A5089784505- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- HIV Research and Treatment
- Immunotherapy and Immune Responses
- Escherichia coli research studies
- IL-33, ST2, and ILC Pathways
- HIV-related health complications and treatments
- Phagocytosis and Immune Regulation
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Hematopoietic Stem Cell Transplantation
- Viral Infections and Outbreaks Research
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Complement system in diseases
- Telomeres, Telomerase, and Senescence
- Poxvirus research and outbreaks
- Reproductive System and Pregnancy
- Nanoplatforms for cancer theranostics
- Bat Biology and Ecology Studies
- SARS-CoV-2 detection and testing
- Macrophage Migration Inhibitory Factor
- Galectins and Cancer Biology
- Acute Myeloid Leukemia Research
Tsinghua–Berkeley Shenzhen Institute
2020-2025
Tsinghua University
2020-2025
Karolinska Institutet
2013-2022
National University of Singapore
2016-2022
Karolinska University Hospital
2012-2021
Biology of Infection
2021
The University of Melbourne
2010-2013
Monash University
2006-2013
Burnet Institute
2006-2013
Mucosa-associated invariant T (MAIT) cells represent a large innate-like evolutionarily conserved antimicrobial T-cell subset in humans. MAIT recognize microbial riboflavin metabolites from range of microbes presented by MR1 molecules. are impaired several chronic diseases including HIV-1 infection, where they show signs exhaustion and decline numerically. Here, we examined the broader effector functions this context strategies to rescue their functions. Residual HIV-infected patients...
Innate-like, evolutionarily conserved MR1-restricted mucosa-associated invariant T (MAIT) cells represent a large antimicrobial T-cell subset in humans. Here, we investigate the development of these second trimester human fetal tissues. MAIT are rare and immature thymus, spleen mesenteric lymph nodes. In contrast, mature IL-18Rα+ CD8αα enriched small intestine, liver lung. Independently localization, express CD127 Ki67 vivo readily proliferate response to Escherichia coli vitro. Maturation...
Significance Mucosa-associated invariant T (MAIT) cells are a large subset of unconventional in humans, recognizing microbial riboflavin metabolites presented by the monomorphic MR1 molecule. The extraordinary level conservation and limited diversity riboflavin-derived antigens have suggested that MAIT homogeneous, their functional specialization has not been thoroughly investigated. Here, we show cell responses against two distinct biosynthesis-competent microorganisms display...
The female genital tract (FGT) mucosa is a critically important site for immune defense against microbes. Mucosal-associated invariant T (MAIT) cells are an innate-like T-cell population that recognizes microbial riboflavin metabolite antigens in MR1-dependent manner. role of MAIT the FGT unknown. Here, we found and MR1+ antigen-presenting were present upper lower FGT, with distinct tissue localization endometrium vs. cervix. from blood displayed phenotype expression interleukin (IL)-18Rα,...
Immune exhaustion is a hallmark of chronic viral infections. However, pathogen eradication can result in reinvigorated immune responses. Indeed, this was recently suggested for antigen‐specific CD8 + T cells and NK HCV‐infected patients receiving an interferon‐free treatment regimen. Mucosal‐associated invariant (MAIT) are evolutionarily conserved innate‐like effector cells. Here, we show that MAIT severely diminished frequency HCV‐infection, regard the most affected cell type peripheral...
Significance Mucosa-associated invariant T (MAIT) cells are unconventional innate-like recognizing microbial riboflavin metabolites presented by the monomorphic MR1 molecule. Here, we show that CD8 + CD4 − and subpopulations of human MAIT represent transcriptionally phenotypically discrete subsets with distinct functional profiles. Furthermore, cell receptor repertoire analysis, as well data based on fetal tissues, umbilical cord blood, culture systems indicate subset may derive from main...
Background. Human immunodeficiency virus (HIV)-infected patients on combination active antiretroviral therapy (cART) are at increased risk of age-related complications. We hypothesized that nucleos(t)ide reverse transcriptase inhibitors (NRTI) may contribute to accelerated aging in HIV-infected individuals cART via inhibition telomerase activity. Methods. Telomerase activity and telomere length (TL) were measured by quantitative polymerase chain reaction vitro activated peripheral blood...
Abstract Mucosa‐associated invariant T (MAIT) cells are unconventional lymphocytes defined by their innate‐like characteristics and broad antimicrobial responsiveness. Whether MAIT part of the tissue‐resident defense in oral mucosal barrier is unknown. Here, we found present buccal mucosa, with a tendency to cluster near basement membrane, located both epithelium underlying connective tissue. Overall cell levels were similar mucosa compared peripheral blood, contrast conventional that showed...
Mucosa-associated invariant T cells are a large and relatively recently described innate-like antimicrobial T-cell subset in humans. These recognize riboflavin metabolites from range of microbes presented by evolutionarily conserved major histocompatibility complex, class I-related molecules. Given the characteristics mucosa-associated novel type antigens they recognize, new methodology must be developed existing methods refined to allow comprehensive studies their role human immune defense...
The global outbreak of the monkeypox virus (MPXV) highlights need for rapid and cost-effective MPXV detection tools to effectively monitor control disease. Herein, we demonstrated a portable CRISPR-Cas-based system naked-eye MPXV. harnesses high selectivity CRISPR-Cas12 isothermal nucleic acid amplification potential recombinase polymerase amplification. It can detect both current circulating clade original clades. We reached limit (LoD) 22.4 aM (13.5 copies/µl) using microtiter plate...
Mucosal-associated invariant T (MAIT) cells are unconventional that recognize microbial riboflavin pathway metabolites presented by evolutionarily conserved MR1 molecules. We explored the human MAIT cell compartment across organ donor–matched blood, barrier, and lymphoid tissues. population size was donor dependent with distinct tissue compartmentalization patterns adaptations: Intestinal CD103 + resident an immunoregulatory CD39 high CD27 low profile, whereas expressing NCAM1/CD56 dominated...
Mucosal-associated invariant T (MAIT) cells are antimicrobial abundant in the gut, but mechanisms for their migration into tissues during inflammation poorly understood. Here, we used acute pediatric appendicitis (APA), a model of intestinal inflammation, to examine these mechanisms. MAIT were lower numbers circulation patients with APA enriched inflamed appendix increased production proinflammatory cytokines. Using patient-derived organoid (PDAO) model, found that circulating treated...
Mucosa-associated invariant T (MAIT) cells are abundant antimicrobial in humans and recognize antigens derived from the microbial riboflavin biosynthetic pathway presented by MHC-Ib-related protein (MR1). However, mechanisms responsible for MAIT cell activity not fully understood, efficacy of these against antibiotic resistant bacteria has been explored. Here, we show that mediate MR1-restricted Escherichia coli clinical strains a manner dependent on cytolytic proteins but independent...
Abstract Mucosa-associated invariant T (MAIT) cell loss in chronic HIV-1 infection is a significant insult to antimicrobial immune defenses. Here we investigate the response of MAIT cells during acute utilizing RV217 cohort with paired longitudinal pre- and post-infection samples. are activated expand blood mucosa coincident peak viremia, manner associated emerging microbial translocation. This followed by phase elevated function as viral replication controlled set-point level, later their...
Progress in our understanding of MR1-restricted mucosa-associated invariant T (MAIT) cells has raised interest harnessing these for immunotherapy. The innate-like response characteristics, abundance the blood, donor-unrestricted nature, and tropism tissues make MAIT suitable candidates adoptive cell transfer therapies. However, reliable methods tools to utilize such approaches are lacking. Here, we established methodology efficient expansion human culture with high purity yield, while...
Our understanding of human type 1 natural killer T (NKT) cells has been heavily dependent on studies from peripheral blood. These have identified two functionally distinct subsets defined by expression CD4, although it is widely believed that this underestimates the true number subsets. Two recent supporting view provided more detail about diversity NKT cells, but relied analysis blood had expanded in vitro prior to analysis. In study we extend those findings assessing heterogeneity CD4(+)...
NK cells lacking CD56 (CD56neg ) were first identified in chronic HIV-1 infection. However, CD56neg also exist healthy individuals, albeit significantly lower numbers. Here, we provide an extensive proteomic characterisation of human peripheral blood donors and compare them to their CD56dim CD56bright counterparts. Unbiased large-scale surface receptor profiling clustered as part the main cell compartment indicated overall -like phenotype. Total proteome analyses further confirmed similarity...
Summary The causes of multiple myeloma (MM) remain obscure and there are few known risk factors; however, natural killer T (NKT) cell abnormalities have been reported in patients with MM, therapeutic targeting NKT cells is promoted as a potential treatment. We characterized defects treated untreated MM determined the impact lenalidomide therapy on pool. Lenalidomide an immunomodulatory drug co-stimulatory effects vitro approved treatment for although its mode action that context not well...