A5100767122- Pharmaceutical and Antibiotic Environmental Impacts
- SARS-CoV-2 detection and testing
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Antibiotic Resistance in Bacteria
- Liver Disease Diagnosis and Treatment
- Lysosomal Storage Disorders Research
- SARS-CoV-2 and COVID-19 Research
- Infective Endocarditis Diagnosis and Management
- Advanced biosensing and bioanalysis techniques
- Pituitary Gland Disorders and Treatments
- Meningioma and schwannoma management
- Peroxisome Proliferator-Activated Receptors
- Cancer-related molecular mechanisms research
- Metabolism and Genetic Disorders
- Urinary Bladder and Prostate Research
- Antibiotic Use and Resistance
- Biosensors and Analytical Detection
- Cancer, Hypoxia, and Metabolism
- Glioma Diagnosis and Treatment
- Antimicrobial Resistance in Staphylococcus
- Adipose Tissue and Metabolism
- Hepatitis Viruses Studies and Epidemiology
- Bacterial Identification and Susceptibility Testing
- Viral gastroenteritis research and epidemiology
Southern Medical University
2018-2025
Shandong University
2025
Nanfang Hospital
2018-2025
First Hospital of Lanzhou University
2010-2025
Ministry of Education of the People's Republic of China
2024-2025
China Jiliang University
2025
Guangdong Provincial Center for Disease Control and Prevention
2025
Nankai University
2025
Gansu University of Traditional Chinese Medicine
2025
Shanghai Jiao Tong University
2019-2024
Wastewater surveillance has emerged as a useful tool in the public health response to COVID-19 pandemic. While wastewater been applied at various scales monitor population-level dynamics, there is need for quantitative metrics interpret data context of trends. 24-hour composite samples were collected from March 2020 through May 2021 Massachusetts treatment plant and SARS-CoV-2 RNA concentrations measured using RT-qPCR. The relationship between copy numbers gene fragments clinical cases...
Sphingosine 1-phosphate (S1P) is a platelet-derived sphingolipid that binds to S1P 1 (EDG-1) receptors and activates the endothelial isoform of NO synthase (eNOS). polypeptide growth factor vascular (VEGF) act independently modulate angiogenesis activate eNOS. In these studies, we explored cross-talk between VEGF signaling pathways. When cultured bovine aortic cells were treated with (10 ng/ml), expression protein mRNA increased by ≈4-fold. up-regulation was seen within 30 min addition...
ABSTRACT Wastewater discharged from clinical isolation and general wards at two hospitals in Singapore was examined to determine the emerging trends of antibiotic resistance (AR). We quantified concentrations 12 compounds by analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS), antibiotic-resistant bacteria (ARB), class 1 integrase gene ( intI1 ), 16 genes (ARGs) that confer 10 different clinically relevant antibiotics. A subset 119 isolates were phylogenetically...
The dissemination of antimicrobial resistance (AMR) is an escalating problem and a threat to public health. Comparative metagenomics was used investigate the occurrence antibiotic resistant genes (ARGs) in wastewater urban surface water environments Singapore. Hospital municipal (
Peroxisomes are essential organelles for the specialized oxidation of a wide variety fatty acids, but they also able to degrade acids that typically handled by mitochondria. Using combination pharmacological inhibition and clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 genome editing technology simultaneously manipulate peroxisomal mitochondrial acid β-oxidation (FAO) in HEK-293 cells, we identified players metabolic crosstalk between these...
Abstract To study whether TGF-β1/IL-11/MEK/ERK (TIME) signaling mediates senescence-associated pulmonary fibrosis (SAPF) in Bmi-1- deficient ( Bmi-1 −/− ) mice and determines the major downstream mediator of crosstalk between p16 INK4a reactive oxygen species that regulates SAPF, phenotypes were compared among 7-week-old double-knockout, N -acetylcysteine (NAC)-treated , wild-type mice. Pulmonary fibroblasts alveolar type II epithelial (AT2) cells used for experiments. Human tissues tested Ι...
The critical need for surveillance of SARS-CoV-2 variants concern has prompted the development methods that can track in wastewater. Here, we develop and present an open-source method based on allele-specific RT-qPCR (AS RT-qPCR) detects quantifies B.1.1.7 variant, targeting spike protein mutations at three independent genomic loci are highly predictive (HV69/70del, Y144del, A570D). Our assays reliably detect quantify low levels with cross-reactivity, variant proportions down to 1% a...
Reclaimed water provides a supply alternative to address problems of scarcity in urbanized cities with high living densities and limited natural resources. In this study, wastewater metagenomes from 6 stages treatment plant (WWTP) integrating conventional membrane bioreactor (MBR) were evaluated for diversity antibiotic resistance genes (ARGs) bacteria, relative abundance class 1 integron integrases (intl1). ARGs confering 12 classes antibiotics (ARG types) persisted through the stages,...
Growth-factor-receptor-binding protein 2 (GRB2) is a non-enzymatic adaptor that plays pivotal role in precisely regulated signaling cascades from cell surface receptors to cellular responses, including transduction and gene expression. GRB2 binds numerous target molecules, thereby modulating complex network with diverse functions. The structural characteristics of are essential for its functionality, as multiple domains interaction mechanisms underpin biology. typical pathway involving...
The progression of osteoarthritis (OA) is closely linked to synovial inflammation caused by an imbalance between M1 and M2 macrophages. To tackle this problem, we developed a liposome responsive reactive oxygen species (ROS), modified with folic acid ligands target M1-polarized macrophages, loaded the anti-inflammatory agent dimethyl fumarate (DMF). This liposome-based drug delivery system was designed reprogram macrophage phenotype remodel inflammatory microenvironment in joint cavity...
The aim of the present study was to determine effect long non‑coding RNA (lncRNA) bladder cancer‑associated transcript 1 (BLACAT1) in chemoresistance non‑small cell lung cancer (NSCLC) cells. Expression lncRNA BLACAT1, microRNA (miR)‑17, autophagy‑related protein 7 (ATG7), multidrug‑resistance (MRP1), and autophagy‑associated proteins light chain 3 (LC3)‑II/LC3‑I Beclin were detected using reverse transcription‑quantitative polymerase reaction western blot analysis. Cell viability determined...
Antibiotic pollutions in the aquatic environment have attracted widespread attention due to their ubiquitous distribution and antibacterial properties. The occurrence, distribution, ecological risk assessment of 17 common antibiotics this study were preformed a vital drinking water source represented as river-reservoir system South China. In general, 15 detected at least once watershed, with total concentrations samples ranging from 193.6 863.3 ng/L 115.1 278.2 μg/kg sediment samples. For...
Antibiotic-associated diarrhea (AAD) affects a significant proportion of patients receiving antibiotics. We sought to understand if differences in the gut microbiome would influence development AAD. administered 3-day course amoxicillin-clavulanate 30 healthy adult volunteers, and analyzed their stool microbiome, using 16S rRNA gene sequencing, at baseline up 4 weeks post antibiotic administration. Lower levels Ruminococcaceae were significantly consistently observed from until day 7...
Arboviruses are viral pathogens transmitted by blood-borne vectors that impose a great social and economic burden globally. Most clinical surveillance of arbovirus outbreaks underestimates the true prevalence as large proportion cases exhibit no or only mild symptoms (i.e., subclinical). Reports urinary shedding several arboviruses such Dengue (DENV), yellow fever (YFV), Zika (ZIKV) viruses suggest possibility utilizing wastewater to assess arboviral outbreaks. To determine feasibility...
On November 26, 2021, the B.1.1.529 COVID-19 variant was classified as Omicron of concern (VOC). Reports higher transmissibility and potential immune evasion triggered flight bans heightened health control measures across world to stem its distribution. Wastewater-based surveillance has demonstrated be a useful complement for clinical community-based tracking SARS-CoV-2 variants. Using design principles our previous assays that detect variants (Alpha Delta), we developed an allele-specific...
Wastewater-based surveillance has been widely used as a non-intrusive tool to monitor population-level transmission of COVID-19. Although various approaches are available concentrate viruses from wastewater samples, scalable methods remain limited. Here, we sought identify and evaluate SARS-CoV-2 virus concentration protocols for high-throughput testing. A total twelve polyethylene glycol (PEG) precipitation four ultrafiltration-based were evaluated across two phases. The first phase...