A5082537844- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Clostridium difficile and Clostridium perfringens research
- Streptococcal Infections and Treatments
- Nosocomial Infections in ICU
- Antibiotic Use and Resistance
- Mycobacterium research and diagnosis
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Pneumonia and Respiratory Infections
- Infective Endocarditis Diagnosis and Management
- COVID-19 Clinical Research Studies
- COVID-19 epidemiological studies
- Urinary Tract Infections Management
- Microscopic Colitis
- Central Venous Catheters and Hemodialysis
- Escherichia coli research studies
- Bacterial biofilms and quorum sensing
- Neonatal and Maternal Infections
- Infection Control in Healthcare
- Infectious Diseases and Tuberculosis
- Salmonella and Campylobacter epidemiology
- Genomics and Phylogenetic Studies
- Antibiotics Pharmacokinetics and Efficacy
Cambridge University Hospitals NHS Foundation Trust
2016-2025
Addenbrooke's Hospital
2014-2024
University Hospitals Bristol and Weston NHS Foundation Trust
2024
British Society for Antimicrobial Chemotherapy
2012-2024
UK Health Security Agency
2022-2024
Elmhurst College
2024
Wesley Hospital
2024
The University of Queensland
2024
University Hospitals Bristol NHS Foundation Trust
2024
The White House
2024
Isolates of methicillin-resistant Staphylococcus aureus (MRSA) belonging to a single lineage are often indistinguishable by means current typing techniques. Whole-genome sequencing may provide improved resolution define transmission pathways and characterize outbreaks.
The emergence of meticillin-resistant Staphylococcus aureus (MRSA) that can persist in the community and replace existing hospital-adapted lineages MRSA means it is necessary to understand transmission dynamics terms hospitals as one entity. We assessed use whole-genome sequencing enhance detection between these settings.We studied a putative outbreak on special care baby unit (SCBU) at National Health Service Foundation Trust Cambridge, UK. used validate expand findings from an...
Significant differences exist in the availability of healthcare worker (HCW) SARS-CoV-2 testing between countries, and existing programmes focus on screening symptomatic rather than asymptomatic staff. Over a 3 week period (April 2020), 1032 HCWs were screened for large UK teaching hospital. Symptomatic staff household contacts additionally tested. Real-time RT-PCR was used to detect viral RNA from throat+nose self-swab. 3% group tested positive SARS-CoV-2. 17/30 (57%) truly...
The burden and influence of health-care associated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections is unknown. We aimed to examine the use rapid SARS-CoV-2 sequencing combined with detailed epidemiological analysis investigate inform infection control measures.
Pandemic COVID-19 caused by the coronavirus SARS-CoV-2 has a high incidence of patients with severe acute respiratory syndrome (SARS). Many these require admission to an intensive care unit (ICU) for invasive ventilation and are at significant risk developing secondary, ventilator-associated pneumonia (VAP).
Escherichia coli associated with urinary tract infections and bacteremia has been intensively investigated, including recent work focusing on the virulent, globally disseminated, multidrug-resistant lineage ST131. To contextualize ST131 within broader E. population disease, we used genomics to analyze a systematic 11-yr hospital-based survey of using isolates collected from across England by British Society for Antimicrobial Chemotherapy Cambridge University Hospitals NHS Foundation Trust....
The increasing prevalence of E. coli bloodstream infections is a serious public health problem. We used genomic epidemiology in One Health study conducted the East England to examine putative sources associated with human disease. from 1,517 patients were compared 431 isolates livestock farms and meat. Livestock-associated genetically distinct populations based on core genome accessory analyses. Identical antimicrobial resistance genes found isolates, but there was limited overlap mobile...
The major human bacterial pathogen
<h3>Importance</h3> The latest generation of benchtop DNA sequencing platforms can provide an accurate whole-genome sequence (WGS) for a broad range bacteria in less than day. These could be used to more effectively contain the spread multidrug-resistant pathogens. <h3>Objective</h3> To compare WGS with standard clinical microbiology practice investigation nosocomial outbreaks caused by bacteria, identification genetic determinants antimicrobial resistance, and typing other clinically...
Longitudinal genomic and epidemiological surveillance of methicillin-resistant Staphylococcus aureus in the UK reveals extensive transmission hospitals community.
Whole-genome sequencing (WGS) can be used in genomic epidemiology investigations to confirm or refute outbreaks of bacterial pathogens, and support targeted efficient infection control interventions. We aimed define a genetic relatedness cutoff, quantified as number single-nucleotide polymorphisms (SNP), for meticillin-resistant Staphylococcus aureus (MRSA), above which recent (ie, within 6 months) patient-to-patient transmission could ruled out.We did retrospective epidemiological analysis...
Vancomycin-resistant Enterococcus faecium (VREfm) is a leading cause of nosocomial infection. Here, we describe the utility whole-genome sequencing in defining VREfm transmission.
The current paradigm for hospital outbreak detection and investigation is based on methodology first developed over 150 years ago. Daily surveillance to detect patients positive pathogens of particular importance nosocomial infection supported by epidemiological determine their relationship in time place, identify any other factor that could link them. antibiotic resistance pattern commonly used as a surrogate bacterial relatedness, although this lacks sensitivity specificity. Typing may be...
The rise in rates of human infection caused by vancomycin-resistant Enterococcus faecium (VREfm) strains between 1988 to the 2000s Europe was suggested be associated with acquisition from livestock. As a result, European Union banned use glycopeptide drug avoparcin as growth promoter livestock feed. While some studies reported decrease VREfm livestock, others no reduction. Here, we report first prevalence survey UK since 2003 and large-scale study using whole-genome sequencing investigate...
HighlightsCombined rapid antibody + nucleic acid detection correctly diagnoses SARS-CoV-2Rapid tests detect immune responses against SARS-CoV-2 bearing D614GRapid do not cross-react with antibodies to seasonal CoVFalse positivity in finger prick blood can be very lowSummaryRapid COVID-19 diagnosis the hospital is essential, although this complicated by 30%–50% of nose/throat swabs being negative amplification testing (NAAT). Furthermore, D614G spike mutant dominates pandemic and it unclear...
BackgroundDNA sequencing could become an alternative to in vitro antibiotic susceptibility testing (AST) methods for determining resistance by detecting genetic determinants associated with decreased susceptibility. Here, we aimed assess and improve the accuracy of determination from Enterococcus faecium genomes diagnosis surveillance purposes.MethodsIn this retrospective diagnostic study, first conducted a literature search PubMed on Jan 14, 2021, compile catalogue genes mutations...