A5028799787- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Vibrio bacteria research studies
- Antibiotic Use and Resistance
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial biofilms and quorum sensing
- Streptococcal Infections and Treatments
- Enterobacteriaceae and Cronobacter Research
- Antibiotics Pharmacokinetics and Efficacy
- Escherichia coli research studies
- Infective Endocarditis Diagnosis and Management
- Genomics and Phylogenetic Studies
- Clostridium difficile and Clostridium perfringens research
- Salmonella and Campylobacter epidemiology
- Neonatal and Maternal Infections
- Antifungal resistance and susceptibility
- Amoebic Infections and Treatments
- Infections and bacterial resistance
- Viral gastroenteritis research and epidemiology
- Fungal Infections and Studies
- Pneumonia and Respiratory Infections
- Tuberculosis Research and Epidemiology
- Bacterial Infections and Vaccines
- Parasitic Infections and Diagnostics
Singapore General Hospital
2016-2025
Duke-NUS Medical School
2019-2024
National University of Singapore
2007-2024
SingHealth
2022-2024
National Centre for Infectious Diseases
2020
National University Hospital
2008-2020
Nanyang Technological University
2020
Level (Czechia)
2020
DNA Diagnostic (Denmark)
2017
Genome Institute of Singapore
2014
Severe liver abscess infections caused by hypervirulent clonal-group CG23 Klebsiella pneumoniae have been increasingly reported since the mid-1980s. Strains typically possess several virulence factors including an integrative, conjugative element ICEKp encoding siderophore yersiniabactin and genotoxin colibactin. Here we investigate CG23's evolutionary history, showing deep-branching sublineages associated with distinct acquisitions. Over 80% of isolates belong to sublineage CG23-I, which...
BackgroundThe emerging fungal pathogen Candida auris poses a serious threat to global public health due its worldwide distribution, multidrug resistance, high transmissibility, propensity cause outbreaks, and mortality. We aimed characterise three unusual C isolates detected in Singapore, determine whether they constitute novel clade distinct from all previously known clades (I–V).MethodsIn this genotypic phenotypic study, we characterised clinical isolates, which were cultured...
Serotype K1 Klebsiella pneumoniae with multilocus sequence type 23 (ST23) has been strongly associated liver abscess in Taiwan. Few data regarding the strain types and virulence of this serotype from other Asian countries are available. K. strains isolated stool samples subjects hospitalized Hong Kong, Singapore, Taiwan hospitals were examined. Forty-seven isolates identified: 26 21 samples. MLST revealed 7 types: 85.1% (40 47 isolates) belonged to ST23, 1 isolate ST163 (a single-locus...
There are currently no standardized phenotypic methods for the screening and detection of AmpC enzymes. This study aimed to evaluate different detect enzymes in Escherichia coli, Klebsiella spp., Proteus comparing results from two disk-based an agar dilution method. activity was determined 255 clinical isolates by use a three-dimensional enzyme assay combined with multiplex PCR plasmid-borne ampC genes. These were compared against inhibitor using various combinations cefpodoxime cefoxitin as...
ABSTRACT Wastewater discharged from clinical isolation and general wards at two hospitals in Singapore was examined to determine the emerging trends of antibiotic resistance (AR). We quantified concentrations 12 compounds by analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS), antibiotic-resistant bacteria (ARB), class 1 integrase gene ( intI1 ), 16 genes (ARGs) that confer 10 different clinically relevant antibiotics. A subset 119 isolates were phylogenetically...
The dissemination of antimicrobial resistance (AMR) is an escalating problem and a threat to public health. Comparative metagenomics was used investigate the occurrence antibiotic resistant genes (ARGs) in wastewater urban surface water environments Singapore. Hospital municipal (
In Klebsiella pneumoniae liver abscess (KP-LA), K. pneumonia e K2 is the most frequently isolated serotype after K1, but this has been much less studied. present study, molecular types sequences type (MLST) of isolates from three different regions in Asia were identified and virulence these was investigated. Eight MLSTs found among 26 (ST 65, 66, 86, 373, 374, 375, 380, 434). There two major MLST groups, ST-65-like (42%) ST86-like (46%). No contained allS while all rmpA. The prevalence...
Streptococcus agalactiae (group B [GBS]) has not been described as a foodborne pathogen. However, in 2015, large outbreak of severe invasive sequence type (ST) 283 GBS infections adults epidemiologically linked to the consumption raw freshwater fish occurred Singapore. We attempted determine scale outbreak, define clinical spectrum disease, and link contaminated fish.Time-series analysis was performed on microbiology laboratory data. Food handlers fishmongers were screened for enteric...
ABSTRACT Among 177 carbapenemase-producing Gram-negative bacilli (108 KPC, 32 NDM, 11 IMP, 8 OXA-48, 4 OXA-181, 2 OXA-232, 5 IMI, VIM, and 3 SME producers), aztreonam-avibactam was active against all isolates except two NDM producers with elevated MICs of 8/4 16/4 mg/liter; ceftazidime-avibactam KPC-, IMI-, SME-, most OXA-48 group-producing (93%) but not metallo-β-lactamase producers. older contemporary antimicrobials, the were colistin, tigecycline, fosfomycin, overall susceptibilities 88%,...
Abstract Background Acinetobacter baumannii is an important nosocomial pathogen that has become increasingly resistant to multiple antibiotics. Genetic manipulation of MDR A. useful especially for defining the contribution each active efflux mechanism in multidrug resistance. Existing methods rely on use antibiotic selection marker and are not suited gene deletions. Results A tellurite-resistant ( sacB + , xylE ) suicide vector, pMo130-Tel R was created deleting adeFGH adeIJK operons two...
Since 2010, the incidence of carbapenem-resistant Enterobacteriaceae (CRE) has been increasing in Singapore. We analyzed clinical and molecular epidemiology CRE among adult inpatients Singapore.Quarterly unique subjects (per 100000 patient-days) with positive surveillance cultures for were estimated based on mandatory data submitted to National Public Health Laboratory by public hospitals between 2010 2015. CRE-positive prospectively recruited from 6 sector December 2013 April Subjects...
Gastrointestinal carriage of Klebsiella pneumoniae is a predisposing factor for liver abscess in several Asian countries. To determine whether hypervirulent K. the gut may be transmitted through food, we screened range raw and ready-to-eat retail food by culture recovered 21% (147 698) samples tested. Based on PCR, no isolates carried rmpA gene linked to community-acquired pyogenic abscess, providing evidence link between disease. However, phenotypic resistance multiple antibiotic classes...
The convergence of carbapenem-resistance and hypervirulence genes in Klebsiella pneumoniae has led to the emergence highly drug-resistant superbugs capable causing invasive disease. We analyzed 556 carbapenem-resistant K. isolates from patients Singapore hospitals during 2010-2015 discovered 18 7 also harbored features. All contained a closely related plasmid (pKPC2) harboring bla
Abstract Carbapenemase-producing Enterobacterales (CPE) infection control practices are based on the paradigm that detected carriers in hospital transmit to other patients who stay same ward. The role of plasmid-mediated transmission at population level remains largely unknown. In this retrospective cohort study over 4.7 years involving all multi-disciplinary public hospitals Singapore, we analysed 779 acquired CPE (1215 isolates) by clinical or surveillance cultures. 42.0% met putative...
Summary Background The emerging fungal pathogen Candida auris poses a serious threat to global public health due its worldwide distribution, multidrug-resistance, high transmissibility, propensity cause outbreaks and mortality rates. We report three C. isolates detected in Singapore, which are genetically distinct from all known clades (I-V) represent new clade (Clade VI). Methods Three epidemiologically unlinked clinical belonging the potential were whole-genome sequenced phenotypically...
ObjectiveTo compare the incidence of carbapenemase genes in Acinetobacter baumannii between two time periods.
Mucosa-associated invariant T (MAIT) cells are abundant antimicrobial in humans and recognize antigens derived from the microbial riboflavin biosynthetic pathway presented by MHC-Ib-related protein (MR1). However, mechanisms responsible for MAIT cell activity not fully understood, efficacy of these against antibiotic resistant bacteria has been explored. Here, we show that mediate MR1-restricted Escherichia coli clinical strains a manner dependent on cytolytic proteins but independent...
Hyperammonemia syndrome (HS) from Ureaplasma infection is typically reported in posttransplant recipients, particularly lung transplant. We describe a young woman with activated PI3K delta 2 who presented HS disseminated urealyticum septic arthritis. also performed literature review of Ureaplasma-associated nontransplant patients.
Summary We describe three zoonotic streptococcal soft tissue infections resulting from fresh seafood contact. One was a localized thumb infection with Streptococcus iniae in an immunocompetent healthy young male puncture wound crab pincer. The other two were cases of ascending upper limb cellulitis associated bacteraemia mastectomy patients. these caused by S. while the dysgalactiae subsp. , species that has not been previously described as cause infection. Hence when cleaning raw seafood,...
Acinetobacter baumannii is a major extensively drug-resistant lethal human nosocomial bacterium. However, the host innate immune mechanisms controlling A. are not well understood. Although viewed as an extracellular pathogen, can also invade and survive intracellularly. whether pathways sensing intracellular bacteria contribute to immunity against known. Here, we provide evidence for first time that antibacterial receptors Nod1 Nod2, their adaptor Rip2, play critical roles in clearance of by...
Carbapenem-resistant Enterobacteriaceae (CRE) can be mechanistically classified into carbapenemase-producing (CPE) and non-carbapenemase-producing carbapenem nonsusceptible (NCPCRE). We sought to investigate the effect of antecedent exposure as a risk factor for NCPCRE versus CPE. Among all patients with CRE colonization infection, we conducted case-control study comparing (cases) CPE (controls). The presence carbapenemases was investigated phenotypic tests followed by PCR predominant...