking exon 2, mouse tissues. In silico analysis sequence...
The evolution of gene expression is thought to be an important mechanism local adaptation and ecological speciation. Gene divergence occurs through the cis- polymorphisms more widespread effects driven by trans-regulatory factors. Here, we explore sequence in a large sample Panicum hallii accessions encompassing species range using reciprocal transplantation experiment. We observed genotype transplant site drivers divergence, with limited number genes exhibiting genotype-by-site...
Filamentous fungi are of great importance in natural environments because they producers several carbohydrate-active enzymes (CAZymes) that capable degrading the plant cell wall and thus contributing to global carbon cycle. Furthermore, degradation polysaccharides releases sugar monomers through a series enzymatic reactions metabolized as source energy. The large number can be found most diverse contributes genetic variability, reflecting wide diversity being produced. In this study we...
Lignocellulose, the Earth's most abundant biopolymer, is degraded by wood-decaying fungi, specifically white rot fungi (WRF) and brown (BRF), which use different strategies. This study examines expression profiles of AA9 CDH enzymes three WRF species (Heterobasidion annosum, Phanerochaete chrysosporium, Pleurotus ostreatus) two BRF (Fomitopsis pinicola Rhodonia placenta) from Agaricomycetes class, grown on poplar wood or glucose as sole carbon source. Mycelia were collected between days 10...
Abstract Currents are unique drivers of oceanic phylogeography and so determine the distribution marine coastal species, along with past glaciations sea level changes. Here, we reconstruct worldwide colonization history eelgrass ( Zostera marina L.), most widely distributed flowering plant or seagrass from its origin in Northwest Pacific, based on nuclear chloroplast genomes. We identified two divergent Pacific clades evidence for admixture East coast. Multiple west to east (trans-Pacific)...