Marcus Buggert
A5057116894- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- HIV Research and Treatment
- Immunotherapy and Immune Responses
- Long-Term Effects of COVID-19
- Cytomegalovirus and herpesvirus research
- Immunodeficiency and Autoimmune Disorders
- Immune responses and vaccinations
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- vaccines and immunoinformatics approaches
- Phagocytosis and Immune Regulation
- Single-cell and spatial transcriptomics
- Chronic Lymphocytic Leukemia Research
- IL-33, ST2, and ILC Pathways
- Monoclonal and Polyclonal Antibodies Research
- Dermatological and COVID-19 studies
- Immune cells in cancer
- SARS-CoV-2 detection and testing
- COVID-19 and Mental Health
- Gut microbiota and health
- HIV-related health complications and treatments
- Tryptophan and brain disorders
Karolinska Institutet
2016-2025
Integrated Cardio Metabolic Centre
2023-2025
Karolinska University Hospital
2015-2024
Svenska Örtmedicinska Institute
2017-2023
Lund University
2016-2023
Science for Life Laboratory
2023
National Bioinformatics Infrastructure Sweden
2023
University of Pennsylvania
2015-2022
University of Pennsylvania Health System
2019
Children's Hospital of Philadelphia
2019
Understanding innate immune responses in COVID-19 is important to decipher mechanisms of host and interpret disease pathogenesis. Natural killer (NK) cells are effector lymphocytes that respond acute viral infections but might also contribute immunopathology. Using 28-color flow cytometry, we here reveal strong NK cell activation across distinct subsets peripheral blood patients. This pattern was mirrored scRNA-seq signatures bronchoalveolar lavage from Unsupervised high-dimensional analysis...
Abstract The emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron (B.1.1.529) variant concern (VOC) has destabilized global efforts to control impact disease 2019 (COVID-19). Recent data have suggested that B.1.1.529 can readily infect people with naturally acquired or vaccine-induced immunity, facilitated in some cases by viral escape from antibodies neutralize ancestral SARS-CoV-2. However, appears be relatively uncommon such individuals, highlighting a...
CD8+ T cell exhaustion represents a major hallmark of chronic HIV infection. Two key transcription factors governing differentiation, T-bet and Eomesodermin (Eomes), have previously been shown in mice to differentially regulate part through direct modulation PD-1. Here, we examined the relationship between these expression several inhibitory receptors (PD-1, CD160, 2B4), functional characteristics memory differentiation cells treated The PD-1, 2B4 on total was elevated chronically infected...
Mucosa-associated invariant T (MAIT) cells represent a large innate-like evolutionarily conserved antimicrobial T-cell subset in humans. MAIT recognize microbial riboflavin metabolites from range of microbes presented by MR1 molecules. are impaired several chronic diseases including HIV-1 infection, where they show signs exhaustion and decline numerically. Here, we examined the broader effector functions this context strategies to rescue their functions. Residual HIV-infected patients...
MAIT cell activation and decline in blood are associated with COVID-19 severity, features that dynamically recover convalescence.
ABSTRACT SARS-CoV-2-specific memory T cells will likely prove critical for long-term immune protection against COVID-19. We systematically mapped the functional and phenotypic landscape of cell responses in a large cohort unexposed individuals as well exposed family members with acute or convalescent Acute phase displayed highly activated cytotoxic phenotype that correlated various clinical markers disease severity, whereas were polyfunctional stem-like phenotype. Importantly, detectable...
The transcription factor TOX is not exclusively linked to T cell exhaustion (see related Focus by Utzschneider and Kallies).
Unanswered questions remain about the contributions of T cell immunity to protective and dysfunctional responses in COVID-19.
Dendritic cells (DCs) and monocytes are crucial mediators of innate adaptive immune responses during viral infection, but misdirected by these may contribute to immunopathology. Here, we performed high-dimensional flow cytometry-analysis focusing on mononuclear phagocyte (MNP) lineages in SARS-CoV-2-infected patients with moderate severe COVID-19. We provide a deep comprehensive map the MNP landscape A redistribution monocyte subsets toward intermediate general decrease circulating DCs was...
T cells are critical in mediating the early control of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) breakthrough infection. However, it remains unknown whether memory can effectively cross-recognize new SARS-CoV-2 variants with a broad array mutations, such as emergent hypermutated BA.2.86 variant. Here, we report two separate cohorts, including healthy controls and individuals chronic lymphocytic leukemia, that spike-specific CD4
Humoral immunity is critical for viral control, but the identity and mechanisms regulating human antiviral B cells are unclear. Here, we characterized expressing T-bet analyzed their dynamics during infections. T-bet+ demonstrated an activated phenotype, a distinct transcriptional profile, were enriched expression of immunoglobulin isotypes IgG1 IgG3. expanded following yellow fever virus vaccinia vaccinations also early acute HIV infection. Viremic HIV-infected individuals maintained large...
Significance Mucosa-associated invariant T (MAIT) cells are unconventional innate-like recognizing microbial riboflavin metabolites presented by the monomorphic MR1 molecule. Here, we show that CD8 + CD4 − and subpopulations of human MAIT represent transcriptionally phenotypically discrete subsets with distinct functional profiles. Furthermore, cell receptor repertoire analysis, as well data based on fetal tissues, umbilical cord blood, culture systems indicate subset may derive from main...
In this study, we describe four patients from two unrelated families of different ethnicities with a primary immunodeficiency, predominantly manifesting as susceptibility to Epstein-Barr virus (EBV)–related diseases. Three presented EBV-associated Hodgkin’s lymphoma and hypogammaglobulinemia; one also had severe varicella infection. The fourth viral encephalitis during infancy. Homozygous frameshift or in-frame deletions in CD70 these abolished either surface expression binding its cognate...
Current paradigms of CD8 + T cell–mediated protection in HIV infection center almost exclusively on studies peripheral blood, which is thought to provide a window into immune activity at the predominant sites viral replication lymphoid tissues (LTs). Through extensive comparison thoracic duct lymph (TDL), and LTs different species, we show that many LT memory cells bear phenotypic, transcriptional, epigenetic signatures resident (T RMs ). Unlike their circulating counterparts blood or TDL,...
T follicular helper cells (Tfh), a subset of CD4+ cells, provide requisite help to B in the germinal centers (GC) lymphoid tissue. GC Tfh are identified by high expression chemokine receptor CXCR5 and inhibitory molecule PD-1. Although more accessible, blood contains lower frequencies CXCR5+ PD-1+ that have been termed circulating (cTfh). However, it remains unclear whether exit tissues populate this cTfh pool. To examine exiting we assessed phenotype present within major conduit efferent...
T follicular regulatory (Tfr) cells are a population of CD4+ that express cell markers and have been shown to suppress humoral immunity. However, the precise mechanisms location Tfr-mediated suppression in lymph node (LN) microenvironment unknown. Using highly multiplexed quantitative imaging functional assays, we examined spatial distribution, suppressive function, preferred interacting partners Tfr human mesenteric LNs. We find majority low levels PD-1 reside at border between zone B...
Elite control of HIV replication is linked to polyfunctional lymphoid CD8 + T cells that lack overt cytolytic activity and home B cell follicles.
Elimination of lymphoid tissue reservoirs is a key component HIV eradication strategies. CD8+ T cells play critical role in control HIV, but their functional attributes lymph nodes (LNs) remain unclear. Here, we show that memory, follicular CXCR5+, and HIV-specific from LNs do not manifest the properties cytolytic cells. While frequency CXCR5+ was strongly inversely associated with peripheral viremia, this association dependent on Moreover, poor activity LN linked to compartmentalized...
Tissue-resident memory CD8 + T cells in unexposed oropharyngeal lymphoid tissue exhibit specificity for SARS-CoV-2.
Significance Accumulating evidence shows that granulocytes are key modulators of the immune response to SARS-CoV-2 infection, and their dysregulation could significantly impact COVID-19 severity patient recovery after virus clearance. In present study, we identify selected traits in neutrophil, eosinophil, basophil subsets associated with peripheral protein profiles. Moreover, computational modeling indicates combined use phenotypic data laboratory measurements can effectively predict...