A5058660780- Salmonella and Campylobacter epidemiology
- Brucella: diagnosis, epidemiology, treatment
- Viral gastroenteritis research and epidemiology
- Trace Elements in Health
- Escherichia coli research studies
- Mycobacterium research and diagnosis
- Microbial infections and disease research
- Antibiotic Resistance in Bacteria
- Tuberculosis Research and Epidemiology
- Burkholderia infections and melioidosis
- Animal Virus Infections Studies
- Veterinary medicine and infectious diseases
- Parasitic Infections and Diagnostics
- Aquaculture disease management and microbiota
- Bacteriophages and microbial interactions
- Galectins and Cancer Biology
- Antimicrobial Peptides and Activities
- Animal Nutrition and Physiology
- Clostridium difficile and Clostridium perfringens research
- Iron Metabolism and Disorders
- Vibrio bacteria research studies
- Animal Disease Management and Epidemiology
- Listeria monocytogenes in Food Safety
- Animal Diversity and Health Studies
- Gut microbiota and health
Istituto Superiore di Sanità
2013-2022
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"
2016-2020
University of Turin
2020
Ministry of Agriculture and Rural Development
2016
Polytechnic University of Turin
2014
Park University
2008
Agricultural Research Service
1997-2006
United States Department of Agriculture
2006
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche
1998-2004
University of Perugia
1998-2000
ABSTRACT Pseudomonas aeruginosa is a leading cause of hospital-acquired pneumonia and chronic lung infections in cystic fibrosis patients. Iron essential for bacterial growth, P. expresses multiple iron uptake systems, whose role infection deserves further investigation. Fe 3+ systems include the pyoverdine pyochelin siderophores two heme uptake, all which are dependent on TonB energy transducer. also has FeoB transporter 2+ acquisition. To assess roles individual infection, single double...
To investigate the relevance of zinc in host-pathogen interactions, we have constructed Salmonella enterica mutant strains which znuA gene, encodes periplasmic component ZnuABC high-affinity Zn2+ transporter, was deleted. This mutation does not alter ability to grow rich media but drastically reduces its multiply deprived zinc. In agreement with this phenotype, ZnuA accumulates only bacteria cultivated environments poor spite nearly millimolar intracellular concentration zinc, found that is...
Previous studies have suggested that P. aeruginosa possesses redundant zinc uptake systems. To identify uncharacterized transporters, we analyzed the genome-wide transcriptional responses of PA14 to restriction. This approach led identification an operon (zrmABCD) regulated by regulator Zur, encodes for a metallophore-mediated import system. includes genes TonB-dependent Outer Membrane Protein (ZrmA) and putative nicotianamine synthase (ZrmB). The simultaneous inactivation ZnuABC transporter...
The pathways ensuring the efficient uptake of zinc are crucial for ability bacteria to multiply in infected host. To better understand bacterial responses deficiency, we have investigated role periplasmic protein ZinT Salmonella enterica serovar Typhimurium. We found that zinT expression is regulated by Zur and parallels ZnuA, component transporter ZnuABC. Despite fact contributes growth media containing little zinc, disruption does not significantly affect virulence mice. became clear using...
Bovine tuberculosis (bTB) is an emerging disease among wild animals in many parts of the world. Wildlife reservoir hosts may thus represent a potential source infection for livestock and humans. We investigated role played by Sicilian black pig, autochthonous free- or semi-free-ranging domestic pig breed, as bTB area where prevalence cattle high. initially performed preliminary field study to assess occurrence such animals. sampled 119 pigs at abattoir found 6.7% 3.4% them be affected gross...
Salmonella enterica serovar Typhimurium is an important zoonotic gastrointestinal pathogen responsible for foodborne disease worldwide. It a successful enteric because it has developed virulence strategies allowing to survive in highly inflamed intestinal environment exploiting inflammation overcome colonization resistance provided by microbiota. In this study, we used piglets featuring intact microbiota, which naturally develop gastroenteritis, as model salmonellosis. We compared the...
On November 26, 2021, the B.1.1.529 COVID-19 variant was classified as Omicron of concern (VOC). Reports higher transmissibility and potential immune evasion triggered flight bans heightened health control measures across world to stem its distribution. Wastewater-based surveillance has demonstrated be a useful complement for clinical community-based tracking SARS-CoV-2 variants. Using design principles our previous assays that detect variants (Alpha Delta), we developed an allele-specific...
Zinc is an essential metal for cellular homeostasis and function in both eukaryotes prokaryotes. To acquire this nutrient, bacteria employ transporters characterized by different affinity the metal. Several studies have investigated role of high transporter ZnuABC bacterial response to zinc shortage, showing that has a key adapting starvation. In contrast, low importer ZupT been subject limited investigations. Here we show Salmonella strain lacking impaired its ability grow devoid...
The ability of a large number bacterial pathogens to multiply in the infected host and cause disease is dependent on their express high affinity zinc importers. In many bacteria, ZnuABC, transporter ABC family, plays central role process uptake poor environments, including tissues host. To initiate an investigation into relevance apparatus for Pseudomonas aeruginosa pathogenicity, we have generated znuA mutant PA14 strain. We found that this strain displays limited growth defect depleted...
Many of the most virulent strains Salmonella enterica produce two distinct Cu,Zn-superoxide dismutases (SodCI and SodCII). The bacteriophage-encoded SodCI enzyme makes greater contribution to virulence. We have performed a detailed comparison functional, structural, regulatory properties SodC enzymes. Here we demonstrate that SodCII differ with regard specific activity, protease resistance, metal affinity, peroxidative dimeric exhibiting superior stability activity. In particular, monomeric...
Abstract Background Antimicrobial resistant bacteria are emerging biological contaminants of the environment. In aquatic ecosystems, they originate mainly from hospitals, livestock manure and private households sewage water, which could contain antimicrobial agents microorganisms. Aeromonas spp. occur ubiquitously in environments cause disease fish. Motile aeromonads also associated with human gastrointestinal wound infections fish can act as a transmission route resistance (AMR) to humans....
Porcine Respiratory Disease Complex (PRDC) is a multifactorial syndrome that causes health problems in growing pigs and economic losses to farmers. The etiological factors involved can be bacteria, viruses, or mycoplasmas. However, environmental stressors associated with farm management influence the status of animal's health. role impact different microorganisms development disease complex, these are not fully understood. severity lesions consequence synergism combination factors. aim this...
Rifampin is one of the most potent and broad-spectrum antibiotics against bacterial pathogens. Its bactericidal activity due to its ability bind beta subunit DNA-dependent RNA polymerase encoded by rpoB gene. Mutations gene have been characterized in rifampin-resistant (Rif(r)) strains Escherichia coli Mycobacterium tuberculosis. The genetic bases Rif(r) Brucella spp. are still unknown. In present study, nucleotide sequences vaccine strain abortus RB51 20 clones derived our laboratory from...
BackgroundThe significance of behavioral neuroscience and the validity its animal models human pathology largely depend on possibility to replicate a given finding across different laboratories. Under present test housing conditions, this axiom fails resist challenge experimental validation. When several mouse strains are tested highly standardized batteries in laboratories, significant strain×lab interactions often detected. This limitation, predominantly due elevated within-group...
Bulgaria had been free from brucellosis since 1958, but during 2005-2007, a reemergence of human and animal disease was recorded. The this zoonosis in the country highlights importance maintaining an active surveillance system for infectious diseases that will require full cooperation between public health veterinary authorities.
Summary In this paper, a parasitological and serological study performed in three cows up to the fourth gestation is reported order clarify extent of vertical propagation and, secondly, which period recrudescence previous infection occurs. The selected for delivered healthy but congenitally infected calves first pregnancy. parasite was found, by biological tests Swiss mice, all placentas examined, during subsequent gestations, at calving. slaughtering, brains nine born clinically from as...
The present report describes a rare case of generalized bovine-type tuberculosis in slaughtered 4-year-old ewe discovered during routine surveillance at an abattoir. A postmortem examination revealed lesions the ewe's thoracic and abdominal cavities, ranging from encapsulated, mineralized foci to extensive, soft, caseous tissue. Lesions lungs, liver, lymph nodes were consistent with mycobacterial infection. histopathological detected granulomatous all tissue samples. presence Mycobacterium...
Salmonellosis in water buffalo (Bubalus bubalis) calves is a widespread disease characterized by severe gastrointestinal lesions, profuse diarrhea and dehydration, occasionally exhibiting systemic course. Several Salmonella serovars seem to be able infect buffalo, but isolates collected from this animal species have been poorly characterized. In the present study, prevalence of spp. affected lethal gastroenteritis was assessed, polyphasic characterization isolated strains S. Typhimurium...
Salmonella Typhimurium has been shown to be highly effective as antitumor agent. The aim of this study was investigate the tumor targeting efficacy and mechanism action a specific attenuated mutant strain (STM) devoid whole operon coding for high-affinity zinc transporter ZnuABC, which is required bacterial growth in environments poor conferring full virulence different Gram-negative pathogens.We showed that STM able penetrate replicate into cells vitro vivo models. subcutaneous...