A5045765874- Immune Cell Function and Interaction
- Hematopoietic Stem Cell Transplantation
- Acute Myeloid Leukemia Research
- T-cell and B-cell Immunology
- Chronic Lymphocytic Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Cancer, Hypoxia, and Metabolism
- PI3K/AKT/mTOR signaling in cancer
- Cancer-related Molecular Pathways
- CAR-T cell therapy research
- Cell death mechanisms and regulation
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- FOXO transcription factor regulation
- Neonatal Respiratory Health Research
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- HER2/EGFR in Cancer Research
- Immunodeficiency and Autoimmune Disorders
- Cancer-related gene regulation
- PARP inhibition in cancer therapy
- Cytokine Signaling Pathways and Interactions
- Advanced biosensing and bioanalysis techniques
Linköping University
2011-2023
Lund University
1999-2021
Scania (Sweden)
2021
University of Gothenburg
2012
Oslo University Hospital
2012
Institute of Cell Biology
2009
Uppsala University
2005
Ludwig Cancer Research
2005
Karolinska Institutet
2005
Malmö University
2000-2001
We have previously shown that mice carrying a null mutation in the homeobox gene ipf1, now renamed to pdx1, selectively lack pancreas. To elucidate level at which PDX1 is required during development of pancreas, we this study analyzed early stages pancreas ontogeny PDX-/- mice. These analyses revealed inductive events leading formation pancreatic buds and appearance insulin glucagon cells occur PDX1-deficient embryos. However, subsequent morphogenesis epithelium progression differentiation...
Extracellular superoxide dismutase (EC-SOD; superoxide:superoxide oxidoreductase, EC 1.15.1.1) is a secreted Cu- and Zn-containing tetrameric glycoprotein, the bulk of which bound to heparan sulfate proteoglycans in interstitium tissues. To test function EC-SOD vivo, mice carrying targeted disruption gene were generated. The null mutant develop normally remain healthy until at least 14 months age. No compensatory induction other SOD isoenzymes or antioxidant enzymes was observed. When...
CD4(+)CD25(high) regulatory T cells (Tregs) are implicated in the maintenance of murine pregnancy. However, reports regarding circulating Treg frequencies human pregnancy inconsistent, and functionality phenotype these have not been clarified. The aim this study was to determine frequency, phenotype, function Tregs second trimester influence progesterone 17beta-estradiol on frequency. Based expressions Foxp3, CD127, HLA-DR as determined by multicolor flow cytometry, we defined a proper...
Background The Q705K polymorphism in NLRP3 has been implicated several chronic inflammatory diseases. In this study we determine the functional role of commonly occurring using an in-vitro system. Principal Findings NLRP3-WT and NLRP3-Q705K were retrovirally transduced into human monocytic cell line THP-1, followed by assessment IL-1β IL-18 levels culture supernatant. THP-1 cells expressing above variants sorted based upon Green Fluorescent Protein (GFP) expression. Cytokine response to alum...
Immune-induced prostaglandin E2 (PGE2) synthesis is critical for fever and other centrally elicited disease symptoms. The production of PGE2 depends on cyclooxygenase-2 microsomal E synthase-1 (mPGES-1), but the identity cells involved has been a matter controversy. We generated mice expressing mPGES-1 either in hematopoietic or nonhematopoietic origin. Mice lacking displayed an intact febrile response to lipopolysaccharide, associated with elevated levels cerebrospinal fluid. In contrast,...
Abstract We studied the activation of small resting mouse T lymphocytes by antibodies to cell antigen receptor in combination with other surface antigens. Solid‐phase but not soluble KJ16‐133 and F23.1, both directed β chains V 8 family, activate cells proliferate presence growth factors, a dose‐dependent fashion. Antibodies Lyt‐2 L3T4 had no activating effect at any concentration. However, submitogenic concentrations F23.1 synergized wide range anti‐Lyt‐2 anti‐L3T4 cause proliferation...
Objective The tyrosine kinase inhibitors (TKIs) used in the treatment of chronic myeloid leukemia are substrates for efflux transport protein ATP-binding cassette subfamily G member 2 (ABCG2). Variations ABCG2 activity might influence pharmacokinetics and therapeutic outcome TKIs. role single-nucleotide polymorphisms (SNPs) TKI is not clear functional in-vitro studies lacking. aim this study was to investigate consequences SNPs efficacy TKIs [imatinib, N-desmethyl imatinib (CGP74588),...
Summary The proteinase‐activated receptor 1 ( PAR ‐1) plays a central role in melanoma progression and its expression level is believed to correlate with the degree of cancer invasiveness. Here, we show that ‐1 post‐transcriptionally regulated by miR‐20b micro RNA human cells. was found be expressed metastatic cells but barely detectable primary melanoma. By transducing lentivirus containing 3′‐ UTR construct mRNA , could endogenous s interacted silenced fused luciferase reporter....
Despite advances in the head and neck squamous cell carcinoma (HNSCC) treatment modalities, drug resistance cancer recurrence are often reported. Hypoxia signaling through hypoxia-inducible factor 1 (HIF-1) promotes angiogenesis metastasis by inducing epithelial-mesenchymal-transition (EMT). The aim of this study was to evaluate impact hypoxia on response therapy as well EMT expression stem markers HNSCC cells. Five lines (UT-SCC-2, UT-SCC-14, LK0412, LK0827, LK0923) were selected for study....
Hematopoietic development is a delicate balance of cell fate decisions in multipotent cells between self-renewal and differentiation. In multiple developmental systems, the Notch receptors areimportant factors regulating these processes. progenitor have been shown to express Notch1, studies with an activated intracellular form has revealed functional role. To assess function other members hematopoiesis, we investigated expression pattern Notch2, Notch3 hematopoietic lineages at level RNA...
Accumulating evidence suggests that hypoxic areas in the bone marrow are crucial for maintenance of hematopoietic stem cells (HSCs) by supporting a quiescent state cell cycle and regulating transplantation capacity long-term (LT)-HSCs. In addition, HSCs seem to express metabolic profile energy production away from mitochondrial oxidative phosphorylation favor glycolysis. At oxygen deprivation, hypoxia inducible factor 1α (HIF-1α) is known induce glycolytic enzymes as well suppressing...