A5080628718- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Eosinophilic Esophagitis
- T-cell and B-cell Immunology
- Reproductive System and Pregnancy
- Asthma and respiratory diseases
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Inflammatory Bowel Disease
- Long-Term Effects of COVID-19
- Immune responses and vaccinations
- Autoimmune and Inflammatory Disorders Research
- Pregnancy and Medication Impact
- Immunotherapy and Immune Responses
- Single-cell and spatial transcriptomics
- Phagocytosis and Immune Regulation
- Pregnancy and preeclampsia studies
- Dermatology and Skin Diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Viral Infections and Vectors
- Mast cells and histamine
- Air Quality and Health Impacts
- Mycobacterium research and diagnosis
- Immune cells in cancer
- CAR-T cell therapy research
Karolinska Institutet
2016-2025
Karolinska University Hospital
2016-2025
Sachs' Children and Youth Hospital
2022
Stockholm South General Hospital
2022
Linköping University
2007-2020
National Center for Infectious Diseases
2014
Integrated Cardio Metabolic Centre
2014
University of Amsterdam
2011-2013
Amsterdam UMC Location University of Amsterdam
2011-2013
Cargotec (Poland)
2009
Tissue-resident memory T (Trm) cells form a heterogeneous population that provides localized protection against pathogens. Here, we identify CD49a as marker differentiates CD8+ Trm on compartmental and functional basis. In human skin epithelia, CD8+CD49a+ produced interferon-γ, whereas CD8+CD49a− interleukin-17 (IL-17). addition, from healthy rapidly induced the expression of effector molecules perforin granzyme B when stimulated with IL-15, thereby promoting strong cytotoxic response....
BackgroundActivation of the group 2 innate lymphoid cell (ILC2) population leads to production classical type cytokines, thus promoting immunity. Chemoattractant receptor-homologous molecule expressed on TH2 cells (CRTH2), a receptor for prostaglandin D2 (PGD2), is by human ILC2s. However, function CRTH2 in these unclear.ObjectivesWe sought determine role PGD2 and ILC2s compare it with that established ILC2 activators IL-25 IL-33.MethodsThe effects PGD2, IL-25, IL-33 migration, cytokine...
Although uterine macrophages are thought to play an important regulatory role at the maternal-fetal interface, their global gene expression profile is not known.Using micro-array comprising approximately 14,000 genes, pattern of human first trimester decidual CD14+ monocytes/macrophages was characterized and compared with corresponding cells in blood. Some key findings were confirmed by real time PCR or secreted protein. A unique intrinsic demonstrated. large number regulated genes...
In pregnancy, the decidua is infiltrated by leukocytes promoting fetal development without causing immunological rejection. Murine regulatory T (Treg) cells are known to be important immune regulators at this site. The aim of study was characterize phenotype and origin Treg determine quantitative relationship between Treg, T-helper type 1 (T(H)1), T(H)2, T(H)17 in first-trimester human decidua. Blood decidual CD4(+) from 18 healthy pregnant women were analyzed for expression Treg-cell...
Abstract Although NK cells are considered innate, recent studies in mice revealed the existence of a unique lineage hepatic CD49a+DX5− with adaptive-like features. Development this cell is, contrast to conventional cells, dependent on T-bet but not Eomes. In study, we describe identification T-bet+Eomes−CD49a+ subset readily detectable human liver, afferent or efferent venous peripheral blood. Human intrahepatic CD49a+ express killer Ig-like receptor and NKG2C, indicative having undergone...
MAIT cell activation and decline in blood are associated with COVID-19 severity, features that dynamically recover convalescence.
The impact of the microenvironment on innate lymphoid cell (ILC)-mediated immunity in humans remains largely unknown. Here we used full-length Smart-seq2 single-cell RNA-sequencing to unravel tissue-specific transcriptional profiles and heterogeneity CD127+ ILCs across four human tissues. Correlation analysis identified gene modules characterizing migratory properties tonsil blood ILCs, signatures tissue-residency, activation modified metabolism colon lung ILCs. Trajectory revealed potential...
Dendritic cells (DCs) and monocytes are crucial mediators of innate adaptive immune responses during viral infection, but misdirected by these may contribute to immunopathology. Here, we performed high-dimensional flow cytometry-analysis focusing on mononuclear phagocyte (MNP) lineages in SARS-CoV-2-infected patients with moderate severe COVID-19. We provide a deep comprehensive map the MNP landscape A redistribution monocyte subsets toward intermediate general decrease circulating DCs was...
Immune cell dysfunction within the tumor microenvironment (TME) undermines control of cancer progression. Established tumors contain phenotypically distinct, tumor-specific natural killer (NK) cells; however, temporal dynamics, mechanistic underpinning and functional significance NK compartment remains incompletely understood. Here, we use photo-labeling, combined with longitudinal transcriptomic cellular analyses, to interrogate fate intratumoral cells. We reveal that cells rapidly lose...
Human RORC+ lymphoid tissue inducer cells are part of a rapidly expanding family innate (ILC) that participate in and adaptive immune responses as well (re) modeling. The assessment potential role for lymphocyte-derived cytokines human homeostasis disease is hampered by poor characterization cell subsets lack knowledge on the distribution these adults. Here we show functionally distinct differentially produce IL-17a or IL-22. Both have an activated phenotype can be distinguished based...
CD4(+)CD25(high) regulatory T cells (Tregs) are implicated in the maintenance of murine pregnancy. However, reports regarding circulating Treg frequencies human pregnancy inconsistent, and functionality phenotype these have not been clarified. The aim this study was to determine frequency, phenotype, function Tregs second trimester influence progesterone 17beta-estradiol on frequency. Based expressions Foxp3, CD127, HLA-DR as determined by multicolor flow cytometry, we defined a proper...
BackgroundGroup 2 innate lymphoid cells (ILC2s) are involved in the initial phase of type inflammation and can amplify allergic immune responses by orchestrating other cells. Prostaglandin (PG) E2 is a bioactive lipid that plays protective roles lung, particularly during inflammation.ObjectiveWe set out to investigate how PGE2 regulates human ILC2 function.MethodsThe effects on proliferation intracellular cytokine transcription factor expression were assessed means flow cytometry. Cytokine...