A5066164830- Peanut Plant Research Studies
- Agricultural pest management studies
- Coconut Research and Applications
- Plant Taxonomy and Phylogenetics
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Nitrogen and Sulfur Effects on Brassica
- Transgenic Plants and Applications
- Plant and fungal interactions
- Plant Genetic and Mutation Studies
- Chromosomal and Genetic Variations
- Botanical Research and Chemistry
- Legume Nitrogen Fixing Symbiosis
- Plant Disease Resistance and Genetics
- Seed Germination and Physiology
- Food Allergy and Anaphylaxis Research
- Seedling growth and survival studies
- Soybean genetics and cultivation
- Genetic Mapping and Diversity in Plants and Animals
- Agronomic Practices and Intercropping Systems
- Bioenergy crop production and management
- Botany and Plant Ecology Studies
- Genetically Modified Organisms Research
- Plant Reproductive Biology
- Turfgrass Adaptation and Management
University of Georgia
2016-2025
Georgia College & State University
2024
Applied Genetic Technologies (United States)
2020-2021
Child Trends
2020
University of Nottingham
2020
Agricultural Research Service
1994-2016
University of Georgia Research Foundation
2012
University of Missouri
2008
Texas A&M University
2004-2008
Texas Tech University
2008
David Bertioli and colleagues report the genomes of Arachis duranensis ipaensis, diploid ancestors cultivated peanut, hypogaea. Their analyses are a first step in understanding evolution peanut's tetraploid genome. Cultivated peanut (Arachis hypogaea) is an allotetraploid with closely related subgenomes total size ∼2.7 Gb. This makes assembly chromosomal pseudomolecules very challenging. As foundation to genome we sequences its ipaensis). We show that these similar A B use them identify...
Like many other crops, the cultivated peanut (Arachis hypogaea L.) is of hybrid origin and has a polyploid genome that contains essentially complete sets chromosomes from two ancestral species. Here we report sequence show after its origin, evolved through mobile-element activity, deletions by flow genetic information between corresponding (that is, homeologous recombination). Uniformity patterns recombination at ends favors single for wild counterpart A. monticola. However, much genome,...
Pearl millet [Cenchrus americanus (L.) Morrone] is a staple food for more than 90 million farmers in arid and semi-arid regions of sub-Saharan Africa, India South Asia. We report the ∼1.79 Gb draft whole genome sequence reference genotype Tift 23D2B1-P1-P5, which contains an estimated 38,579 genes. highlight substantial enrichment wax biosynthesis genes, may contribute to heat drought tolerance this crop. resequenced analyzed 994 pearl lines, enabling insights into population structure,...
Kompetitive allele‐specific polymerase chain reaction (KASP) assays have emerged as cost‐effective marker especially for molecular breeding applications. Therefore, a set of 96 informative single nucleotide polymorphisms (SNPs) was used to develop KASP in groundnut or peanut ( Arachis spp.). Developed were designated assay markers (GKAMs) and screened on 94 genotypes (validation set) that included parental lines 27 mapping populations, seven synthetic autotetraploid amphidiploid lines, 19...
The advent of the genome sequences Arachis duranensis and ipaensis has ushered in a new era for peanut genomics. With goal producing gene atlas cultivated (Arachis hypogaea), 22 different tissue types ontogenies that represent full development were sequenced, including complete reproductive series from flower to peg elongation tip immersion soil fully mature seed. Using genome-guided assembly pipeline, homeolog-specific transcriptome hypogaea was assembled its accuracy validated. used...
Summary Plant genetic transformation is a crucial step for applying biotechnology such as genome editing to basic and applied plant science research. Its success primarily relies on the efficiency of gene delivery into cells ability regenerate transgenic plants. In this study, we have examined effect several developmental regulators (DRs), including PLETHORA (PLT5) , WOUND INDUCED DEDIFFERENTIATION 1 (WIND1) ENHANCED SHOOT REGENERATION (ESR1) WUSHEL (WUS) fusion WUS BABY‐BOOM ( WUS‐P2A‐BBM...
ABSTRACT Tobacco plants ( Nicotiana tabacum L.) were transformed with a mannitol‐1‐phosphate dehydrogenase gene resulting in mannitol accumulation. Experiments conducted to determine whether provides salt and/or drought stress protection through osmotic adjustment. Non‐stressed transgenic 20–25% smaller than non‐stressed, non‐transformed (wild‐type) both salinity and experiments. However, reduced dry weight wild‐type by 44%, but did not reduce the of plants. Transgenic adjusted osmotically...
Apomixis is a naturally occurring mode of reproduction that results in embryo formation without the involvement meiosis or fertilization egg. Seed-derived progeny an apomictic plant are genetically identical to maternal parent. We studying form apomixis called apospory occurs genus Pennisetum , taxon grass family. A cultivated member this genus, pearl millet ( glaucum ), reproduces sexually. wild relative millet, squamulatum obligate aposporous species, cross-compatible with when used as...
The dynamic challenges of peanut (Arachis hypogaea L.) farming demand a quick response from breeders to develop new cultivars, process that can be aided by the application molecular markers. With goal pyramid nematode resistance and trait for high oleic:linoleic acid (high O:L) ratio in seeds, nematode-resistant cultivar Tifguard was used as recurrent female parent O:L cultivars Georgia-02C Florida-07 were donor parents trait. 'Tifguard High O/L' generated through three rounds accelerated...
Apomixis is a naturally occurring mode of asexual reproduction in flowering plants that results seed formation without the involvement meiosis or fertilization egg. Seeds formed on an apomictic plant contain offspring genetically identical to maternal plant. has significant potential for preserving hybrid vigor from one generation next highly productive crop genotypes. Apomictic Pennisetum/Cenchrus species, members Poaceae (grass) family, reproduce by apospory. Apospory characterized...
Cell suspension cultures of Triticum monococcum, Panicum maximum, Saccharum officinarum, Pennisetum americanum, and a double cross trispecific hybrid between P. purpureum, squamulatum were tested for resistance to kanamycin, hygromycin, methotrexate use in transformation studies. All showed high natural levels excess 800 milligrams per liter, variable hygromycin. Methotrexate was potent growth inhibitor at low concentrations with all species. Kanamycin hygromycin inhibitory only if added...
Abstract Single nucleotide polymorphisms (SNPs) are the most abundant DNA sequence variation in genomes which can be used to associate genotypic phenotype. Therefore, availability of a high-density SNP array with uniform genome coverage advance genetic studies and breeding applications. Here we report development ‘Axiom _Arachis ’ 58 K SNPs its utility groundnut diversity study. In this context, from total 163,782 derived resequencing RNA-sequencing 41 accessions wild diploid ancestors,...
Peanut (Arachis hypogaea; 2n = 4x 40) is a nutritious food and good source of vitamins, minerals, healthy fats. Expansion genetic genomic resources for enhancement cultivated peanut has gained momentum from the sequenced genomes diploid ancestors peanut. To facilitate high-throughput genotyping Arachis species, 20 genotypes were re-sequenced genome-wide single nucleotide polymorphisms (SNPs) selected to develop large-scale SNP array. For flexibility in applications, SNPs polymorphic between...
Late leaf spot (LLS; Cercosporidium personatum) is a major fungal disease of cultivated peanut (Arachis hypogaea). A recombinant inbred line population segregating for quantitative field resistance was used to identify trait loci (QTL) using QTL-seq. High rates false positive SNP calls established methods in this allotetraploid crop obscured significant QTLs. To resolve problem, robust parental SNPs were first identified polyploid-specific identification pipelines, leading discovery QTLs LLS...
Summary Whole‐genome resequencing ( WGRS ) of mapping populations has facilitated development high‐density genetic maps essential for fine and candidate gene discovery traits interest in crop species. Leaf spots, including early leaf spot ELS late LLS ), Tomato spotted wilt virus TSWV are devastating diseases peanut causing significant yield loss. We generated data on a recombinant inbred line population, developed SNP ‐based map, conducted mapping, marker validation , . The first...
Multiparental genetic mapping populations such as nested-association (NAM) have great potential for investigating quantitative traits and associated genomic regions leading to rapid discovery of candidate genes markers. To demonstrate the utility power this approach, two NAM populations, NAM_Tifrunner NAM_Florida-07, were used dissecting control 100-pod weight (PW) 100-seed (SW) in peanut. Two high-density SNP-based maps constructed with 3341 loci 2668 respectively. The trait locus (QTL)...
SUMMARY Cowpea ( Vigna unguiculata (L.) Walp.) is one of the most important legume crops planted worldwide, but despite decades effort, cowpea transformation still challenging due to inefficient Agrobacterium ‐mediated transfer DNA delivery, transgenic selection and in vitro shoot regeneration. Here, we report a highly efficient system using embryonic axis explants isolated from imbibed mature seeds. We found that removal apical meristem stimulated direct multiple organogenesis cotyledonary...