A5078714342- Mycorrhizal Fungi and Plant Interactions
- Fungal Biology and Applications
- Plant nutrient uptake and metabolism
- Legume Nitrogen Fixing Symbiosis
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- Rabies epidemiology and control
- Virus-based gene therapy research
- Fungal and yeast genetics research
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- Vector-borne infectious diseases
- Polyamine Metabolism and Applications
- Viral Infections and Outbreaks Research
- Mycobacterium research and diagnosis
- Viral Infections and Vectors
- Plant Parasitism and Resistance
- Vector-Borne Animal Diseases
- Yersinia bacterium, plague, ectoparasites research
- Plant-Microbe Interactions and Immunity
- Plant and animal studies
- Mosquito-borne diseases and control
- Virology and Viral Diseases
- Influenza Virus Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
Université de Lorraine
2012-2023
Interactions Arbres-Microorganismes
2012-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2023
Délégation Paris 6
2019
Centro de Investigación en Sanidad Animal
2017
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2017
Institut National de la Recherche Agronomique
1995-2013
Oak Ridge National Laboratory
2006
University of Tennessee at Knoxville
2006
West Virginia University
2006
We report the draft genome of black cottonwood tree, Populus trichocarpa . Integration shotgun sequence assembly with genetic mapping enabled chromosome-scale reconstruction genome. More than 45,000 putative protein-coding genes were identified. Analysis assembled revealed a whole-genome duplication event; about 8000 pairs duplicated from that event survived in A second, older is indistinguishably coincident divergence and Arabidopsis lineages. Nucleotide substitution, tandem gene...
The fungus Laccaria bicolor — seen in its above-ground fruiting body presence as the 'bicoloured deceiver' mushroom lives symbiotically on roots of trees. Its genome has now been sequenced, and key features characterized by transcript profiling. study throws light mechanism mycorrhizal symbiosis, union soil fungi that is vital important to plant productivity. And it will be keen interest evolutionary biologists for revelations about plant–fungus interactions shaping genomes over time....
The genome of the Périgord black truffle Tuber melanosporum Vittad., a gourmet delicacy that grows symbiotically on roots European oak, has been sequenced. This is largest and most complex fungal so far unusually gene-poor, but rich in mobile genetic elements known as transposons. Comparison with sequence Laccaria bicolor, another ectomycorrhizal symbiotic fungus, reveals contrasting gene sets reflecting two different molecular toolkits have evolved independently to fit root symbiont...
Agaricus bisporus is the model fungus for adaptation, persistence, and growth in humic-rich leaf-litter environment. Aside from its ecological role, A. has been an important component of human diet over 200 y worldwide cultivation "button mushroom" forms a multibillion dollar industry. We present two genomes, their gene repertoires transcript profiles on compost during mushroom formation. The genomes encode full repertoire polysaccharide-degrading enzymes similar to that wood-decayers....
Significance Plants use the hormone jasmonic acid (JA) to modulate plant:microbe interactions. Disease-causing microbes proteins alter host JA signaling aid their growth in plant tissues. Beneficial symbiotic fungi, which colonize tissues and provide essential ecosystem services such as carbon sequestration fertilization, can also cells promote colonization. Here, we demonstrate that MiSSP7 (Mycorrhiza-induced small secreted protein-7) protein of beneficial fungus Laccaria bicolor interacts...
Abstract A sequence coding for a peroxiredoxin (Prx) was isolated from xylem/phloem cDNA library Populus trichocarpaand subsequently inserted into an expression plasmid yielding the construction pET-Prx. The recombinant protein produced inEscherichia coli cells and purified to homogeneity with high yield. poplar Prx is composed of 162 residues, property that makes it shortest plant so far. It shown monomeric possesses two conserved cysteines (Cys). degrades hydrogen peroxide alkyl...
• Ammonium and nitrate are the prevalent nitrogen sources for growth development of higher plants. Here, we report on characterization ammonium transporter (AMT) family in perennial species Populus trichocarpa. In silico analysis expression AMT genes from poplar was performed. addition, AMT1;2 AMT1;6 function studied detail by heterologous yeast. The P. trichocarpa genome contains 14 putative AMTs, which is more than twice number AMTs Arabidopsis. roots, high-affinity strongly increased upon...
The coordinated transcriptomic responses of both mutualistic ectomycorrhizal (ECM) fungi and their hosts during the establishment symbiosis are not well-understood. This study characterizes alterations ECM fungus Laccaria bicolor different colonization stages on two (Populus trichocarpa Pseudotsuga menziesii) compares this to variations P. across same time-points. A large number L. genes (≥ 8,000) were significantly regulated at transcriptional level in least one stage colonization. From our...
Abstract Rift V alley fever ( RVF ) is a mosquito‐borne disease caused by the R ift virus RVFV ). affects large number of species, including human, and has severe impact on public health economy, especially in A frican countries. The present study examined vector competence three different E uropean mosquito Culex pipiens L innaeus, 1758) form molestus (Diptera: Culicidae), hybrid Stegomyia albopicta (= Aedes albopictus S kuse, 1894) Culicidae). Mosquitoes were artificially fed with blood...
Within boreal and temperate forest ecosystems, the majority of trees shrubs form beneficial relationships with mutualistic ectomycorrhizal (ECM) fungi that support plant health through increased access to nutrients as well aiding in stress pest tolerance. The intimate interaction between fungal hyphae roots results a new symbiotic “organ” called ECM root tip. Little is understood concerning metabolic reprogramming favors formation this hybrid tissue compatible interactions what prevents tips...
Summary External hyphae, which play a key role in nitrogen nutrition of trees, are considered as the absorbing structures ectomycorrhizal symbiosis. Here, we have cloned and characterized Hebeloma cylindrosporum AMT1 , GLNA GDHA genes, encode third ammonium transporter, glutamine synthetase an NADP‐dependent glutamate dehydrogenase respectively. Amt1 can fully restore pseudohyphal growth defect Saccharomyces cerevisiae mep2 mutant, this is first evidence that heterologous member Mep/Amt...
Ectomycorrhizal interactions established between the root systems of terrestrial plants and hyphae from soil-borne fungi are most ecologically widespread plant symbioses. The efficient uptake a broad range nitrogen (N) compounds by fungal symbiont their further transfer to host is major feature this symbiosis. Nevertheless, we far understand which N form preferentially transferred what key molecular determinants required for transfer. Exhaustive in silico analysis N-compound transporter...
The ectomycorrhizal (ECM) symbiosis, a mutualistic plant-fungus association, plays fundamental role in forest ecosystems by enhancing plant growth and providing host protection from root diseases. cellular complexity of the symbiotic organ, characterized differentiation structurally specialized tissues (i.e. fungal mantle Hartig net), is major limitation to study gene expression such specific compartments. We investigated transcriptional landscape ECM fungus Tuber melanosporum during stages...
The gene encoding a superoxide dismutase (PiSOD) was cloned by suppressive subtractive hybridization from cDNA library of the ectomycorrhizal fungus, Paxillus involutus , grown under cadmium‐stress conditions. encoded protein presumed to be localized in peroxisomes because it contained C‐terminal peroxisomal localization peptide (SKL) and lacked an N‐terminal mitochondrial transit peptide. Complementation Escherichia coli SOD null strain that is unable grow presence paraquat or cadmium...
To examine pathways of glutamate and glutamine metabolism in the ectomycorrhizal fungus Paxillus involutus, tracer kinetic experiments were performed using l-[U-14C]glutamate L-[U-14C]glutamine enzyme inhibitors methionine sulfoximine (MSX), azaserine (AZA) aminooxyacetate (AOA). When [14C]glutamate was supplied to fungal cultures, 25% radioactivity amino acid fraction incorporated into after 5 min feeding, but MSX inhibited incorporation 14C by 85%, suggesting rapid operation synthetase....
Heterologous expression of the yeast triple Mep mutant has enabled first molecular characterization AMT/MEP family members in an ectomycorrhizal fungus. External hyphae, which play a key role nitrogen nutrition trees, are considered as absorbing structure symbiosis and therefore studies on ammonium transport hyphae urgently needed. The kinetic properties AMT2 AMT3 from Hebeloma cylindrosporum were studied Saccharomyces cerevisiae . Expression HcAmts restored retention within cells. did not...
ABSTRACT The development of ectomycorrhizal symbiosis leads to drastic changes in gene expression both partners. However, little is known about the spatial regulation symbiosis-regulated genes. Using cDNA array profiling, we compared levels fungal genes corresponding approximately 1,200 expressed sequenced tags root tips (ECM) and connected extraradical mycelium (EM) for Paxillus involutus - Betula pendula association grown on peat a microcosm system. Sixty-five unique were found be...
SUMMARY Detailed examination of the structural and functional integration taking place during mycorrhiza formation necessitates rapid aseptic synthesis uniform mycorrhizal structures at well‐defined stages development. A system is described for ectomycorrhizas between Betula pendula Roth, Paxillus iiwolulus (Batsch) Fr. Small sterile seedlings were placed directly on fungal colonies growing cellophane covered agar plates. Microscopic course development indicated an initial colonization roots...
The molecular mechanisms underlying the response of ectomycorrhizal fungi to heavy metals in general and cadmium particular remain poorly understood. We screened 2040 arrayed cDNAs fungus Paxillus involutus identify cadmium-responsive genes by using differential hybridization. Forty nine (2.4%) were differentially expressed, among which transcripts coding a laccase, an aconitase, metallothionein upregulated 3.9-, 3.7- 2.8-fold, respectively, whereas hydrophobins threonine dehydratase...
Glutamine synthetase (GS) and NADP-dependent glutamate dehydrogenase (NADP-GDH) play a key role in nitrogen assimilation the ectomycorrhizal fungus Laccaria laccata (Scop. ex Fr. Cke) strain S 238. The two enzymes were purified to apparent electrophoretic homogeneity by three-step procedure involving diethylaminoethyl (DEAE)-Trisacryl affinity chromatography, DEAE-5PW fast protein liquid chromatography. This purification scheme resulted 23 62% recovery of initial activity for GS NADP-GDH,...