A5020615799- Diabetes and associated disorders
- Pancreatic function and diabetes
- Diabetes Management and Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Digestive system and related health
- Dermatology and Skin Diseases
- Asthma and respiratory diseases
- Infant Nutrition and Health
- Immunotherapy and Immune Responses
- Animal health and immunology
- Dermatological diseases and infestations
- Immunodeficiency and Autoimmune Disorders
- Renal Transplantation Outcomes and Treatments
- Entomological Studies and Ecology
- Pediatric health and respiratory diseases
- Sympathectomy and Hyperhidrosis Treatments
- Study of Mite Species
- Lymphatic System and Diseases
- Contact Dermatitis and Allergies
- Reproductive System and Pregnancy
- Dermatological and Skeletal Disorders
- Potassium and Related Disorders
Linköping University
2013-2025
Linköping University Hospital
1996-2014
Sapienza University of Rome
2012
Hospital Clínico San Carlos
2010
Vall d'Hebron Hospital Universitari
2006-2010
Pediatrics and Genetics
2009
National University of Singapore
1996
Centro Nacional de Biopreparados (Cuba)
1995
The 65-kD isoform of glutamic acid decarboxylase (GAD) is a major autoantigen in patients with type 1 diabetes mellitus. This trial assessed the ability alum-formulated GAD (GAD-alum) to reverse recent-onset 10 18 years age.We randomly assigned 70 who had fasting C-peptide levels above 0.1 nmol per liter (0.3 ng milliliter) and autoantibodies, recruited within months after receiving diagnosis diabetes, receive subcutaneous injections 20 microg GAD-alum (35 patients) or placebo (alum alone,...
The 65-kD isoform of glutamic acid decarboxylase (GAD65) is a major autoantigen in type 1 diabetes. We hypothesized that alum-formulated GAD65 (GAD-alum) can preserve beta-cell function patients with recent-onset diabetes.We studied 334 patients, 10 to 20 years age, diabetes, fasting C-peptide levels more than 0.3 ng per milliliter (0.1 nmol liter), and detectable serum autoantibodies. Within 3 months after diagnosis, were randomly assigned receive one three study treatments: four doses...
CD4(+)CD25(high) regulatory T cells (Tregs) are implicated in the maintenance of murine pregnancy. However, reports regarding circulating Treg frequencies human pregnancy inconsistent, and functionality phenotype these have not been clarified. The aim this study was to determine frequency, phenotype, function Tregs second trimester influence progesterone 17beta-estradiol on frequency. Based expressions Foxp3, CD127, HLA-DR as determined by multicolor flow cytometry, we defined a proper...
The possible protective effect of breast milk against atopic manifestations in infancy, i.e. eczema and food allergy, has been controversial for the last decades. Besides methodological problems, differences composition human could explain these controversies. aim this study was to investigate polyunsaturated fatty acids (PUFA) secretory immunoglobulin A (S-IgA) levels proteins (ovalbumin beta-lactoglobulin) an inhalant allergen (cat) from mothers allergic non-allergic children. Blood...
OBJECTIVE To evaluate the efficacy of aluminum-formulated intralymphatic glutamic acid decarboxylase (GAD-alum) therapy combined with vitamin D supplementation in preserving endogenous insulin secretion all patients type 1 diabetes (T1D) or a genetically prespecified subgroup. RESEARCH DESIGN AND METHODS In multicenter, randomized, placebo-controlled, double-blind trial, 109 aged 12–24 years (mean ± SD 16.4 4.1) duration 7–193 days (88.8 51.4), elevated serum GAD65 autoantibodies, and...
Antigen-specific immunotherapy is an immunomodulatory strategy for autoimmune diseases, such as type 1 diabetes, in which patients are treated with autoantigens to promote immune tolerance, stop β-cell destruction and prevent permanent dependence on exogenous insulin. In this study, human proinsulin peptide C19-A3 (known its positive safety profile) was conjugated ultrasmall gold nanoparticles (GNPs), attractive drug delivery platform due the potential anti-inflammatory properties of gold....
OBJECTIVE Mixed-meal tolerance test (MMTT) area under the curve C-peptide (AUC CP) is gold-standard measure of endogenous insulin secretion in type 1 diabetes but intensive and invasive to perform. The 90-min MMTT-stimulated CP ≥0.2 nmol/L (90CP) related improved clinical outcomes, ≥0.1 equivalent fasting (FCP). We assessed whether 90CP or FCP are alternatives a full MMTT. RESEARCH DESIGN AND METHODS was measured during 1,334 MMTTs 421 patients aged <18 years at 3, 9, 18, 48, 72...
Micro RNAs (miRNAs) are promising disease biomarkers due to their high stability. Their expression in serum is altered type 1 diabetes, but whether deviations exist individuals with risk for diabetes remains unexplored. We therefore assessed miRNAs high-risk (n = 21) positive multiple islet autoantibodies, age-matched healthy children 17) and recent-onset patients 8), using Serum/Plasma Focus microRNA PCR Panels from Exiqon. The miRNA levels the group were similar controls, no specific...
Type 1 diabetes (T1D) is characterized by autoimmune destruction of insulin producing β-cells. The time from onset islet autoimmunity to manifest clinical disease can vary widely in length, and it fairly uncharacterized both clinically immunologically. In the current study, peripheral blood mononuclear cells autoantibody-positive children with high risk for T1D, age-matched healthy individuals, were analyzed mass cytometry using a panel 32 antibodies. Surface markers chosen identify multiple...
Abstract Aims Residual beta cell function in type 1 diabetes (T1D) is associated with lower risk of complications. Autoantigen therapy GAD-alum (Diamyd) given 3 intralymphatic injections oral vitamin D has shown promising results persons T1D carrying the human leukocyte antigen (HLA) DR3-DQ2 haplotype phase 2b trial DIAGNODE-2. We aimed to explore efficacy on blood glucose recorded by continuous monitoring (CGM). Methods DIAGNODE-2 (NCT03345004) was a multicenter, randomized,...
Abstract Background This study aimed to analyse data from two different studies (phase II and phase III) regarding the safety efficacy of treatment with alum formulated glutamic acid decarboxylase GAD 65 (GAD‐alum) at 30 months after administration children adolescents type 1 diabetes. Methods The trial was a double‐blind, randomised placebo‐controlled study, including 70 who were followed for months. Participants received subcutaneous injection either 20 µg GAD‐alum or placebo baseline...
Multiple islet autoimmunity increases risk of diabetes, but not all individuals positive for two or more autoantibodies progress to disease within a decade. Major recognise insulin (IAA), GAD (GADA), antigen-2 (IA-2A) and zinc transporter 8 (ZnT8A). Here we describe the baseline characteristics unique cohort 'slow progressors' (n = 132) who were multiple (IAA, GADA, IA-2A ZnT8A) did diabetes 10 years. Individuals identified from five studies (BABYDIAB, Germany; Diabetes Autoimmunity Study in...
In spite of intensive treatment Type 1 diabetes leads to serious complications. Preservation residual beta cell function makes the disease milder, facilitates treatment, prevents complications and increase survival. So far immune interventions have had limited effect, some adverse events risks. an open pilot trial we aimed improve efficacy GAD-alum using lymph-node administration in combination with oral vitamin D. Here report clinical effect focus on biomarkers for response treatment....
It is an established fact that T‐cell responses of fetal origin to inhalant allergens are present in most cord blood samples. These immune could be explained by trans‐placental passage peptides, either as free antigens or complexes with immunoglobulin G (IgG), providing the fetus a trigger for priming system already utero . The aim this study was investigate presence major cat allergen, Fel d 1, IgG and maternal sera. Serum samples from 75 mothers (38 allergic, 37 non‐allergic), their...
A phase II clinical trial with glutamic acid decarboxylase (GAD) 65 formulated aluminium hydroxide (GAD-alum) has shown efficacy in preserving residual insulin secretion children and adolescents recent-onset type 1 diabetes (T1D). We have performed a 4-year follow-up study of 59 the original 70 patients to investigate long-term cellular humoral immune responses after GAD-alum-treatment. Peripheral blood mononuclear cells (PBMC) were stimulated vitro GAD65. Frequencies naïve, central effector...
Islet amyloid polypeptide (IAPP) is a beta cell hormone secreted together with insulin upon glucose stimulation. IAPP participates in normal regulation, but also known for its ability to misfold and form islet amyloid. Amyloid fibrils through smaller toxic intermediates deposited disrupts architecture. Even though formation are much discussed type 2 diabetes, our aim was study the significance of 1 diabetes.Plasma levels children adolescents newly diagnosed diabetes (n = 224) were analysed...
Immunotherapies aimed at preserving residual beta cell function in type 1 diabetes have been successful, although the effect has limited, or raised safety concerns. Transient effects often observed may necessitate redosing to prolong effect, this is not always feasible safe. Treatment with intralymphatic GAD-alum shown be tolerable and safe persons significant efficacy preserve C-peptide associated clinical benefit individuals human leukocyte antigen DR3DQ2 haplotype. To further explore...
Mite species adapted to warm, humid climates are commonly found in house dust the tropics. In Cuba, Dermatophagoides pteronyssinus, D. siboney , and Blomiu tropicalis most common abundant mite dust. To investigate pattern of sensitization Cuban asthmatic patients species, we skin‐prick‐tested (SPT) 148 with a clinical history asthma possible allergy, determined specific IgE antibodies against allergens ( farinae, siboney, B. tropicalis, Acarus siro, Lepidoglyphus destructor, Tyrophagus...
OBJECTIVE GAD formulated in aluminum hydroxide (GAD-alum) has previously been shown to induce preservation of residual insulin secretion recent-onset type 1 diabetes, but recent phase II and III GAD-alum trials failed reach primary outcomes. The European study was therefore closed after 15 months, only a minority patients completed the 30 months follow-up. RESEARCH DESIGN AND METHODS This aimed characterize cellular humoral responses Swedish (n = 148) participating trial, receiving four (4D)...