David J. Bertioli

ORCID: 0000-0003-0294-7284
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About
Contact & Profiles
Research Areas
  • Peanut Plant Research Studies
  • Coconut Research and Applications
  • Agricultural pest management studies
  • Nitrogen and Sulfur Effects on Brassica
  • Plant Virus Research Studies
  • Legume Nitrogen Fixing Symbiosis
  • Nematode management and characterization studies
  • Chromosomal and Genetic Variations
  • Insect Resistance and Genetics
  • Entomopathogenic Microorganisms in Pest Control
  • Banana Cultivation and Research
  • Plant Disease Management Techniques
  • Agronomic Practices and Intercropping Systems
  • Plant and Fungal Interactions Research
  • Cassava research and cyanide
  • Plant Disease Resistance and Genetics
  • Molecular Biology Techniques and Applications
  • Plant tissue culture and regeneration
  • Plant-Microbe Interactions and Immunity
  • RNA and protein synthesis mechanisms
  • Soybean genetics and cultivation
  • Genomics and Phylogenetic Studies
  • Transgenic Plants and Applications
  • Genetic and Environmental Crop Studies
  • Nuts composition and effects

University of Georgia
2016-2025

Applied Genetic Technologies (United States)
2015-2024

Brazilian Agricultural Research Corporation
1996-2020

IAC (United States)
2020

Governo do Estado de São Paulo
2020

Universidade de Brasília
2008-2017

Universidade Católica de Brasília
2004-2014

International Atomic Energy Agency
2014

Texas Tech University
2008

Instituto Multidisciplinario de Biología Vegetal
2007

David Bertioli and colleagues report the genomes of Arachis duranensis ipaensis, diploid ancestors cultivated peanut, hypogaea. Their analyses are a first step in understanding evolution peanut's tetraploid genome. Cultivated peanut (Arachis hypogaea) is an allotetraploid with closely related subgenomes total size ∼2.7 Gb. This makes assembly chromosomal pseudomolecules very challenging. As foundation to genome we sequences its ipaensis). We show that these similar A B use them identify...

10.1038/ng.3517 article EN cc-by-nc-sa Nature Genetics 2016-02-22

Like many other crops, the cultivated peanut (Arachis hypogaea L.) is of hybrid origin and has a polyploid genome that contains essentially complete sets chromosomes from two ancestral species. Here we report sequence show after its origin, evolved through mobile-element activity, deletions by flow genetic information between corresponding (that is, homeologous recombination). Uniformity patterns recombination at ends favors single for wild counterpart A. monticola. However, much genome,...

10.1038/s41588-019-0405-z article EN cc-by Nature Genetics 2019-05-01

Simple sequence repeats (SSR), also known as microsatellites, have been extensively used molecular markers due to their abundance and high degree of polymorphism. We developed a simple use web software, called WebSat, for microsatellite marker prediction development. WebSat is accessible through the Internet, requiring no program installation. Although solution, it makes Ajax techniques, providing rich, responsive user interface. allows submission sequences, visualization microsatellites...

10.6026/97320630003282 article EN cc-by Bioinformation 2009-01-12

Molecular markers and genetic linkage maps are pre-requisites for molecular breeding in any crop species. In case of peanut or groundnut (Arachis hypogaea L.), an amphidiploid (4X) species, not a single map is, however, available based on mapping population derived from cultivated genotypes. order to develop tetraploid groundnut, total 1,145 microsatellite simple sequence repeat (SSR) public domain as well unpublished several sources were screened two genotypes, TAG 24 ICGV 86031 that...

10.1007/s00122-008-0933-x article EN cc-by-nc Theoretical and Applied Genetics 2008-12-01

Cultivated groundnut or peanut (Arachis hypogaea L.), an allotetraploid (2n = 4x 40), is a self pollinated and widely grown crop in the semi-arid regions of world. Improvement drought tolerance important area research for breeding programmes. Therefore, identification candidate QTLs tolerance, comprehensive refined genetic map containing 191 SSR loci based on single mapping population (TAG 24 x ICGV 86031), segregating surrogate traits was developed. Genotyping data phenotyping collected...

10.1007/s00122-010-1517-0 article EN cc-by-nc Theoretical and Applied Genetics 2010-12-29

Arachis hypogaea is a natural, well‐established allotetraploid (AABB) with 2 n = 40. However, researchers disagree on the diploid genome donor species and whether peanut originated by single or multiple events of polyploidization. Here we provide evidence genetic origin involved wild relatives using double GISH (genomic in situ hybridization). Seven (2 20), harboring either A B genome, were tested. Of all genomic DNA probe combinations assayed, A. duranensis (A genome) ipaensis (B appeared...

10.3732/ajb.94.12.1963 article EN American Journal of Botany 2007-12-01

The genus Arachis contains 80 described species. Section is of particular interest because it includes cultivated peanut, an allotetraploid, and closely related wild species, most which are diploids. This study aimed to analyse the genetic relationships multiple accessions section species using two complementary methods. Microsatellites allowed analysis inter- intraspecific variability. Intron sequences from single-copy genes phylogenetic including separation allotetraploid genome...

10.1093/aob/mcs237 article EN Annals of Botany 2012-11-06

Abstract Single nucleotide polymorphisms (SNPs) are the most abundant DNA sequence variation in genomes which can be used to associate genotypic phenotype. Therefore, availability of a high-density SNP array with uniform genome coverage advance genetic studies and breeding applications. Here we report development ‘Axiom _Arachis ’ 58 K SNPs its utility groundnut diversity study. In this context, from total 163,782 derived resequencing RNA-sequencing 41 accessions wild diploid ancestors,...

10.1038/srep40577 article EN cc-by Scientific Reports 2017-01-16

The legume Arachis hypogaea , commonly known as peanut or groundnut, is a very important food crop throughout the tropics and sub-tropics. genus endemic to South America being mostly associated with savannah-like Cerrado. All species in are unusual among legumes that they produce their fruit below ground. This profoundly influences biology natural distributions. occur diverse habitats including grasslands, open patches of forest even temporarily flooded areas. Based on number criteria,...

10.1017/s1479262110000444 article EN Plant Genetic Resources 2011-01-14

Most agriculturally important legumes fall within two sub-clades of the Papilionoid legumes: Phaseoloids and Galegoids, which diverged about 50 Mya. The are mostly tropical include crops such as common bean soybean. Galegoids temperate clover, fava model Lotus Medicago (both with substantially sequenced genomes). In contrast, peanut (Arachis hypogaea) falls in Dalbergioid clade is more basal its divergence Papilionoids. aim this work was to integrate genetic map Arachis improve our...

10.1186/1471-2164-10-45 article EN cc-by BMC Genomics 2009-01-01

Worldwide, diseases are important reducers of peanut (Arachis hypogaea) yield. Sources resistance against many available in cultivated genotypes, although often not farmer preferred varieties. Wild species generally harbor greater levels and even apparent immunity, the linkage agronomically un-adapted wild alleles with disease genes is inevitable. Marker-assisted selection has potential to facilitate combination both loci adapted alleles. However, there an almost complete lack knowledge...

10.1186/1471-2229-9-112 article EN cc-by BMC Plant Biology 2009-01-01

Peanut (Arachis hypogaea; 2n = 4x 40) is a nutritious food and good source of vitamins, minerals, healthy fats. Expansion genetic genomic resources for enhancement cultivated peanut has gained momentum from the sequenced genomes diploid ancestors peanut. To facilitate high-throughput genotyping Arachis species, 20 genotypes were re-sequenced genome-wide single nucleotide polymorphisms (SNPs) selected to develop large-scale SNP array. For flexibility in applications, SNPs polymorphic between...

10.1016/j.molp.2016.11.015 article EN cc-by Molecular Plant 2016-12-18

The complex, tetraploid genome structure of peanut (Arachis hypogaea) has obstructed advances in genetics and genomics the species. aim this study is to understand Arachis by developing a high-density integrated consensus map. Three recombinant inbred line populations derived from crosses between A diploid species, duranensis stenosperma; B ipaënsis magna; AB tetraploids, A. hypogaea an artificial amphidiploid (A. × duranensis)4×, were used construct genetic linkage maps: 10 groups (LGs) 544...

10.1093/dnares/dss042 article EN cc-by-nc DNA Research 2013-01-12

Abstract Background Polyploidy can result in genetic bottlenecks, especially for species of monophyletic origin. Cultivated peanut is an allotetraploid harbouring limited diversity, likely resulting from the combined effects its single origin and domestication. Peanut wild relatives represent important source novel alleles that could be used to broaden basis cultigen. Using advanced backcross population developed with a synthetic amphidiploid as donor alleles, under two water regimes, we...

10.1186/1471-2229-12-26 article EN cc-by BMC Plant Biology 2012-02-17

Summary Whole‐genome resequencing ( WGRS ) of mapping populations has facilitated development high‐density genetic maps essential for fine and candidate gene discovery traits interest in crop species. Leaf spots, including early leaf spot ELS late LLS ), Tomato spotted wilt virus TSWV are devastating diseases peanut causing significant yield loss. We generated data on a recombinant inbred line population, developed SNP ‐based map, conducted mapping, marker validation , . The first...

10.1111/pbi.12930 article EN cc-by Plant Biotechnology Journal 2018-04-11

Abstract Background Due to its origin, peanut has a very narrow genetic background. Wild relatives can be source of variability for cultivated peanut. In this study, the transcriptome wild species Arachis stenosperma accession V10309 was analyzed. Results ESTs were produced from four cDNA libraries RNAs extracted leaves and roots A. . Randomly selected clones sequenced generate 8,785 ESTs, which 6,264 (71.3%) had high quality, with 3,500 clusters: 963 contigs 2537 singlets. Only 55.9%...

10.1186/1471-2229-7-7 article EN cc-by BMC Plant Biology 2007-02-15

Arachis hypogaea (peanut) is an important crop worldwide, being mostly used for edible oil production, direct consumption and animal feed. Cultivated peanut allotetraploid species with two different genome components, A B. Genetic linkage maps can greatly assist molecular breeding genomic studies. However, the development of A. difficult because it has very low levels polymorphism. This be overcome by utilization wild Arachis, which present A- B-genomes in diploid state, show high genetic...

10.1186/1471-2229-9-40 article EN cc-by BMC Plant Biology 2009-01-01

Abstract Background Peanut ( Arachis hypogaea L.) is widely used as a food and cash crop around the world. It considered to be an allotetraploid (2n = 4x 40) originated from single hybridization event between two wild diploids. The most probable hypothesis gave A. duranensis donor of A genome ipaënsis B genome. low level molecular polymorphism found in cultivated germplasm up date few genetic linkage maps have been published. utilization breeding programs has received little attention due...

10.1186/1471-2229-9-103 article EN cc-by BMC Plant Biology 2009-08-03

Only a few genetic maps based on recombinant inbred line (RIL) and backcross (BC) populations have been developed for tetraploid groundnut. The marker density, however, is not very satisfactory especially in the context of large genome size (2800 Mb/1C) 20 linkage groups (LGs). Therefore, using segregation data 10 RILs one BC population from international groundnut community, with help common markers across different populations, reference consensus map has developed. This comprised 897 loci...

10.1371/journal.pone.0041213 article EN cc-by PLoS ONE 2012-07-18

Abstract Background Cultivated peanut ( Arachis hypogaea L.) is an important crop worldwide, valued for its edible oil and digestible protein. It has a very narrow genetic base that may well derive from relatively recent single polyploidization event. Accordingly molecular markers have low levels of polymorphism the number polymorphic available cultivated still limiting. Results Here, we report large set BAC-end sequences (BES), use them developing SSR (BES-SSR) markers, apply in linkage...

10.1186/1471-2229-12-10 article EN cc-by BMC Plant Biology 2012-01-19

Abstract Background Cultivated peanut ( Arachis hypogaea ) is one of the most widely grown grain legumes in world, being valued for its high protein and unsaturated oil contents. Worldwide, major constraints to production are drought fungal diseases. Wild species, which exclusively South American origin, have genetic diversity been selected during evolution a range environments biotic stresses, constituting rich source allele diversity. stenosperma harbors resistances number pests, including...

10.1186/1471-2164-13-387 article EN cc-by BMC Genomics 2012-08-13
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