A5013443180- Preterm Birth and Chorioamnionitis
- Reproductive System and Pregnancy
- Pregnancy and preeclampsia studies
- Pregnancy-related medical research
- Asthma and respiratory diseases
- IL-33, ST2, and ILC Pathways
- Neonatal Respiratory Health Research
- Eosinophilic Esophagitis
- Pregnancy and Medication Impact
- COVID-19 Impact on Reproduction
- Allergic Rhinitis and Sensitization
- Birth, Development, and Health
- Dermatology and Skin Diseases
- Contact Dermatitis and Allergies
- Reproductive tract infections research
- Food Allergy and Anaphylaxis Research
- Immune Cell Function and Interaction
- Inflammasome and immune disorders
- Urological Disorders and Treatments
- Infant Nutrition and Health
- Maternal and fetal healthcare
- Immune Response and Inflammation
- Cancer-related molecular mechanisms research
- Blood Coagulation and Thrombosis Mechanisms
- Gestational Diabetes Research and Management
National Center For Child Health and Development
2016-2025
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2018-2023
National Institutes of Health
2018-2023
Wayne State University
2018-2023
Hitachi (Japan)
2023
Center for Children
2023
United States Department of Health and Human Services
2020-2022
Health and Human Development (2HD) Research Network
2022
Pregnant women represent a high-risk population for severe/critical COVID-19 and mortality. However, the maternal-fetal immune responses initiated by SARS-CoV-2 infection, whether this virus is detectable in placenta, are still under investigation. Here we show that infection during pregnancy primarily induces unique inflammatory at interface, which largely governed maternal T cells fetal stromal cells. also associated with humoral cellular blood, as well mild cytokine response neonatal...
Cytokines employ downstream Janus kinases (JAKs) to promote chronic inflammatory diseases. JAK1-dependent type 2 cytokines drive allergic inflammation, and patients with JAK1 gain-of-function (GoF) variants develop atopic dermatitis (AD) asthma. To explore tissue-specific functions, we inserted a human GoF variant (JAK1GoF) into mice observed the development of spontaneous AD-like skin disease but unexpected resistance lung inflammation when JAK1GoF expression was restricted stroma. We...
Abstract Caesarean section (CS) is increasing globally, and women with prior CS are at higher risk of uterine rupture in subsequent pregnancies. However, little known about the incidence, factors, outcomes CS, especially developing countries. To investigate this, we conducted a secondary analysis World Health Organization Multicountry Survey on Maternal Newborn Health, which included data delivery from 359 facilities 29 The incidence among least one was 0.5% (170/37,366), ranging 0.2%...
Pregnancy is a stress factor culminating into mild endoplasmic reticulum (ER) stress, which necessary for placental development. However, excessive or chronic ER in pre-eclamptic placentas leads to dysfunction. The precise mechanisms through impacts trophoblasts are not well understood. Here, we showed that reduces the number of lysosomes, resulting inhibition autophagic flux trophoblast cells. also disrupted translocation lysosomes surface cells, and inhibited lysosomal exocytosis, whereby...
Parturition is a well-orchestrated process characterized by increased uterine contractility, cervical ripening, and activation of the chorioamniotic membranes; yet, transition from quiescent to contractile myometrium heralds onset labor. However, cellular underpinnings human parturition in tissues are still poorly understood. Herein, we performed comprehensive study during spontaneous term labor using single-cell RNA sequencing (scRNA-Seq). First, established atlas unraveled cell...
Epidemiological studies demonstrated that cleaning work and frequent use of products are risk factors for asthma. Laundry detergents have been reported to epithelial barrier-opening effects. However, whether laundry directly induce airway inflammation its mechanisms in vivo remain be elucidated.Two commercial two commonly used surfactants cosmetics (sodium lauryl sulfate sodium dodecyl benzene sulfonate) were intranasally administered mice. Lungs analyzed using flow cytometry, histology,...
Intra-amniotic infection is strongly associated with adverse pregnancy and neonatal outcomes. Most intra-amniotic infections are due to Ureaplasma species; however, the pathogenic potency of these genital mycoplasmas induce preterm birth still controversial. Here, we first laid out a taxonomic characterization isolates from women infection, which revealed that parvum most common bacterium found in this clinical condition. Next, using animal models, provided causal link between inoculation...
The prevailing paradigm in obstetrics has been the sterile womb hypothesis, which posits that fetuses are first colonized by microorganisms during delivery process. However, some now suggesting consistently utero from microbial communities inhabit placenta and intra-amniotic environment. Given established causal role of invasion amniotic cavity (i.e., infection) pregnancy complications, especially preterm birth, if colonization hypothesis were true, there several aspects current...
Preterm birth is the leading cause of neonatal morbidity and mortality worldwide. One every 4 preterm neonates born to a mother with intra-amniotic inflammation driven by invading bacteria. However, molecular mechanisms underlying this hostile immune response remain unclear. Here, we used translationally relevant model in Nlrp3-deficient -sufficient pregnant mice identify what believe previously unknown dual role for NLRP3 pathway fetal maternal signaling required premature onset labor...
Abstract Problem Preterm birth is commonly preceded by preterm labor, a syndrome that causally linked to both intra‐amniotic infection and inflammation. However, the stereotypical cellular immune responses in these two clinical conditions are poorly understood. Method of study Amniotic fluid samples (n = 26) were collected from women diagnosed with labor (amniotic IL‐6 concentrations ≥2.6 ng/mL culturable microorganisms, n 10) or inflammation without 16). Flow cytometry was performed...
Sterile intra-amniotic inflammation is a clinical condition frequently observed in women with preterm labor and birth, the leading cause of neonatal morbidity mortality worldwide. Growing evidence suggests that alarmins found amniotic fluid, such as interleukin (IL)-1α, are central initiators sterile inflammation. However, causal link between elevated concentrations IL-1α birth has yet to be established. Herein, using an animal model ultrasound-guided injection IL-1α, we show mortality....
Abstract Background Preterm birth is the leading cause of perinatal morbidity and mortality. prelabor rupture membranes (pPROM) occurs in 30% preterm births; thus, this complication a major contributor to maternal neonatal morbidity. However, cellular immune responses amniotic fluid women with pPROM have not been investigated. Methods Amniotic samples were obtained from positive (n = 7) or negative 10) microbiological culture. Flow cytometry was performed evaluate phenotype number...
Abstract Background The inflammasome has been implicated in the mechanisms that lead to spontaneous labor at term. However, whether is activated amniotic cavity of women with clinical chorioamnionitis term unknown. Herein, by measuring extracellular ASC [apoptosis-associated speck-like protein containing a C-terminal caspase recruitment domain (CARD)], we investigated there vivo activation fluid patients sterile intra-amniotic inflammation and those infection. Methods This was retrospective...
Background Intra-amniotic inflammation, which is associated with adverse pregnancy outcomes, can occur in the presence or absence of detectable microorganisms, and involves activation inflammasome. inflammasome has been reported clinical chorioamnionitis at term preterm labor intact membranes, but it not yet investigated women prelabor rupture membranes (preterm PROM) presence/absence microorganisms. The aim this study was to determine whether, among PROM, there an association between...
Preterm labor precedes premature birth, the leading cause of neonatal morbidity and mortality worldwide. can occur in context either microbe-associated intra-amniotic inflammation (i.e., infection) or absence detectable microorganisms sterile inflammation). Both infection trigger local immune responses that have deleterious effects on fetal life. Yet, extent such tissues surrounding amniotic cavity chorioamniotic membranes) is poorly understood. By using RNA sequencing (RNA seq) as a...
Background IL-25, IL-33 and TSLP are produced predominantly by epithelial cells known to induce Th2-type cytokines. cytokines involved not only in host defense against nematodes, but also the development of allergic diseases. was reported be crucial for airway inflammation mice after inhalation allergens which they had been sensitized epicutaneously (EC) beforehand. However, roles IL-25 setting remain unclear. Methods Mice deficient were EC with ovalbumin (OVA) then challenged intranasally...