Streptomycetes are a complex group of actinomycetes that produce diverse bioactive metabolites commercial significance. Systematics can provide useful framework for identifying species may novel metabolites. However, previously proposed approaches to the systematics Streptomyces have suffered from either poor interlaboratory comparability or insufficient resolution. In particular, griseus 16S rRNA gene clade is most challenging and least defined within genus in terms phylogeny. Here we...

RNA-seq and microarray are the two popular methods employed for genome-wide transcriptome profiling. Current comparison studies have shown that quantified by these correlated well. However, none of them addressed if they complement each other, considering strengths limitations inherent with them. The pivotal requirement to address this question is knowledge a well known data set. In regard, HrpX regulome from pathogenic bacteria serves as an ideal choice target genes transcription factor...

SUMMARY Cowpea ( Vigna unguiculata (L.) Walp.) is one of the most important legume crops planted worldwide, but despite decades effort, cowpea transformation still challenging due to inefficient Agrobacterium ‐mediated transfer DNA delivery, transgenic selection and in vitro shoot regeneration. Here, we report a highly efficient system using embryonic axis explants isolated from imbibed mature seeds. We found that removal apical meristem stimulated direct multiple organogenesis cotyledonary...

Xanthomonas axonopodis pv. citri is the causal agent of citrus canker, which one most serious diseases citrus. To understand virulence mechanisms X. citri, we designed and conducted genome-wide microarray analyses to characterize HrpG HrpX regulons, are critical for pathogenicity citri. Our revealed that 232 181 genes belonged respectively. In total, 123 were overlapped in two regulons at any three selected timepoints representing growth stages XVM2 medium. results showed regulated all 24...

To cause infection, Salmonella must survive and replicate in host niches that present dramatically different environmental conditions. This requires a flexible metabolism physiology, responsive to conditions of the local milieu. The sequence specific RNA binding protein CsrA serves as global regulator governs gene expression required for pathogenicity, metabolism, biofilm formation, motility response nutritional Its activity is determined by two noncoding small RNAs (sRNA), CsrB CsrC, which...

Camel milk has unique compositional, functional and therapeutic properties compared to cow's also contains many protective proteins with anti-cancer, anti-diabetic anti-bacterial properties. In this experiment, fresh camel was heat-treated at different temperatures times, the changes in Millard reaction products were analyzed. Meanwhile, headspace-gas chromatography-ion migration spectrometry (HS-GC-IMS), electronic nose tongue used analyze of volatile components after heat treatments. The...

N6-Methyladenine (6mA) of DNA has emerged as a novel epigenetic mark in eukaryotes, and several 6mA effector proteins have been identified. However, efforts to selectively inhibit the biological functions these with small molecules are unsuccessful date. Here we report first potent selective molecule inhibitor (13h) AlkB homologue 1 (ALKBH1), only validated demethylase. 13h showed an IC50 0.026 ± 0.013 μM 1.39 0.13 fluorescence polarization (FP) enzyme activity assay, respectively, KD 0.112...

ABSTRACT Xanthomonas citri subsp. is the causal agent of citrus canker, which has a significant impact on production. In this study, we characterized galU gene X. . Two mutants (F6 and D12) were identified in an EZ-Tn5 <R6Kγori/KAN-2> Tnp transposon library. Rescue cloning, sequence analysis, Southern blot analysis indicated that both these had single copy inserted chromosome. Further study showed was required for biosynthesis extracellular polysaccharides (EPS; xanthan gum) capsular...

Diffusible signal factor (DSF) family signal-mediated quorum sensing (QS) has been identified in many gram-negative bacteria. This QS pathway of Xanthomonas spp. consists three major components: RpfF, RpfC, and RpfG. The rpfF gene encodes a putative enoyl-CoA hydratase that catalyzes the synthesis molecule. RpfC RpfG serve as two-component system for perception transduction extracellular DSF signals. In order to further characterize regulatory network citri subsp. citri, we investigated...

Background: Ginsenoside compound K (GC-K), generated from ginseng saponins bioconverted by gut microbiota, has potential anti-colorectal cancer (CRC) effects. Meanwhile, GC-K may interact with playing important roles in the occurrence and development of CRC. However, effects microbiota on preventive therapeutic CRC remain to be elucidated. Methods: The anti-CRC were evaluated an azoxymethane/dextran sulfate sodium (AOM/DSS)-induced colitis-associated Balb/c mice model under dosage 30 60...

Summary Background Several studies have indicated that the triglyceride-glucose index (TyG) is associated with hypertension; however, evidence on association of change in TyG blood pressure and hypertension limited. Aims To assess hypertension. Design A cohort study. Methods We included 17 977 individuals a mean age 60.5 years from Dongfeng–Tongji cohort. The was calculated as ln [fasting triglyceride (mg/dl)×fasting glucose (mg/dl)/2]. Hypertension defined ≥140/90 mmHg, self-reported...

Background: Engineering apomixis in sexually reproducing plants has been long desired because of the potential to fix hybrid vigor. Validating functionality genes originated from apomictic species that contribute upon transfer is an important step. The PsASGR-BABYBOOM-like (PsASGR-BBML) gene Pennisetum squamulatum confers parthenogenesis this apomict, and its was demonstrated several monocots but not any dicots. Methods: We introduced PsASGR-BBML regulated by egg cell-specific promoters,...

Panax notoginseng saponins (PNSs) are the major health-beneficial components of P. with very low oral bioavailability, which could be biotransformed by gut microbiota in vitro. However, vivo biotransformation PNS mediated is not well known. This study aimed to characterize metabolic profiles microbiota. The and yielded metabolites rat feces were identified relatively quantified ultra-performance liquid chromatography tandem/quadrupole time-of-flight mass spectrometry. Seventy-three had been...

Insulin resistance was reported to increase the risk of ischemic stroke, which can be assessed by triglyceride glucose (TyG) index. However, it remains unclear whether TyG index influences platelet reactivity during treatment patients.Ischemic stroke patients receiving dual antiplatelet therapy (DAPT) within 48 h onset were consecutively included. The calculated as ln (fasting [mg/dL] × fasting [mg/dL]/2). top quartile defined insulin resistance. thromboelastography. inhibition rate induced...

The presence of Arbuscular Mycorrhizal Fungi (AMF) in vascular land plant roots is one the most ancient symbioses supporting nitrogen and phosphorus exchange for photosynthetically derived carbon. Here we provide a multi-scale modeling approach to predict AMF colonization worldwide crop from Recombinant Inbred Line (RIL) population Sorghum bicolor S. propinquum. high-throughput phenotyping methods fungal structures here rely on Mask Region-based Convolutional Neural Network (Mask R-CNN)...

Right-to-left shunt (RLS) induced by a patent foramen ovale (PFO) is associated with an increased risk of cryptogenic stroke (CS). However, little known about the relationship between amount RLS and pattern. In this study, we aimed to evaluate distinct clinical features PFO-related CS in different degrees resulting from PFO.This cohort study 222 patients admitted Tongji Hospital 1st May 2014 31st April 2017. All underwent contrast transcranial Doppler examination. And then, 121 (54.5%) were...

Due to the heterogeneity of amyloid β-42 (Aβ42) species, potential correlation between plasma oligomeric Aβ42 (oAβ42) and cognitive impairments in cerebral small vessel disease (CSVD) remains unclear. Herein, a sandwich ELISA for specific detection oligomers total (tAβ42) was developed based on sequence- conformation-specific antibody pairs evaluation samples from Chinese CSVD community cohort. After age gender matching, 3-Tesla magnetic resonance imaging multidimensional assessment were...

Abstract Utilizing a microfluidic chip with serpentine channels, we inoculated the an agar plug Neurospora crassa mycelium and successfully captured individual hyphae in channels. For first time, report presence of autonomous clock hyphae. Fluorescence mCherry reporter gene driven by clock-controlled gene-2 promoter ( ccg-2p ) was measured simultaneously along every half hour for at least 6 days. We entrained single to light over wide range day lengths, including 6,12, 24, 36 h Hyphae...

The higher than expected PFO rate in CS patients has raised concerns that paradoxical embolism maybe the pathophysiologic mechanism for strokes. However, only a small proportion of pathogenic PFOs cause CS. Therefore, accurate recognition with among all could guide clinical decision making selecting most appropriate treatment. aim this study was to devise new algorithm stratify cryptogenic stroke (CS) into patent foramen ovale (p-PFO)- and non-p-PFO-related patients.A total 1201 acute...

Abstract Background Ginsenoside CK (GCK) serves as the potential anti-colorectal cancer (CRC) protopanaxadiol (PPD)-type saponin, which could be mainly bio-converted to yield PPD by gut microbiota. Meanwhile, anti-CRC effects of GCK altered microbiota due their different diversity in CRC patients. We aimed investigate bioconversion variation mediated from patients comparing with healthy subjects. Methods Gut profiled 16S rRNA gene sequencing were collected volunteers and was incubated vitro....