Gordon Dougan
A5115010691- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Vibrio bacteria research studies
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Clostridium difficile and Clostridium perfringens research
- Cancer Research and Treatments
- Genomics and Phylogenetic Studies
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- Probiotics and Fermented Foods
- Bacterial Infections and Vaccines
- Gut microbiota and health
- Immune Cell Function and Interaction
- Streptococcal Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Aquaculture disease management and microbiota
- Immunotherapy and Immune Responses
- RNA and protein synthesis mechanisms
- Nanoplatforms for cancer theranostics
- Pneumonia and Respiratory Infections
- Monoclonal and Polyclonal Antibodies Research
University of Cambridge
2016-2025
Wellcome Sanger Institute
2016-2025
Cambridge School
2020-2023
Bridge University
2023
Wellcome Trust
1990-2022
MRC Epidemiology Unit
2021-2022
NIHR Cambridge Biomedical Research Centre
2022
Boston Biomedical (United States)
2022
Addenbrooke's Hospital
2007-2021
Manipal Academy of Higher Education
2021
MicroRNAs are a class of small RNAs that increasingly being recognized as important regulators gene expression. Although hundreds microRNAs present in the mammalian genome, genetic studies addressing their physiological roles at an early stage. We have shown mice deficient for bic/microRNA-155 immunodeficient and display increased lung airway remodeling. demonstrate requirement function B T lymphocytes dendritic cells. Transcriptome analysis bic/microRNA-155-deficient CD4+ cells identified...
The Gram-negative bacterium Yersinia pestis is the causative agent of systemic invasive infectious disease classically referred to as plague, and has been responsible for three human pandemics: Justinian plague (sixth eighth centuries), Black Death (fourteenth nineteenth centuries) modern (nineteenth century present day). recent identification strains resistant multiple drugs potential use Y. an biological warfare mean that still poses a threat health. Here we report complete genome sequence...
Most mucosal surfaces of the mammalian body are colonized by microbial communities (“microbiota”). A high density commensal microbiota inhabits intestine and shields from infection (“colonization resistance”). The virulence strategies allowing enteropathogenic bacteria to successfully compete with overcome colonization resistance poorly understood. Here, we investigated manipulation intestinal bacterium Salmonella enterica subspecies 1 serovar Typhimurium (S. Tm) in a mouse colitis model:...
Abstract The SARS-CoV-2 Omicron BA.1 variant emerged in 2021 1 and has multiple mutations its spike protein 2 . Here we show that the of a higher affinity for ACE2 compared with Delta, marked change antigenicity increases Omicron’s evasion therapeutic monoclonal vaccine-elicited polyclonal neutralizing antibodies after two doses. mRNA vaccination as third vaccine dose rescues broadens neutralization. Importantly, antiviral drugs remdesivir molnupiravir retain efficacy against BA.1....
Epidemiological studies of the naturally transformable bacterial pathogen Streptococcus pneumoniae have previously been confounded by high rates recombination. Sequencing 240 isolates PMEN1 (Spain(23F)-1) multidrug-resistant lineage enabled base substitutions to be distinguished from polymorphisms arising through horizontal sequence transfer. More than 700 recombinations were detected, with genes encoding major antigens frequently affected. Among these 10 capsule-switching events, one which...
CD4(+)CD25(+) regulatory T (T(R)) cells can inhibit a variety of autoimmune and inflammatory diseases, but the precise mechanisms by which they suppress immune responses in vivo remain unresolved. Here, we have used Helicobacter hepaticus infection cell-reconstituted recombination-activating gene (RAG)(-/-) mice as model to study ability T(R) bacterially triggered intestinal inflammation. H. elicited both cell-mediated cell-independent inflammation, were inhibited adoptively transferred...
Abstract Although two-dose mRNA vaccination provides excellent protection against SARS-CoV-2, there is little information about vaccine efficacy variants of concern (VOC) in individuals above eighty years age 1 . Here we analysed immune responses following with the BNT162b2 2 elderly participants and younger healthcare workers. Serum neutralization levels binding IgG or IgA after first dose were lower older individuals, a marked drop over old. Sera from showed potency B.1.1.7 (Alpha),...
The gut microbiota is thought to play a key role in the development of inflammatory bowel diseases Crohn's disease (CD) and ulcerative colitis (UC). Shifts composition resident bacteria have been postulated drive chronic inflammation seen both (the "dysbiosis" hypothesis). We therefore specifically sought compare mucosa-associated from inflamed non-inflamed sites colon CD UC patients that non-IBD controls detect disease-specific profiles. Paired mucosal biopsies intestinal tissue 6 (n = 12)...
Isolates of methicillin-resistant Staphylococcus aureus (MRSA) belonging to a single lineage are often indistinguishable by means current typing techniques. Whole-genome sequencing may provide improved resolution define transmission pathways and characterize outbreaks.
Salmonella enterica subspecies is traditionally subdivided into serovars by serological and nutritional characteristics. We used Multilocus Sequence Typing (MLST) to assign 4,257 isolates from 554 1092 sequence types (STs). The majority of the many STs were grouped 138 genetically closely related clusters called eBurstGroups (eBGs). Many eBGs correspond a serovar, for example most Typhimurium are in eBG1 Enteritidis eBG4, but contained more than one serovar. Furthermore, polyphyletic...
Very high-throughput sequencing technologies need to be matched by functional studies if we are make full use of the current explosion in genome sequences. We have generated a very large bacterial mutant pool, consisting an estimated 1.1 million transposon mutants and used genomic DNA from this Illumina nucleotide prime sequence into adjacent target DNA. With method, which called TraDIS ( tra nsposon d irected i nsertion-site s equencing), been able map 370,000 unique insertion sites...
Antibiotic resistance is a major problem in Salmonella enterica serovar Typhi, the causative agent of typhoid. Multidrug-resistant (MDR) isolates are prevalent parts Asia and Africa often associated with dominant H58 haplotype. Reduced susceptibility to fluoroquinolones also widespread, sporadic cases third-generation cephalosporins or azithromycin have been reported. Here, we report first large-scale emergence spread novel S Typhi clone harboring three first-line drugs (chloramphenicol,...
Whereas most nontyphoidal Salmonella (NTS) are associated with gastroenteritis, there has been a dramatic increase in reports of NTS-associated invasive disease sub-Saharan Africa. enterica serovar Typhimurium isolates responsible for significant proportion the reported NTS this region. Multilocus sequence analysis S . from Malawi and Kenya identified dominant type, designated ST313, which currently is rarely outside Whole-genome sequencing multiple drug resistant (MDR) ST313 isolate,...
Relapsing C. difficile disease in humans is linked to a pathological imbalance within the intestinal microbiota, termed dysbiosis, which remains poorly understood. We show that mice infected with epidemic (genotype 027/BI) develop highly contagious, chronic and persistent dysbiosis characterized by distinct, simplified microbiota containing opportunistic pathogens altered metabolite production. Chronic 027/BI infection was refractory vancomycin treatment leading relapsing disease. In...