A5071566189- Plant tissue culture and regeneration
- Bioenergy crop production and management
- Plant Reproductive Biology
- Reproductive Biology and Fertility
- Seed Germination and Physiology
- Plant Pathogens and Fungal Diseases
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant Water Relations and Carbon Dynamics
- Forest ecology and management
- Plant-Microbe Interactions and Immunity
- Microbial Metabolic Engineering and Bioproduction
- Plant Disease Resistance and Genetics
- Nuts composition and effects
- Plant Taxonomy and Phylogenetics
- Biofuel production and bioconversion
- Plant biochemistry and biosynthesis
- Plant Physiology and Cultivation Studies
- Tree Root and Stability Studies
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Mycotoxins in Agriculture and Food
- Ion Transport and Channel Regulation
- Horticultural and Viticultural Research
- Magnetic and Electromagnetic Effects
Université Clermont Auvergne
2014-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2018-2025
Clermont Université
2013-2024
Physique et Physiologie Intégratives de l'Arbre en Environnement Fluctuant
2012-2023
Centre National de la Propriété Forestière
2005-2014
Centre National de la Recherche Scientifique
2012
Institut für Holztechnologie Dresden
2012
Universität Hamburg
2012
Arbres et Réponses aux Contraintes Hydriques et Environnementales
2012
Université Toulouse III - Paul Sabatier
2012
Summary Maritime pine ( P inus pinaster A it.) is a widely distributed conifer species in S outhwestern E urope and one of the most advanced models for research. In current work, comprehensive characterization maritime transcriptome was performed using combination two different next‐generation sequencing platforms, 454 I llumina. De novo assembly provided catalogue 26 020 unique transcripts trees collection 9641 full‐length c DNA s. Quality validated by RT ‐ PCR amplification selected...
A novel category of major intrinsic proteins which share weak similarities with previously identified aquaporin subfamilies was recently in land plants, and named X (for unrecognized) (XIPs). Because XIPs are still ranked as uncharacterized proteins, their further molecular characterization is required. Herein, a systematic fine-scale analysis XIP sequences found flowering plant databases revealed that at least five groups. The phylogenetic relationship these groups the organization...
Abstract The comprehension of metal homeostasis in plants requires the identification molecular markers linked to stress tolerance. Proteomic changes leaves and cambial zone Populus tremula × P. alba (717‐1B4 genotype) were analyzed after 61 days exposure cadmium (Cd) 360 mg/kg soil dry weight pot‐soil cultures. treatment led an acute Cd with a reduction growth photosynthesis. induced display 120 spots for leaf tissue 153 zone. It involved reduced photosynthesis, resulting profound...
A global DNA methylation and proteomics approach was used to investigate somatic embryo maturation in hybrid larch. Each developmental step during embryogenesis associated with a distinct significantly different level: from 45.8% mC for undifferentiated embryos (1-week proliferation) 61.5% immature maturation), while decrease 53.4% mature cotyledonary (8-weeks maturation). The presence of 5-azacytidine (hypo-methylating agent) or hydroxyurea (hyper-methylating the medium altered status...
Corynespora cassiicola is an Ascomycetes fungus with a broad host range and diverse life styles. Mostly known as necrotrophic plant pathogen, it has also been associated rare cases of human infection. In the rubber tree, this causes leaf fall (CLF) disease, which increasingly affects natural production in Asia Africa. It found endophyte South American plantations where no CLF outbreak yet occurred. The C. species genetically highly diverse, but clear relationship evidenced between...
Abstract Background The availability of a large expressed sequence tags (EST) resource and recent advances in high-throughput genotyping technology have made it possible to develop highly multiplexed SNP arrays for multi-objective genetic applications, including the construction meiotic maps. Such approaches are particularly useful species with genome size, precluding use whole-genome shotgun assembly current technologies. Results In this study, 12 k-SNP array was developed maritime pine...
Maritime pine somatic embryos (SEs) require a reduction in water availability (high gellan gum concentration the maturation medium) to reach cotyledonary stage. This key switch, reported specifically for species, is not yet well understood. To facilitate use of embryogenesis mass propagation conifers, we need better understanding embryo development. Comparison both transcriptome (Illumina RNA sequencing) and proteome [two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis...
Abstract Wheat responses to F. graminearum result in a deep and sharp reprogramming of wide range biological processes, including energy‐associated functions related metabolisms. Although these impacts have been thoroughly described at the molecular scale through proteomics transcriptomics studies, phenotypic studies are still needed fill gap between observed events actual disease on ecophysiological processes. Taking advantage gas exchange method, effects two strains contrasting...
Somatic embryos of hybrid larch (Larix×leptoeuropaea) which had been matured for 4 weeks on maturation medium, developed normally medium supplemented with 60 μM ABA, but abnormally no ABA. A comparative structural and histochemical investigation was carried out these two types mature embryos. At the light microscope level, differences between both treatments were visible only after 2–3 maturation. around this time, abnormal development becomes evident macroscopically: ABA-minus remain rather...
Beneficial microorganisms, including members of the Trichoderma genus, are known for their ability to promote plant growth and disease resistance, as well being alternatives synthetic inputs in agriculture. In this study, 111 strains were isolated from rhizospheric soil Florence Aurore, an ancient wheat variety that was cultivated organic farming system Tunisia. A preliminary ITS analysis allowed us cluster these isolates into three main groups, T. harzianum (74 isolates), lixii (16...
Leaf conductance, water relations, growth, and abscisic acid (ABA) concentrations in xylem sap, root apices leaves were assessed oak seedlings (Quercus robur L.) grown with a system divided between two compartments subjected to one of four treatments: (a) well watered, WW; (b) half exposed soil drying kept WD; (c) whole drought, DD; (d) severed, RE. Sharp decreases plant stomatal leaf potential, hydraulic conductance growth observed during DD treatment. No significant differences potential...
An indirect phenotyping method was developed in order to estimate the susceptibility of rubber tree clonal varieties Corynespora Leaf Fall (CLF) disease caused by ascomycete cassiicola. This consists quantifying impact fungal exudates on detached leaves measuring induced electrolyte leakage (EL%). The tested were either crude culture filtrates from diverse C. cassiicola isolates or purified cassiicolin (Cas1), a small secreted effector protein produced aggressive isolate CCP. test found be...
An integrated physiological and proteomic approach was used to investigate the effects of high gellan gum concentration in medium during maturation somatic embryos (SE) hybrid larch, by comparing incubated media with a (8 g l(-1) ) standard (4 after 1, 3, 6 8 weeks maturation. Because reduced availability water medium, cultured had lower osmotic potential (Ψπ) contents, but higher dry weights (DWs), at compared on medium. The induced desiccation that is characteristic zygotic embryo Total...
Fusarium graminearum, the main causal agent of Head Blight (FHB), is one most damaging pathogens in wheat. Because complex organization wheat resistance to FHB, this pathosystem represents a relevant model elucidate molecular mechanisms underlying plant susceptibility and identify their drivers, pathogen's effectors. Although F. graminearum catalog effectors has been well characterized at genome scale, planta studies are needed confirm effective accumulation host tissues role during...
The effects of cadmium and zinc on growth mineral distribution in Populus tremula × P. alba genotype 717-1B4 were investigated. Exposure to 360 mg(Cd) kg-1(soil) resulted accumulation Cd all organs inhibition primary secondary as well the net photosynthetic rate. No occurred under exposure. was mainly stored woody parts stem, whereas preferentially localized leaves. treatment also altered Zn2+, Ca2+, Mg2+, K+, Fe2+ different organs.
Somatic embryogenesis techniques have been developed for most coniferous species, but only using very juvenile material. To extend the techniques' scope, better integrated understanding of key biological, physiological and molecular characteristics embryogenic state is required. Therefore, embryonal masses (EMs) non-embryogenic calli (NECs) compared during proliferation at multiple levels. EMs NECs originating from a single somatic embryo (isogenic lines) each three unrelated genotypes were...
Cellular aquaporin water channels (AQPs) constitute a large family of transmembrane proteins present throughout all kingdoms life, playing important roles in the uptake and many solutes across membranes. In olive trees, AQP diversity, protein features their biological functions are still largely unknown. This study focuses on structure functional evolution diversity subfamilies two wild species Olea europaea var. sylvestris (OeuAQPs) domesticated cv. Picual (OleurAQPs), describes involvement...
Aquaporins are transmembrane water channels found in almost every living organism. Numerous studies have brought a good understanding of both transport through their pores and the regulations taking place at molecular level, but subtleties remain to be clarified. Recently, voltage-related gating mechanism involving conserved arginine channel's main constriction was captured for human aquaporins dynamics studies. With similar approach, we show that this voltage-gating could among family...
— Thirty-to 40-year-old oak trees (Quercus petraea Matt Liebl and Q robur L) growing in a forest stand near Champenoux (Nancy, France) were submitted to an imposed drought lysimeter during two successive summers.Xylem sap was extracted from leafy twigs of per species at regular intervals the onset drought, controls parallel.Predawn leaf water potential, soil potential different depths midday stomatal conductance sun-exposed leaves recorded same pace.Concentrations abscisic acid (ABA)...
Embryogenic tissue of hybrid larch (Larix× marschlinsii Coaz) was multiplied on Medium M (modified MSG medium supplemented with the plant growth regulators (PGRs) 2,4-dichlorophenoxyacetic acid (2,4-D; 9 μM) and N-6-benzyladenine (2.25 μM)). After 1 week, cultures were transferred to either lacking PGRs (Medium C–) or but 1% activated charcoal C+). Embryos sampled after week M, C– C+. analyzed by ELISA for abscisic (ABA), acid–glucose ester, 2,4-D, indole-3-acetic (IAA), indole-3-aspartate...