Micro RNAs (miRNAs) are promising disease biomarkers due to their high stability. Their expression in serum is altered type 1 diabetes, but whether deviations exist individuals with risk for diabetes remains unexplored. We therefore assessed miRNAs high-risk (n = 21) positive multiple islet autoantibodies, age-matched healthy children 17) and recent-onset patients 8), using Serum/Plasma Focus microRNA PCR Panels from Exiqon. The miRNA levels the group were similar controls, no specific...

Toll-like receptor 2 (TLR2), which recognise and respond to conserved microbial pathogen-associated molecular patterns, is expressed on the platelet surface. Furthermore, it has recently been shown that TLR2/1 agonist Pam3CSK4 stimulates activation. The aim of present study was clarify important signalling events in Pam3CSK4-induced aggregation secretion. Platelet interaction with TLR2/6 MALP-2 studied by analysing aggregation, ATP-secretion, [Ca2+]i mobilisation thromboxane B2 (TxB2)...

Abstract. Objective. Several studies support an association between periodontal disease and atherosclerosis with a crucial role for the pathogen Porphyromonas gingivalis . This study aims at investigating proteolytic oxidative activity of P. on LDL in whole blood system using proteomic approach analysing effects ‐modified cell proliferation. Methods. The cellular human were assessed lumi‐aggregometry reactive oxygen species production aggregation. Blood was incubated 30 min gingivalis,...

Background: Enzymes of tricarboxylic acid (TCA) have recently been recognized as tumor suppressors. Mutations in the SDHB subunit succinate dehydrogenase (SDH) cause pheochromocytomas and paragangliomas (PCCs/PGLs) predispose patients to malignant disease with poor prognosis. Methods: Using human pheochromocytoma cell line (hPheo1), we knocked down gene expression using CRISPR-cas9 technology. Results: Microarray analysis showed that >500 differentially expressed targets, about 54%, were...

NLRP3 inflammasome is considered a critical actor in the inflammatory process of coronary artery disease. Increased expression components has been reported patients with acute syndrome, but whether this persists beyond phase less known. We performed prospective study to investigate basal and/or exercise-induced were elevated chronic syndrome (CCS). Patients (n = 81) underwent exercise stress tests twice: 3-4 weeks and 3-6 months after major event, whereas controls 30) it once. Concentrations...

Mitochondria play crucial roles in cellular metabolism, signaling, longevity and immune defense. Recent evidences have revealed that the host microbiota, including bacterial pathogens, impact mitochondrial behaviors activities host. The pathogenicity of Pseudomonas aeruginosa requires quorum sensing (QS) cell-cell communication allowing bacteria to sense population density collectively control biofilm development, virulence traits, adaptation interactions with QS molecules, like...

Fatty degeneration of thymus (or involution) has long been considered a normal ageing process. However, there is emerging evidence that thymic involution linked to T cell aging, chronic inflammation and increased morbidity. Other factors, aside from chronological age, have proposed affect the rate. In present study, we investigated imaging characteristics on computed tomography (CT) in Swedish middle-aged population. The major aims were establish prevalence fatty determine its associations...

Neutrophil extracellular traps (NETs) and mitochondrial DNA (mtDNA) are inflammatory mediators involved in the development of type 1 diabetes (T1D). Pancreas-infiltrating neutrophils can release NETs, contributing to process. Levels NETs increased serum from patients with T1D mtDNA is adult patients. Our aim was investigate (NETs, nuclear DNA) children newly diagnosed at high risk disease. We also elucidated if short after diagnosis could predict loss endogenous insulin production. Samples...

Hepatocyte growth factor (HGF) is an angiogenic, cardioprotective important for tissue and vascular repair. High levels of HGF are associated with chronic inflammatory diseases, such as coronary artery disease (CAD) periodontitis, suggested a marker the ongoing atherosclerotic event in patients CAD. Periodontal more prevalent among CAD than healthy people. Recent studies indicate reduced biological activity different conditions. Biologically active has high affinity to heparan sulfate...

A myelopoiesis gene signature in circulating leucocytes, exemplified by increased myeloperoxidase (MPO) and proteinase 3 (PR3) mRNA levels, has been reported patients with active anti-neutrophil cytoplasm antibody-associated vasculitis (AAV), to a lesser extent during remission. We hypothesized that this could predict disease relapse. levels of PR3, MPO, selected transcription factors [CCAAT/enhancer binding protein α (CEBP-α), CCAAT/enhancer β (CEBP-β), SPI1/PU.1-related factor (SPIB),...

The effect of daily treatments with 70% ethanol and 0.5% chlorhexidine in ethanol, respectively, on navel colonization newborns has been studied 624 infants two maternity wards during a four-month period. Staphylococcus aureus, group B G streptococci, E. coli anaerobes were significantly less often isolated from whose umbilical cord was treated than those only. epidermidis enterococci, the other hand, more chlorhexidine-ethanol group. More without umbilicus their fourth day life found...

We have previously shown that two injections of glutamic acid decarboxylase formulated in alum (GAD-alum) preserved residual insulin secretion children and adolescents with recent onset type 1 diabetes (T1D), was accompanied by increased GAD autoantibody (GADA) titers. The aim this study to investigate whether GAD-alum treatment affected the GADA epitope pattern.Serum samples from patients treated (n = 33) or placebo 27), at baseline, 1, 3, 9, 15 months after initial injection, were tested...

Abstract Background Fatty degeneration of thymus (or involution) has long been considered a normal ageing process and the role in adults drawn little attention. However, there is emerging evidence that thymic involution linked to T cell aging, chronic inflammation increased morbidity. Other factors than chronological age have proposed affect rate. its determinants studied at general population level. In present study, we investigated imaging characteristics on computed tomography (CT)...

Abstract Funding Acknowledgements Type of funding sources: Foundation. Main source(s): Heart and lung foundation Sweden. Background Over the last decades, significant improvements have been made in medical lifestyle managements patients with coronary artery disease (CAD). Nonetheless, "residual inflammatory risk" for recurrent cardiovascular events is high this patient group remains a diagnostic therapeutic challenge. Activation NLRP3 inflammasome, involving release interleukin (IL)-1β IL-18...

Abstract Helminth/tuberculosis (TB)-coinfection can reduce cell-mediated immunity against Mycobacterium tuberculosis (Mtb) and increase disease severity, although the effects are highly helminth species dependent. Mtb have long been ranked as number one single infectious agent claiming most lives. The only licensed vaccine for TB (BCG) offers variable protection TB, almost no transmission of Mtb. In recent few years identification naturally occurring antibodies in humans that protective...

Many patients with coronary artery disease (CAD) suffer from low-grade chronic inflammation indicating an increased risk of recurrent cardiac events. The inflammatory state in CAD has been associated elevated neutrophil counts, but also a deficiency regulatory T-cells (T regs ). role T the regulation survival proposed recently. Here, we hypothesised that interaction between and neutrophils might be altered patients, specifically investigated impact on apoptosis. Blood was collected 20 (mean...