A5007656010- Parasite Biology and Host Interactions
- Parasitic Diseases Research and Treatment
- Helminth infection and control
- Parasitic Infections and Diagnostics
- Parasites and Host Interactions
- Nematode management and characterization studies
- Coccidia and coccidiosis research
- Animal Disease Management and Epidemiology
- Parasitic infections in humans and animals
- Viral gastroenteritis research and epidemiology
- Toxoplasma gondii Research Studies
- Animal Virus Infections Studies
- Insects and Parasite Interactions
- Insect symbiosis and bacterial influences
- Protein Hydrolysis and Bioactive Peptides
- Protist diversity and phylogeny
- Virus-based gene therapy research
- T-cell and Retrovirus Studies
- Vector-Borne Animal Diseases
- Herpesvirus Infections and Treatments
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Pneumocystis jirovecii pneumonia detection and treatment
- Antimicrobial Peptides and Activities
Agricultural Research Service
2016-2025
United States Department of Agriculture
2011-2024
Beltsville Agricultural Research Center
2015-2024
Beltsville Human Nutrition Research Center
2023
United States Department of State
2020
Pennsylvania State University
2007
Cornell University
2007
University of Nebraska Medical Center
2007
Bhabha Atomic Research Centre
2005
CNR de la Résistance aux Antibiotiques
2001
Parasitic nematodes (roundworms) and platyhelminths (flatworms) cause debilitating chronic infections of humans animals, decimate crop production are a major impediment to socioeconomic development. Here we report broad comparative study 81 genomes parasitic non-parasitic worms. We have identified gene family births hundreds expanded families at key nodes in the phylogeny that relevant parasitism. Examples include modulate host immune responses, enable parasite migration though tissues or...
Richard Wilson and colleagues report the genome sequence of Trichinella spiralis, a food-borne parasitic nematode that diverged early in evolution phylum Nematoda. T. spiralis is most common cause human trichinellosis. Genome studies for Nematoda have been limited by focusing on comparisons involving Caenorhabditis elegans. We draft zoonotic parasite, which This an extant member clade phylum, enabling identification archetypical genes molecular signatures exclusive to nematodes. sequenced...
[Extract] Mitochondrial genomes have been used in numerous studies to investigate phylogenetic relationships among eukaryotes at many levels (e.g., Smith et al. 1993; Boore 1995; Boore, Lavrov, and Brown 1998). In recent years, the arrangement of genes mitochondrial genome has regarded as a powerful record historical (Boore 1999). Changes gene order are infrequent, even over considerable spans time 1999), unlikely exhibit homoplasy. Our research focused on between two groups human blood...
Parasitic nematodes of the genus Trichinella cause significant food-borne illness and occupy a unique evolutionary position at base phylum Nematoda, unlike free-living nematode Caenorhabditis elegans. Although forthcoming genome sequence spiralis can provide invaluable comparative information about biology, basic framework for understanding history is needed to maximize its utility. We therefore developed first robust comprehensive analysis phylogeny biogeographic using variation in three...
ABSTRACT A highly sensitive and specific method has been developed to reproducibly detect quantitate Toxoplasma gondii burden in animal tissue samples using T. ITS1-derived primers a fluorogenic probe via real-time PCR. Assay specificity was confirmed against panel of DNA from other common protozoa as well host tissue. This Toxo TaqMan assay able little 0.1 pg genomic DNA, which is equivalent 1 bradyzoite, dynamic range detection 100 ng fg DNA. Tissues experimentally infected mice pigs...
To identify genes mediating the antiproliferative action of interferon (IFN), two cDNA libraries were constructed with mRNA from IFN-treated and untreated human fibrosarcoma (HT1080) cells previously shown to be highly sensitive effects IFN. Differential screening these identified cloned sequences whose expression was either induced or repressed IFN treatment. Rescreening probes proliferating quiescent led identification one IFN-induced three IFN-repressed expressions also appeared modulated...
Lipid rafts are major structural components in plasma membranes that play critical roles many biological processes including virus infection. However, few reports have described the relationship between lipid and porcine rotavirus (PRV) In this study, we investigated whether or not locally high concentrations (3–5 fold) of cholesterol present required for PRV infection, further examined which stages infection process most affected. When cellular was depleted by methyl-β-cyclodextrin (MβCD),...
Parasitic worms have a remarkable ability to modulate host immune responses through several mechanisms including excreted/secreted proteins (ESP), yet the exact nature of these and their targets often remains elusive. Here, we performed mass spectrometry analyses ESP (TsESP) from larval adult stages pig whipworm Trichuris suis (Ts) identified ~350 proteins. Transcriptomic revealed large subsets differentially expressed genes in various life cycle parasite. Exposure bone marrow-derived...
Enzymatic amplification of expansion segment 5 sequences within domain IV the large subunit ribosomal DNA generated distinct results among geographical isolates Trichinella pseudospiralis from Russia, North America, and Australia both avian mammalian hosts. Discrete, multiple fragments ranging in approximate size 285 to 360 bp were observed each tested. Polymerase chain reaction performed on individual adult parasites isolate resulted that comparable those pooled genomic DNA. Sequence...