A5029776640- Antibiotic Resistance in Bacteria
- Bacterial Infections and Vaccines
- Genomics and Phylogenetic Studies
- Tuberculosis Research and Epidemiology
- Streptococcal Infections and Treatments
- Vibrio bacteria research studies
- Enterobacteriaceae and Cronobacter Research
- Bacterial Identification and Susceptibility Testing
- Pharmaceutical and Antibiotic Environmental Impacts
- Whipple's Disease and Interleukins
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial biofilms and quorum sensing
- Infective Endocarditis Diagnosis and Management
- Herpesvirus Infections and Treatments
- Mycobacterium research and diagnosis
- Infections and bacterial resistance
- Clostridium difficile and Clostridium perfringens research
- Salmonella and Campylobacter epidemiology
- Pneumonia and Respiratory Infections
- Vector-borne infectious diseases
- Bacteriophages and microbial interactions
- RNA regulation and disease
- Microscopic Colitis
- Yersinia bacterium, plague, ectoparasites research
- SARS-CoV-2 detection and testing
Kaiser Permanente
2025
Changi General Hospital
2017-2023
Ministry of Health
2013-2019
National University Health System
2013-2016
Institut de Médecine Tropicale du Service de Santé des Armées
2008
Hôpital de la Timone
2008
Centre National de la Recherche Scientifique
2005-2007
ABSTRACT Colistin and polymyxin B remain part of the last line antibiotics for multidrug-resistant Gram-negative bacteria, such as carbapenem-resistant Enterobacteriaceae . Current joint EUCAST-CLSI recommendations are broth microdilution (BMD) to be performed MIC testing colistin. Commercial susceptibility methods were evaluated compared against reference BMD, using a breakpoint ≤2 mg/liter both colistin B. Seventy-six included, which 21 mcr-1 positive (18 Escherichia coli isolates, 2...
Since 2010, the incidence of carbapenem-resistant Enterobacteriaceae (CRE) has been increasing in Singapore. We analyzed clinical and molecular epidemiology CRE among adult inpatients Singapore.Quarterly unique subjects (per 100000 patient-days) with positive surveillance cultures for were estimated based on mandatory data submitted to National Public Health Laboratory by public hospitals between 2010 2015. CRE-positive prospectively recruited from 6 sector December 2013 April Subjects...
Abstract Background IM CAB/RPV effectively maintained HIV viral suppression in prior randomized controlled trials. The study aimed to apply trial results a county population. secondary aim was characterize socioeconomic factors affecting implementation.Figure 1.Baseline vs. 6-Month Post Viral Load and CD4(Top) load between baseline, 3-month, 6-month, 12-month post-CAB/RPV switch. (Bottom) CD4 count switch Methods We conducted retrospective cohort including adult patients virologically...
Owing to gene transposition and plasmid conjugation, New Delhi metallo-β-lactamase (NDM) is typically identified among varied Enterobacteriaceae species STs. We used WGS characterize the chromosomal molecular epidemiology of NDM transmission involving four institutions in Singapore. Thirty-three isolates (collection years 2010–14) were sequenced using short-read sequencing-by-synthesis analysed. Long-read single molecule, real-time sequencing (SMRTS) was genetically a novel pSg1-NDM carried...
In this study, we describe the characterization of an infrequently encountered class A carbapenemase, IMI-1, from a clinical Enterobacter cloacae isolate. The isolate had high levels resistance to carbapenems but retained susceptibility expanded-spectrum cephalosporins. bla IMI-1 gene was chromosomally encoded. Detection producer highlights diversity carbapenemases in local setting.
The aim of this study was to determine the prevalence colistin-resistance gene (mcr-1) and antibiotic-susceptibility profile mcr-1 positive, colistin-resistant isolates in stool specimens patients attending a tertiary care hospital Singapore.201 diarrheal Changi General Hospital between May August 2017 were collected screened for presence by culture molecular methods. Antibiotic-susceptibility positive determined using polymyxin B colistin E-tests VITEK 2 system.We observed an unexpectedly...
Tropheryma whipplei is a poorly studied bacterium responsible for Whipple's disease. In this study, its susceptibility to doxycycline was investigated at transcriptional level using whole-genome DNA microarray.Exposure of T. the MIC (0.5 mg/L) induced antibiotic-specific primary expression profiles, while indirect effects were detected 10 x MIC. contrast what observed several microorganisms exposed antibiotics, heat-shock proteins not affected. Consistent with mode action translation...
Abstract Background The aim of this study was to analyze the genomic diversity several Tropheryma whipplei strains by microarray-based comparative hybridization. Fifteen clinical isolates originating from biopsy samples recovered different countries were compared with T. Twist strain. For each isolate, genes defined as either present or absent/divergent using GACK analysis software. Genomic changes then further characterized PCR and sequencing. Results results revealed a limited genetic...
Whole-genome sequencing was performed on 16 isolates of the carbapenemase-producing Enterobacter cloacae complex to determine flanking regions blaIMI-type genes. Phylogenetic analysis multilocus sequence typing (MLST) targets separated into 4 clusters. The genes were all found Xer-dependent integrative mobile elements (IMEX). IMEX 5 similar those described in Canada, while remainder novel. Five had lacking a resolvase and recombinase.
Abstract Background Community-acquired pneumonia (CAP) is one of the most common infectious diseases and a significant cause mortality morbidity globally. A microbial was not determined in sizable percentage patients with CAP; there are increasing data to suggest regional differences bacterial aetiology. We devised multiplex real-time PCR assay for detecting four microorganisms (Streptococcus pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae Burkholderia pseudomallei) relevance CAP...
A bstract : Rickettsiae survival implicates adaptation to different environmental conditions. We hypothesized that multiple copies of genes in bacteria with reduced genomes might account for such a process. Transcription spoT and sca paralogs was thus analyzed R. conorii felis .
Introduction: Bordetella trematum infection remains uncommon. More cases of bacteraemia are reported in recent years with the primary largely originating from skin and soft tissue sites. Yet, our understanding its virulence, antibiotic susceptibility profile treatment is still limited. Case presentation: We report first case B. a left-sided empyema. An 87-year old lady past medical history ischemic heart disease, diabetes mellitus complicated by nephropathy locally advanced left breast...