A5024372488- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Animal Nutrition and Physiology
- Burkholderia infections and melioidosis
- Bacteriophages and microbial interactions
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Vibrio bacteria research studies
- Livestock and Poultry Management
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- Bacterial Genetics and Biotechnology
- Genetic and phenotypic traits in livestock
- Gut microbiota and health
- Antibiotic Resistance in Bacteria
- Immune Response and Inflammation
- Microbial infections and disease research
- Insect symbiosis and bacterial influences
- Genomics and Phylogenetic Studies
- Veterinary medicine and infectious diseases
- T-cell and B-cell Immunology
- Brucella: diagnosis, epidemiology, treatment
- Animal Virus Infections Studies
- Aquaculture disease management and microbiota
Roslin Institute
2016-2025
University of Edinburgh
2016-2025
British Beet Research Organisation
2023
Norwich Research Park
2021
Edinburgh College
2020
The Pirbright Institute
2006-2018
Biotechnology and Biological Sciences Research Council
2008-2014
Broomfield Hospital
2011
Institute for Marine Biosciences
2009
At Bristol
2008
We have determined the complete genome sequences of a host-promiscuous Salmonella enterica serovar Enteritidis PT4 isolate P125109 and chicken-restricted Gallinarum 287/91. Genome comparisons between these other isolates indicate that S . 287/91 is recently evolved descendent S. Enteritidis. Significantly, has undergone extensive degradation through deletion pseudogene formation. Comparison pseudogenes in with those identified previously host-adapted bacteria reveals loss many common...
Burkholderia pseudomallei is the causative agent of melioidosis, a serious infectious disease humans and animals that endemic in subtropical areas. B. facultative intracellular pathogen may invade survive within eukaryotic cells for prolonged periods. After internalization, bacteria escape from endocytic vacuoles into cytoplasm infected form membrane protrusions by inducing actin polymerization at one pole. It believed survival phagocytic cell-to-cell spread via required full virulence. We...
The severity of infections caused by Salmonella enterica serovar Typhimurium varies depending on the host species. Numerous virulence genes have been identified in S. Typhimurium, largely from studies mice, but their roles other species remain unclear. In most comprehensive survey its kind, through use signature-tagged mutagenesis we mutants that were unable to colonize calf intestines, chick intestines and both type three secretion systems encoded pathogenicity islands (SPIs) 1 2 required...
Enterohaemorrhagic Escherichia coli (EHEC) cause acute gastroenteritis in humans that may be complicated by life-threatening systemic sequelae. The predominant EHEC serotype affecting the UK and North America is O157 : H7 infections are frequently associated with contact ruminant faeces. Strategies to reduce carriage of ruminants expected lower incidence human infections; however, molecular mechanisms underlying persistence poorly understood. This paper reports first comprehensive survey for...
Chickens, pigs, and cattle are key reservoirs of Salmonella enterica, a foodborne pathogen worldwide importance. Though decade has elapsed since publication the first genome, thousands genes remain hypothetical or unknown function, basis colonization reservoir hosts is ill-defined. Moreover, previous surveys role in vivo have focused on systemic virulence murine typhoid models, genetic intestinal persistence thus zoonotic transmission received little study. We therefore screened pools random...
Melioidosis is a severe infectious disease of animals and humans caused by the Gram-negative intracellular pathogen Burkholderia pseudomallei . An Inv/Mxi-Spa-like type III protein secretion apparatus, encoded B. bsa locus, facilitates bacterial invasion epithelial cells, escape from endocytic vesicles survival. This study investigated role Bsa system in pathogenesis melioidosis murine models. bipD mutants, lacking component translocation were found to be significantly attenuated following...
Summary Burkholderia pseudomallei is a Gram‐negative facultative intracellular pathogen that enters and escapes from eukaryotic cells using the power of actin polymerization. We have identified bacterial protein (BimA) required for ability B. to induce formation tails. BimA contains proline‐rich motifs WH2‐like domains shares limited homology at C‐terminus with Yersinia autosecreted adhesin YadA. located pole cell which polymerization occurs mutation bimA abolished actin‐based motility in...
Amino acids are key carbon and energy sources for the asaccharolytic food-borne human pathogen Campylobacter jejuni. During microaerobic growth in amino acid rich complex media, aspartate, glutamate, proline serine only significantly utilized by strain NCTC 11168. The catabolism of aspartate glutamate was investigated. An aspartase (aspA) mutant (unable to utilize any except serine) a Cj0762c (aspB) lacking aspartate:glutamate aminotransferase glutamate), were severely impaired an aspA sdaA...
The cucumber mosaic virus (CMV) 2b protein not only inhibits anti-viral RNA silencing but also quenches transcriptional responses of plant genes to jasmonic acid, a key signalling molecule in defence against insects. This suggested that it might affect interactions between infected plants and aphids, insects transmit CMV. We found infection tobacco with gene deletion mutant (CMVΔ2b) induced strong resistance aphids (Myzus persicae) while CMV fostered aphid survival. Using electrical...
Two lineages of Salmonella enterica serovar Typhimurium (S. Typhimurium) multi-locus sequence type ST313 have been linked with the emergence invasive disease across sub-Saharan Africa. The expansion these has a temporal association HIV pandemic and antibiotic usage. We analysed whole genome 129 isolates representative two found evidence lineage-specific degradation, some similarities to that observed in S. Typhi. Individual exhibit distinct metabolic signature modified enteropathogenesis...
ABSTRACT We report the characterization of BopE, a type III secreted protein that is encoded adjacent to Burkholderia pseudomallei bsa locus and homologous Salmonella enterica SopE/SopE2. Inactivation bopE impaired bacterial entry into HeLa cells, indicating BopE facilitates invasion. Consistent with this notion, expressed in eukaryotic cells induced rearrangements subcortical actin cytoskeleton, purified exhibited guanine nucleotide exchange factor activity for Cdc42 Rac1 vitro.
ABSTRACT Type III secretion systems (T3SS) are conserved in many pathogenic gram-negative bacteria. Small molecules that specifically target T3SS Yersinia and Chlamydia spp. have recently been identified. Here we show two such compounds inhibit Salmonella T3SS-1, preventing of T3SS-1 effectors, invasion cultured epithelial cells, enteritis vivo.
ABSTRACT Previously, we identified five genes (Cj1321 to Cj1326, of which Cj1325 and Cj1326 are a single gene) in the O-linked flagellin glycosylation island that highly prevalent Campylobacter jejuni isolates from chickens. We report mutagenesis, functional, structural data confirm this locus, Cj1324 particular, has significant contributory role colonization chickens by C. . A motile ΔCj1324 mutant with intact flagella was considerably less hydrophobic able autoagglutinate form biofilms...
The mechanisms by which RND pumps contribute to pathogenicity are currently not understood. Using the AcrAB-TolC system as a paradigm multidrug-resistant efflux pump and Salmonella enterica serovar Typhimurium model pathogen, we have demonstrated that AcrA, AcrB, TolC each required for efficient adhesion invasion of epithelial cells macrophages in vitro. In addition, AcrB necessary colonize poultry. Mutants lacking acrA, acrB, or tolC showed differential expression major operons proteins...
ABSTRACT Burkholderia pseudomallei is the causative pathogen of melioidosis, which a major predisposing factor diabetes mellitus. Polymorphonuclear neutrophils (PMNs) kill microbes extracellularly by release neutrophil extracellular traps (NETs). PMNs play key role in control but involvement NETs killing B. remains obscure. Here, we showed that bactericidal were released from human response to dose- and time-dependent manner. -induced NET formation required NADPH oxidase activation not...
ABSTRACT Avian pathogenic Escherichia coli (APEC) causes respiratory and systemic disease in poultry. Sequencing of a multilocus sequence type 95 (ST95) serogroup O1 strain previously indicated that APEC resembles E. causing extraintestinal human diseases. We sequenced the genomes two strains another dominant lineage (ST23 O78 χ7122 IMT2125) compared them to each other reannotated sequence. For comparison, we also enterotoxigenic (ETEC) same ST23 lineage. Phylogenetic analysis were more...
Abstract This study focused on the use of antibiotics small, medium and commercial-sized dairy farms in central region Zambia its relationship to antibiotic resistance Escherichia coli . A stratified random sample 104 was studied, representing approximately 20% all region. On each farm, faecal samples were collected from a animals standardised questionnaire usage completed. An E. isolate obtained 98.67% (371/376) sampled tested for six classes antibiotics. The estimated prevalence across...
Gastrointestinal (GI) infections in sheep have significant implications for animal health, welfare and productivity, as well being a source of zoonotic pathogens. Interactions between pathogens epithelial cells at the mucosal surface play key role determining outcome GI infections; however, inaccessibility tract vivo significantly limits ability to study such interactions detail. We therefore developed ovine organoids representing physiologically important gastric intestinal sites infection,...