A5015696449- Biofuel production and bioconversion
- Bioenergy crop production and management
- Plant Gene Expression Analysis
- Lignin and Wood Chemistry
- Tree Root and Stability Studies
- Forest ecology and management
- Plant Water Relations and Carbon Dynamics
- Genetically Modified Organisms Research
- Plant tissue culture and regeneration
- Insect Resistance and Genetics
- Wood Treatment and Properties
- Plant Molecular Biology Research
- Transgenic Plants and Applications
- Horticultural and Viticultural Research
- Animal Genetics and Reproduction
- Microbial Metabolic Engineering and Bioproduction
- Enzyme-mediated dye degradation
- Genomics and Phylogenetic Studies
- Forest Biomass Utilization and Management
- Forest Insect Ecology and Management
- Polysaccharides and Plant Cell Walls
- Biochemical and biochemical processes
- CRISPR and Genetic Engineering
- Plant Reproductive Biology
- Bioinformatics and Genomic Networks
UMR BIOdiversity, GEnes & Communities
2016-2024
Université de Bordeaux
2019-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Centre d'Études Scientifiques et Techniques d'Aquitaine
2016-2023
Biologie intégrée pour la valorisation de la diversité des arbres et de la forêt
2018
Centre National de la Propriété Forestière
2008-2017
Institut National de la Recherche Agronomique
1999-2015
Ghent University
1993-2007
Vlaams Instituut voor Biotechnologie
1996-2007
Oak Ridge National Laboratory
2006
We report the draft genome of black cottonwood tree, Populus trichocarpa . Integration shotgun sequence assembly with genetic mapping enabled chromosome-scale reconstruction genome. More than 45,000 putative protein-coding genes were identified. Analysis assembled revealed a whole-genome duplication event; about 8000 pairs duplicated from that event survived in A second, older is indistinguishably coincident divergence and Arabidopsis lineages. Nucleotide substitution, tandem gene...
Oaks are an important part of our natural and cultural heritage. Not only they ubiquitous in most common landscapes1 but have also supplied human societies with invaluable services, including food shelter, since prehistoric times2. With 450 species spread throughout Asia, Europe America3, oaks constitute a critical global renewable resource. The longevity (several hundred years) probably underlies their emblematic historical importance. Such long-lived sessile organisms must persist the face...
Abstract Cinnamoyl-CoA reductase (CCR) catalyzes the penultimate step in monolignol biosynthesis. We show that downregulation of CCR transgenic poplar (Populus tremula × Populus alba) was associated with up to 50% reduced lignin content and an orange-brown, often patchy, coloration outer xylem. Thioacidolysis, nuclear magnetic resonance (NMR), immunocytochemistry epitopes, oligolignol profiling indicated relatively more syringyl than guaiacyl units. The cohesion walls affected, particularly...
We evaluated lignin profiles and pulping performances of 2-year-old transgenic poplar (Populus tremula x Populus alba) lines severely altered in the expression caffeic acid/5-hydroxyferulic acid O-methyltransferase (COMT) or cinnamyl alcohol dehydrogenase (CAD). Transgenic poplars with CAD COMT antisense constructs showed growth similar to control trees. down-regulated displayed a red coloration mainly outer xylem. A 90% lower activity did not change content but dramatically increased...
Summary Lignin is a polymeric constituent of the cell wall that needs to be removed during paper making process. Bi‐specific caffeic acid/5‐hydroxyferulic acid O ‐methyltransferase (COMT) catalyses ‐methylation and 5‐hydroxyferulic ferulic sinapic acid, respectively. These compounds are intermediates in biosynthesis lignin precursors. Therefore, COMTs potential target enzymes for reducing amount, or modifying composition, plants. Different antisense sense constructs have been expressed gene...
• Fifteen poplar cDNA encoding fasciclin-like arabinogalactan proteins (PopFLAs) were finely characterized, whereas the presence of (AGPs) was globally assessed during wood formation. PopFLAs transcript accumulation analysed through EST distribution in libraries, semi-quantitative RT-PCR, microarray experiment and Northern blot analysis. Similarly, AGPs contents quantified by rocket electrophoresis. further examined Western blotting immunocytolocalization. Ten specifically expressed tension...
Significance In the transition from a fossil-based to bio-based economy, bioethanol will be generated lignocellulosic biomass of second-generation biofuel crops, such as poplar. The lignin polymers in plant cell walls represent main factor determining recalcitrance enzymatic processing. We have grown genetically modified poplars, down-regulated for cinnamoyl-CoA reductase (CCR), an enzyme biosynthetic pathway, field trials Belgium and France. show that wood samples derived transgenic trees...
Abstract The 1.5 Gbp/2C genome of pedunculate oak ( Quercus robur ) has been sequenced. A strategy was established for dealing with the challenges imposed by sequencing such a large, complex and highly heterozygous whole‐genome shotgun WGS approach, without use costly time‐consuming methods, as fosmid or BAC clone‐based hierarchical methods. combined short long reads. Over 49 million reads provided Roche 454 GS ‐ FLX technology were assembled into contigs shorter Illumina sequence from...
This review highlights some of the discoveries and applications made possible by "omics" technologies over last 10 years provides perspectives for pioneering research to increase our understanding tree biology. A decade after first forest genome sequence was released into public domain, rapidly evolving genomics bioinformatics toolbox has advanced structure, functioning, evolution genomes. that have trees past years. In this review, we start current intricacies gene regulation reproduction,...
Abstract Purpose of Review In this review, we synthesise current knowledge on trade-offs among traits in key fitness dimensions and identify major research gaps with the intention laying groundwork for a rapid advance tree breeding multiple objectives as contribution to sustainability planted forests future. Recent Findings Trade-offs growth, reproduction, defence, stress tolerance product quality predicted theoretically have been reported experimentally many programmes. Among these...
Summary A molecular marker compound, derived from lignin by the thioacidolysis degradative method, for structures produced when ferulic acid is incorporated into in angiosperms (poplar, Arabidopsis, tobacco), has been structurally identified as 1,2,2‐trithioethyl ethylguaiacol [1‐(4‐hydroxy‐3‐methoxyphenyl)‐1,2,2‐tris(ethylthio)ethane]. Its truncated side chain and distinctive oxidation state suggest that it derives undergone bis ‐8‐ O‐ 4 (cross) coupling during lignification, validated...
Abstract Background In temperate regions, the time lag between vegetative bud burst and set determines duration of growing season trees (i.e. wood biomass production). Dormancy, period during which plant is not growing, allows to avoid cold injury resulting from exposure low temperatures. An understanding molecular machinery controlling shift these two phenological states key importance in context climatic change. The objective this study was identify genes upregulated endo- ecodormancy,...
Complete Populus genome sequences are available for the nucleus (P. trichocarpa; section Tacamahaca) and chloroplasts (seven species), but not mitochondria. Here, we provide complete of chloroplast mitochondrion clones P. tremula W52 x alba 717-1B4 (section Populus). The organization genomes both is described. A phylogenetic tree constructed from all DNA was congruent with assignment related species to different sections. In total, 3,024 variable nucleotide positions were identified among...
Abstract The pace of tree microevolution during Anthropocene warming is largely unknown. We used a retrospective approach to monitor genomic changes in oak trees since the Little Ice Age (LIA). Allelic frequency were assessed from whole-genome pooled sequences for four age-structured cohorts sessile (Quercus petraea) dating back 1680, each three different forests France. genetic covariances allelic increased between successive time periods, highlighting genome-wide effects linked selection....
Abstract: Xylogenesis involves successive developmental processes ‐ cambial division, cell expansion and differentiation, death each occurring along a gradient from the cambium to pith of stem. Taking advantage high level organisation wood tissues, we isolated zone (CZ), differentiating xylem (DX) mature (MX) both tension (TW) opposite (OW) bent poplars. Four different cDNA libraries were then constructed used generate 10 062 EST, reflecting genes expressed in tissues. For most abundant...
In Eukaryotes, LIM proteins act as developmental regulators in basic cellular processes such regulating the transcription or organizing cytoskeleton. The domain protein family plants has mainly been studied sunflower and tobacco plants, where several of its members exhibit a specific pattern expression pollen. this paper, we finely characterized poplar six transcripts encoding these proteins. Populus trichocarpa genome, 12 gene models identified all appear to be duplicated genes. addition,...