- Peanut Plant Research Studies
- Legume Nitrogen Fixing Symbiosis
- Plant tissue culture and regeneration
- Nematode management and characterization studies
- Nitrogen and Sulfur Effects on Brassica
- Coconut Research and Applications
- Plant Virus Research Studies
- Agricultural pest management studies
- Transgenic Plants and Applications
- Cassava research and cyanide
- Seed Germination and Physiology
- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
- Agricultural and Food Sciences
- Growth and nutrition in plants
- Plant pathogens and resistance mechanisms
- Plant Parasitism and Resistance
- Phytochemistry Medicinal Plant Applications
- Genetically Modified Organisms Research
- Soybean genetics and cultivation
- Plant Genetic and Mutation Studies
- Plant Pathogens and Fungal Diseases
- Plant Disease Management Techniques
- Agronomic Practices and Intercropping Systems
- Plant Pathogenic Bacteria Studies
Brazilian Agricultural Research Corporation
2015-2025
Biotech (Brazil)
2020-2024
Agricultural Research Corporation
2020
International Atomic Energy Agency
2014
Universidade Católica de Brasília
2014
Agropolis International
2002
Sociedade Brasileira de Biologia Celular
1997
Institut National de la Recherche Agronomique
1990-1994
Laboratoire de Génétique Cellulaire
1992
Centre National pour la Recherche Scientifique et Technique (CNRST)
1990
Abstract Background The WRKY transcription factor gene family has a very ancient origin and undergone extensive duplications in the plant kingdom. Several studies have pointed out their involvement range of biological processes, revealing that large number genes are transcriptionally regulated under conditions biotic and/or abiotic stress. To investigate existence co-regulatory networks plants, whole s expression study was carried rice ( Oryza sativa ). This analysis extended to Arabidopsis...
Abstract Background Rice ( Oryza sativa L.) germplasm represents an extraordinary source of genes that control traits agronomic importance such as drought tolerance. This diversity is the basis for development new cultivars better adapted to water restriction conditions, in particular upland rice, which grown under rainfall. The analyses subtractive cDNA libraries and differential protein expression tolerant susceptible genotypes can contribute understanding genetic use efficiency rice....
Abstract Background Cultivated peanut ( Arachis hypogaea ) is one of the most widely grown grain legumes in world, being valued for its high protein and unsaturated oil contents. Worldwide, major constraints to production are drought fungal diseases. Wild species, which exclusively South American origin, have genetic diversity been selected during evolution a range environments biotic stresses, constituting rich source allele diversity. stenosperma harbors resistances number pests, including...
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Abstract Raffinose family oligosaccharides (RFOs) are implicated in plant regulatory mechanisms of abiotic stresses tolerance and, despite their antinutritional proprieties grain legumes, little information is available about the enzymes involved RFO metabolism Fabaceae species. In present study, systematic survey legume proteins belonging to five key RFOs (galactinol synthase, raffinose stachyose alpha-galactosidase, and beta-fructofuranosidase) identified 28 coding-genes Arachis duranensis...
Wild peanut species (Arachis spp.) are a rich source of new alleles for improvement. Plant transcriptome analysis under specific experimental conditions helps the understanding cellular processes related, instance, to development, stress response, and crop yield. The validation these studies has been generally accomplished by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) which requires normalization mRNA levels among samples. This can be achieved comparing expression...
Wild peanut relatives (Arachis spp.) are genetically diverse and were adapted to a range of environments during the evolution course, constituting an important source allele diversity for resistance biotic abiotic stresses. The wild diploid A. stenosperma harbors high levels variety pathogens, including root-knot nematode (RKN) Meloidogyne arenaria, through onset Hypersensitive Response (HR). In order identify genes regulators triggering this defense response, comprehensive root...
Quantitative Polymerase Chain Reaction (qPCR) is currently the most sensitive technique used for absolute and relative quantification of a target gene transcript, requiring use appropriated reference genes data normalization. To accurately estimate expression tomato (Solanum lycopersicum L.) responsive to several virus species in reverse transcription qPCR analysis, identification reliable mandatory. In present study, ten were analyzed across set eight samples: two contrasting genotypes...
Cultivated peanut is an allotetraploid (genome type AABB) with a very narrow genetic base, therefore wild species are attractive source of new variability and traits. Because most diploid, the first step introgression usually involves hybridization polyploidization to produce synthetic (AABB) that sexually compatible peanut. This study investigates drought-related traits such as leaf morphology, transpiration profile, chlorophyll meter readings (SCMR), specific area (SLA) rate per for two...
Abstract Background Although banana ( Musa sp.) is an important edible crop, contributing towards poverty alleviation and food security, limited transcriptome datasets are available for use in accelerated molecular-based breeding this genus. 454 GS-FLX Titanium technology was employed to determine the sequence of gene transcripts genotypes acuminata ssp. burmannicoides Calcutta 4 M. subgroup Cavendish cv. Grande Naine, contrasting resistance fungal pathogen Mycosphaerella musicola , causal...
Background and AimsArachis batizocoi is a wild relative of cultivated peanut (A. hypogaea), an allotetraploid with AABB genome. Arachis was once considered the ancestral donor B genome, but cytogenetics DNA phylogenies have indicated new genome classification, 'K'. These observations seem inconsistent genetic studies breeding that shown A. can behave as Methods The behaviour, composition phylogenetic position were studied using controlled hybridizations, induced tetraploidy, whole-genome in...
Expansins are plant cell wall-loosening proteins involved in adaptive responses to environmental stimuli and various developmental processes. The first genome-wide analysis of the expansin superfamily Arachis genus identified 40 members A. duranensis 44 ipaënsis, wild progenitors cultivated peanut (A. hypogaea). These expansins were further characterized regarding their subfamily classification, distribution along genomes, duplication events, molecular structure, phylogeny. A RNA-seq...
Several species of Arachis have been cultivated for their edible seeds, historically and to the present day. The diploid that a history cultivation show relatively small signatures domestication. In contrast, tetraploid A. hypogaea evolved into highly domesticated forms became major world crop, peanut. It seems likely allotetraploidization (hybridity and/or tetraploidization) in some way enhanced attractiveness cultivation. Here we investigate this using six different hybridization...
Plant-parasitic nematodes cause extensive annual yield losses to worldwide agricultural production. Most cultivated plants have no known resistance against and the few bearing a gene can be overcome by certain species. Chemical methods that been deployed control largely banned from use due their poor specificity high toxicity. Hence, there is an urgent need for development of cleaner more specific methods. Recent advances in nematode genomics, including phytoparasitic species, provide...
Plant expansins are structural cell wall-loosening proteins implicated in several developmental processes and responses to environmental constraints pathogen infection. To date, there is limited information about the biological function of expansins-like B (EXLBs), one smallest less-studied subfamilies plant expansins. In present study, we conducted a functional analysis wild Arachis AdEXLB8 gene transgenic tobacco (Nicotiana tabacum) plants clarify its putative role mediating defense...
This study proposes using the CRISPR transcriptional activation strategy to modulate expression of genes encoding defense proteins and antimicrobial peptides (AMPs) in Phaseolus vulgaris. Three (PvD1, Pv-thionin, Pv-lectin) were selected targeted by CRISPR-dCas9-TV-mediated complex P. vulgaris L. hairy root. RT-qPCR investigated their efficiency. The eGFP-positive transgenic roots exhibit enhanced compared that control roots. A moderate increase 1.37-fold PvD1 gene was observed roots, while...