A5091800272- Fungal Biology and Applications
- Microbial Natural Products and Biosynthesis
- Plant Pathogens and Fungal Diseases
- Fungal and yeast genetics research
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Mycorrhizal Fungi and Plant Interactions
- Plant biochemistry and biosynthesis
- Plant tissue culture and regeneration
- Veterinary medicine and infectious diseases
- Plant Disease Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Fungal Plant Pathogen Control
- Transgenic Plants and Applications
- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- Plant and Fungal Interactions Research
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Insect symbiosis and bacterial influences
- Bacillus and Francisella bacterial research
- Nematode management and characterization studies
- Toxin Mechanisms and Immunotoxins
- Salmonella and Campylobacter epidemiology
- Pharmacological Effects of Natural Compounds
University of Bristol
2016-2025
Winston-Salem State University
2025
National Research Institute for Earth Science and Disaster Resilience
2023
Ghent University
2023
UCLouvain
2023
Wageningen University & Research
2023
Royal Observatory of Belgium
2023
Tyndall Centre
2018-2019
Sulaimani Polytechnic University
2019
Sheffield Teaching Hospitals NHS Foundation Trust
2017
The plant-pathogenic fungus Mycosphaerella graminicola (asexual stage: Septoria tritici) causes septoria tritici blotch, a disease that greatly reduces the yield and quality of wheat. This is economically important in most wheat-growing areas worldwide threatens global food production. Control has been hampered by limited understanding genetic biochemical bases pathogenicity, including mechanisms infection resistance host. Unlike other plant pathogens, M. long latent period during which it...
Agaricus bisporus is the model fungus for adaptation, persistence, and growth in humic-rich leaf-litter environment. Aside from its ecological role, A. has been an important component of human diet over 200 y worldwide cultivation "button mushroom" forms a multibillion dollar industry. We present two genomes, their gene repertoires transcript profiles on compost during mushroom formation. The genomes encode full repertoire polysaccharide-degrading enzymes similar to that wood-decayers....
Genomic DNA from the insect pathogenic fungus Beauveria bassiana was used as a template in PCR with degenerate primers designed to amplify fragment of C-methyl transferase (CMeT) domain highly reduced fungal polyketide synthase (PKS). The resulting 270-bp product homologous other PKS CMeT domains and probe isolate 7.3-kb genomic BamH1 library. Further library probing TAIL-PCR then gave 21.9-kb contig that encoded 12.9-kb fused type I PKS-NRPS ORF together ORFs encoding oxidative reductive...
A gene cluster encoding the biosynthesis of fungal tropolone stipitatic acid was discovered in Talaromyces stipitatus (Penicillium stipitatum) and investigated by targeted knockout. minimum three genes are required to form nucleus: tropA encodes a nonreducing polyketide synthase which releases 3-methylorcinaldehyde; tropB FAD-dependent monooxygenase dearomatizes 3-methylorcinaldehyde via hydroxylation at C-3; tropC non-heme Fe(II)-dependent dioxygenase catalyzes oxidative ring expansion...
The mechanism of programming iterative highly reducing polyketide synthases remains one the key unsolved problems secondary metabolism. We conducted rational domain swaps between encoding biosynthesis closely related compounds tenellin and desmethylbassianin. Expression hybrid synthetases in Aspergillus oryzae led to production reprogrammed which changes methylation pattern chain length could be mapped swaps. These experiments reveal for first time origin these systems. Domain combined with...
Fungi are often inconspicuous in nature and this means it is all too easy to overlook their importance.Often referred as the "Forgotten Kingdom", fungi key components of life on planet.The phylum Basidiomycota, considered contain most complex evolutionarily advanced members Kingdom, includes some iconic fungal species such gilled mushrooms, puffballs bracket fungi.Basidiomycetes inhabit a wide range ecological niches, carrying out vital ecosystem roles, particularly carbon cycling symbiotic...
Abstract Semi-synthetic derivatives of the tricyclic diterpene antibiotic pleuromutilin from basidiomycete Clitopilus passeckerianus are important in combatting bacterial infections human and veterinary medicine. These compounds belong to only new class antibiotics for applications, with novel mode action lack cross-resistance, representing a great potential. Basidiomycete fungi, being dikaryotic, not generally amenable strain improvement. We report identification seven-gene gene cluster...
Abstract Strobilurins from fungi are the inspiration for creation of β-methoxyacrylate class agricultural fungicides. However, molecular details biosynthesis strobilurins have remained cryptic. Here we report sequence genomes two that produce and show each contains a biosynthetic gene cluster, which encodes highly reducing polyketide synthase with very unusual C-terminal hydrolase methyltransferase domains. Expression stpks1 in Aspergillus oryzae leads to production prestrobilurin A when...
Abstract The tenS gene encoding tenellin synthetase (TENS), a 4239‐residue polyketide synthase nonribosomal‐peptide (PKS‐NRPS) from Beauveria bassiana , was expressed in Aspergillus oryzae M‐2‐3. This led to the production of three new compounds, identified as acyl tetramic acids, and numerous minor metabolites. Consideration structures these compounds indicates that putative C‐terminal thiolester reductase (R) domain does not act reductase, but appears Dieckmann cyclase (DKC). Expression...
Late stage oxidations during the biosynthesis of 2-pyridone tenellin in insect pathogenic fungus Beauveria bassiana were investigated by a combination gene knockout, antisense RNA, and coexpression studies. Open reading frames (ORF) 3 4 biosynthetic cluster previously shown to encode trans-acting enoyl reductase hybrid polyketide synthase nonribosomal peptide synthetase (PKS-NRPS), respectively, which together synthesize acyltetramic acid pretenellin-A. In this work, we have that ORF1...
Salmonella enterica serovar Typhimurium SL1344, in which efflux pump genes (acrB, acrD, acrF, tolC) or regulatory thereof (marA, soxS, ramA) were inactivated, was grown the presence of 240 antimicrobial and nonantimicrobial agents Biolog Phenotype MicroArray. Mutants lacking tolC, acrB, ramA grew significantly worse than other mutants 48 (some have not previously been identified as substrates AcrAB-TolC) particularly poorly phenothiazines, are human antipsychotics. MIC testing revealed that...
The mechanisms by which RND pumps contribute to pathogenicity are currently not understood. Using the AcrAB-TolC system as a paradigm multidrug-resistant efflux pump and Salmonella enterica serovar Typhimurium model pathogen, we have demonstrated that AcrA, AcrB, TolC each required for efficient adhesion invasion of epithelial cells macrophages in vitro. In addition, AcrB necessary colonize poultry. Mutants lacking acrA, acrB, or tolC showed differential expression major operons proteins...
ABSTRACT The transcriptomes of Salmonella enterica serovar Typhimurium SL1344 lacking a functional ramA or ramR with plasmid-mediated high-level overexpression were compared to those the wild-type parental strain. Inactivation led increased expression 14 SPI-1 genes and decreased three SPI-2 genes, it altered ribosomal biosynthetic several amino acid pathways. Furthermore, disruption survival within RAW 264.7 mouse macrophages attenuation BALB/c ByJ model. Highly overexpressed encoding...
Isolation and sequencing of a PKS gene isolated from xenovulene-producing cultures Acremonium strictum indicated the presence NT-, KS-, AT-, PT-, C-MeT- R-domains; heterologous expression in Aspergillus oryzae resulted production 3-methylorcinaldehyde, demonstrating role terminal reductase domain product release.
Fungal chemical factory: Controlled expression of the complete tenellin biosynthetic pathway from Beauveria bassiana was achieved in heterologous host Aspergillus oryzae by replacing promoters all four its component genes with A. amyB promoter; this produces at over five times concentration native host.
Heterologous expression of key components the <italic>Magnaporthe grisea ACE1</italic> gene cluster produces a potential precursor cryptic avirulence signalling compounds that induce resistance to <italic>M. grisea</italic> in rice.
Abstract The rise in antibiotic resistance is a major threat for human health. Basidiomycete fungi represent an untapped source of underexploited antimicrobials, with pleuromutilin—a diterpene produced by Clitopilus passeckerianus —being the only from these leading to commercial derivatives. Here we report genetic characterisation steps involved pleuromutilin biosynthesis, through rational heterologous expression Aspergillus oryzae coupled isolation and detailed structural elucidation...
Biosynthetic gene clusters encoding the production of squalestatin S1 have been discovered and exploited to produce new analogs.