A5067445038- Banana Cultivation and Research
- Nematode management and characterization studies
- Mycotoxins in Agriculture and Food
- Plant Pathogens and Fungal Diseases
- Genomics and Phylogenetic Studies
- Biotechnology and Related Fields
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Insect Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Protein Structure and Dynamics
- Fungal Infections and Studies
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Plant Disease Resistance and Genetics
- Genetically Modified Organisms Research
- Insect-Plant Interactions and Control
- Plant tissue culture and regeneration
- Neurobiology and Insect Physiology Research
- Biochemical and Structural Characterization
- Enzyme Structure and Function
- Insect and Arachnid Ecology and Behavior
- Antifungal resistance and susceptibility
- Genetics, Bioinformatics, and Biomedical Research
- Coffee research and impacts
Brazilian Agricultural Research Corporation
2012-2024
Agricultural Research Corporation
2005-2007
Universidade de Brasília
2002
Universidade Federal de Minas Gerais
1999
The plant-pathogenic fungus Mycosphaerella graminicola (asexual stage: Septoria tritici) causes septoria tritici blotch, a disease that greatly reduces the yield and quality of wheat. This is economically important in most wheat-growing areas worldwide threatens global food production. Control has been hampered by limited understanding genetic biochemical bases pathogenicity, including mechanisms infection resistance host. Unlike other plant pathogens, M. long latent period during which it...
Abstract Background Due to its origin, peanut has a very narrow genetic background. Wild relatives can be source of variability for cultivated peanut. In this study, the transcriptome wild species Arachis stenosperma accession V10309 was analyzed. Results ESTs were produced from four cDNA libraries RNAs extracted leaves and roots A. . Randomly selected clones sequenced generate 8,785 ESTs, which 6,264 (71.3%) had high quality, with 3,500 clusters: 963 contigs 2537 singlets. Only 55.9%...
The prevalence of invasive fungal infections (IFIs) has increased steadily worldwide in the last few decades. Particularly, there been a global rise number among immunosuppressed people. These patients present severe clinical forms infections, which are commonly fatal, and they more susceptible to opportunistic than non-immunocompromised IFIs have historically associated with high morbidity mortality, partly because limitations available antifungal therapies, including side effects,...
Many commercial banana varieties lack sources of resistance to pests and diseases, as a consequence sterility narrow genetic background. Fertile wild relatives, by contrast, possess greater variability represent potential disease genes (R-genes). The largest known family plant R-genes encode proteins with nucleotide-binding site (NBS) C-terminal leucine-rich repeat (LRR) domains. Conserved motifs in such diverse species offer means for isolation candidate which may be involved defence. A...
We have demonstrated that the 80 kDa POP Tc80 (prolyl oligopeptidase of Trypanosoma cruzi) is involved in process cell invasion, since specific inhibitors block parasite entry into non-phagocytic mammalian host cells. In contrast with other POPs, capable hydrolysing large substrates, such as fibronectin and native collagen. this study, we present cloning POPTc80 gene, whose deduced amino acid sequence shares considerable identity members family, mainly within its C-terminal portion forms...
Abstract Background Although banana ( Musa sp.) is an important edible crop, contributing towards poverty alleviation and food security, limited transcriptome datasets are available for use in accelerated molecular-based breeding this genus. 454 GS-FLX Titanium technology was employed to determine the sequence of gene transcripts genotypes acuminata ssp. burmannicoides Calcutta 4 M. subgroup Cavendish cv. Grande Naine, contrasting resistance fungal pathogen Mycosphaerella musicola , causal...
The brown marmorated stink bug, Halyomorpha halys, is a devastating invasive species in the USA. Similar to other insects, olfaction plays an important role its survival and reproduction. As odorant-binding proteins (OBPs) are involved initial semiochemical recognition steps, we used RNA-Sequencing (RNA-Seq) identify OBPs antennae, studied their expression pattern different body parts under stimulation by either aggregation or alarm pheromone food odorants. Thirty full-length putative...
A large-scale mapping of the worker honeybee brain proteome was achieved by MudPIT. We identified 2742 proteins from forager and nurse samples; 17% total were found to be differentially expressed spectral count sampling statistics a G-test. Sequences compared with EuKaryotic Orthologous Groups (KOG) catalog set using BLASTX then categorized into major KOG categories most similar sequences. According this categorization, showed increased expression implicated in translation, ribosomal...
To evaluate the activity of cry1Ia gene against cotton pests, Spodoptera frugiperda and Anthonomus grandis.Had isolated characterized a toxin from Bacillus thuringiensis S1451 strain which have been previously shown to be toxic S. A. grandis. The (cry1Ia) was amplified by PCR, sequenced, cloned into genome baculovirus. Cry1Ia protein expressed in baculovirus infected insect cells, producing inclusions cells. has used bioassays grandis.Bioassays using purified recombinant showed high toxicity...
The prevalence of invasive fungal infections worldwide has increased in the last decades. development specific drugs targeting pathogenic fungi without producing collateral damage to mammalian cells is a daunting pharmacological challenge. Indeed, many toxicities and drug interactions observed with contemporary antifungal therapies can be attributed "nonselective" enzymes or cell membrane systems found host cells. A computer-aided screening strategy against TRR1 protein Paracoccidioides...
Olfaction plays a fundamental role in insect survival through resource location and intra interspecific communications. We used RNA-Seq to analyze transcriptomes for odorant-binding proteins (OBPs) from major stink bug pest species Brazil, Euschistus heros, Chinavia ubica, Dichelops melacanthus, their egg parasitoid, Telenomus podisi. identified 23 OBPs E. 25 C. 9 D. 7 T. The deduced amino acid sequences of the full-length had low intraspecific similarity, but very high similarity between...
Banana (Musa acuminata) is a crop contributing to global food security. Many varieties lack resistance biotic stresses, due sterility and narrow genetic background. The objective of this study was develop an expressed sequence tag (EST) database transcripts during compatible incompatible banana-Mycosphaerella fijiensis (Mf) interactions. Black leaf streak disease (BLSD), caused by Mf, destructive banana. Microsatellite markers were developed as resource for improvement.cDNA libraries...
Tryptophan synthase (TRPS) is a complex enzyme responsible for tryptophan biosynthesis. It occurs in bacteria, plants, and fungi as an αββα heterotetramer. Although encoded by independent genes bacteria fungi, TRPS generated single gene that concurrently expresses the α β entities, which are linked elongated peculiar segment. We conducted 1 µs all-atom molecular dynamics simulations on Hemileia vastatrix to address two questions: (i) role of linker segment (ii) comparative mode action. Since...
The osmotin proteins of several plants display antifungal activity, which can play an important role in plant defense against diseases. Thus, this protein be useful as a source for biotechnological strategies aiming to combat fungal In work, we analyzed the activity cacao osmotin-like (TcOsm1) and two osmotin-derived synthetic peptides with antimicrobial features, differing by five amino acids residues at N-terminus. Antimicrobial tests showed that TcOsm1 expressed Escherichia coli inhibits...
Root-knot nematodes (RKN), from the Meloidogyne genus, have a worldwide distribution and cause severe economic damage to many life-sustaining crops. Because of their lack specificity danger environment, most chemical nematicides been banned use. Thus, there is great need for new safe compounds control RKN. Such research involves identifying beforehand nematode proteins essential invasion. Since G protein-coupled receptors GPCRs are target large number drugs, we focused our on identification...
Constitutive expression of Odorant-Binding Proteins (OBPs) in antennae and other body parts has been examined mainly to infer their involvement insect olfaction, while regulation response semiochemical stimuli remained poorly known. Previous studies were basically done using electrophysiology, which integrates the set OBPs present an antenna or sensillum, without revealing ones might be involved. In this study we used boll weevil as a model mined its by RNA-Seq simultaneous antennal qPCR...
Abstract Griffinia gardneriana Ravenna, liboniana Morren and nocturna Ravenna (Amarillydaceae) are bulbous plants found in tropical regions of Brazil. Our work aimed to determine the alkaloid profiles spp. evaluate their anxiolytic potential through vivo silico assays. The grown greenhouses were dried ground bulbs subjected liquid‐liquid partitions, resulting fractions that analyzed by gas chromatography coupled mass spectrometry (GC‐MS). Anxiolytic activity was evaluated zebrafish (Danio...
This paper reports the sequence analysis of Bm86 Campo Grande strain comparing it with and Bm95 antigens from preparations TickGardPLUS GavacTM, respectively. The PCR product was cloned into pMOSBlue sequenced. secondary structure prediction tool PSIPRED used to calculate alpha helices beta strand contents predicted polypeptide. hydrophobicity profile calculated using algorithms Hopp Woods method, in addition identification potential MHC class-I binding regions antigens. Pair-wise alignment...
Plants are continuously exposed to pathogen attack, but successful infection is rare because they protect themselves against pathogens using a wide range of response mechanisms. One them the hypersensitive (HR), which form cell death often associated with plant resistance prevent spreadsebpg@cnpq.br sebpg@cnpq.br potential from infected uninfected tissues. Cell activated by recognition pathogen-derived molecules (R) gene products, and massive accumulation reactive oxygen species (ROS),...