A5066102879- Preterm Birth and Chorioamnionitis
- Neonatal Respiratory Health Research
- Reproductive System and Pregnancy
- Pregnancy and preeclampsia studies
- Neuroscience of respiration and sleep
- Pregnancy-related medical research
- Neonatal and fetal brain pathology
- Infant Nutrition and Health
- Neuroendocrine regulation and behavior
- Birth, Development, and Health
- Infant Development and Preterm Care
- Photoreceptor and optogenetics research
- Sleep and Wakefulness Research
- Neuroscience and Neuropharmacology Research
- IL-33, ST2, and ILC Pathways
- Inflammasome and immune disorders
- Reproductive tract infections research
- Neonatal and Maternal Infections
- Gestational Diabetes Research and Management
- Intestinal and Peritoneal Adhesions
- COVID-19 Impact on Reproduction
- Congenital Diaphragmatic Hernia Studies
- Antimicrobial Peptides and Activities
- Maternal and fetal healthcare
- Neonatal Health and Biochemistry
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2016-2025
Research Network (United States)
2021-2025
National Institutes of Health
2017-2023
Wayne State University
2015-2023
Eunice Kennedy Shriver Center
2017-2023
United States Department of Health and Human Services
2016-2023
Health and Human Development (2HD) Research Network
2022-2023
Detroit R&D (United States)
2022
Michigan United
2017-2019
Children's Hospital of Michigan
2017-2019
Background Microbial invasion of the amniotic cavity resulting in intra-amniotic infection is associated with obstetrical complications such as preterm labor intact or ruptured membranes, cervical insufficiency, well clinical and histological chorioamnionitis. The most widely accepted pathway for ascension microorganisms from lower genital tract. However, hematogenous dissemination oral intestine, retrograde seeding peritoneal through fallopian tubes, introduction invasive medical procedures...
Objective To determine the frequency and type of histopathologic lesions in placentas delivered by women with a normal pregnancy outcome. Methods This retrospective cohort study included placental samples from 944 singleton gestation who at term without obstetrical complications. Placental were classified into following four categories as defined Society for Pediatric Pathology our unit: (1) acute inflammation, (2) chronic (3) maternal vascular malperfusion (4) fetal malperfusion. Results...
Problem Sterile intra‐amniotic inflammation is associated with spontaneous preterm labor. Alarmins are proposed to mediate this inflammatory process. The aim of study was determine whether administration an alarmin, HMGB 1, could induce labor/birth. Method Study Pregnant B6 mice were intra‐amniotically or intraperitoneally injected 1 PBS (control). Following injection, the gestational age and rates birth pup mortality recorded. Results Intra‐amniotic injection led labor/birth [ 57% (4/7)...
Preterm labor commonly precedes preterm birth, the leading cause of perinatal morbidity and mortality worldwide. Most research has focused on establishing a causal link between innate immune activation pathological inflammation to birth. However, role maternal effector/activated T cells in pathogenesis labor/birth is poorly understood. In this study, we first demonstrated that effector memory activated expressing granzyme B perforin are enriched at maternal-fetal interface (decidua) women...
To identify maternal plasma protein markers for early preeclampsia (delivery <34 weeks of gestation) and to determine whether the prediction performance is affected by disease severity presence placental lesions consistent with vascular malperfusion (MVM) among cases.
Background Late-onset preeclampsia is the most prevalent phenotype of this syndrome; nevertheless, only a few biomarkers for its early diagnosis have been reported. We sought to correct deficiency using high through-put proteomic platform. Methods A case-control longitudinal study was conducted, including 90 patients with normal pregnancies and 76 late-onset (diagnosed at ≥34 weeks gestation). Maternal plasma samples were collected throughout gestation (normal pregnancy: 2–6 per patient,...
Problem Pathological inflammation is causally linked to preterm labor and birth, the leading cause of neonatal morbidity mortality worldwide. Our aims were investigate whether (i) newly described family innate lymphoid cells (ILCs) was present at human maternal‐fetal interface (ii) ILC inflammatory subsets associated with pathological process labor. Methods study Decidual leukocytes isolated from women or term as well gestational age‐matched non‐labor controls. ILCs (CD15 − CD14 CD3 CD19...
Sterile intra-amniotic inflammation is commonly observed in patients with spontaneous preterm labor, a syndrome that precedes birth, the leading cause of perinatal morbidity and mortality worldwide. However, mechanisms to sterile are poorly understood no treatment exists for this clinical condition. Herein, we investigated whether alarmin S100B could induce by activating NLRP3 inflammasome, inhibition pathway prevent labor/birth adverse neonatal outcomes. We found ultrasound-guided...
Preterm birth, the leading cause of perinatal morbidity and mortality worldwide, frequently results from syndrome preterm labor. The best-established causal link to labor is intra-amniotic infection, which involves premature activation parturition cascade in reproductive tissues. Herein, we utilize single-cell RNA sequencing (scRNA-seq) generate a atlas murine uterus, decidua, cervix model infection-induced We show that affects transcriptomic profiles specific immune non-immune cell subsets....
The inflammasome is implicated in the mechanisms that lead to spontaneous preterm labor (PTL). However, whether there activation amniotic cavity of women with PTL and intra-amniotic infection (IAI) or sterile inflammation (SIAI) unknown.Amniotic fluid samples were collected from who delivered at term (n = 31) without IAI SIAI 35), 27), 17). As a readout activation, extracellular ASC (apoptosis-associated speck-like protein containing CARD) was measured by ELISA expression ASC, caspase-1,...
Problem The immune cellular composition of amniotic fluid is poorly understood. Herein, we determined: 1) the immunophenotype cells during second and third trimester in absence intra‐amniotic infection/inflammation; 2) whether T ILCs display different phenotypical characteristics to that peripheral cells; 3) are altered women with infection/inflammation. Method Study Amniotic samples (n = 57) were collected from 15 40 weeks gestation without Samples infection/inflammation also included 9)....
Abstract Preterm birth (PTB) is the leading cause of neonatal morbidity and mortality worldwide. Although intra-amniotic infection a recognized spontaneous preterm labor, noninfection-related etiologies are poorly understood. In this article, we demonstrated that expansion activated CD1d-restricted invariant NKT (iNKT) cells in third trimester by administration α-galactosylceramide (α-GalCer) induced late PTB mortality. vivo imaging revealed fetuses from mice underwent α-GalCer–induced had...
Problem Neutrophils are capable of performing phagocytosis, a primary mechanism for microbial killing. Intra‐amniotic infection is characterized by an influx neutrophils into the amniotic cavity. Herein, we investigated whether fluid could phagocytize bacteria found in cavity women with intra‐amniotic infection. Methods Amniotic from were visualized transmission electron microscopy (n=6). The phagocytic activity and/or inflammation (n=10) or peripheral healthy individuals (controls, n=3) was...
Preterm birth is the leading cause of neonatal morbidity and mortality worldwide. Inflammation causally linked to preterm birth; therefore, finding an intervention that dampens maternal fetal inflammatory responses may provide a new strategy prevent adverse pregnancy outcomes. Using animal models systemic inflammation (intraperitoneal injection lipopolysaccharide) (intra-amniotic administration lipopolysaccharide), we found that: 1) induced outcomes by causing severe cytokine storm mild...
Intra-amniotic infection is associated with spontaneous preterm labor. In most cases, the subclinical and bacteria are detected in amniotic cavity rather than chorioamniotic membranes. The aims of this study were to establish a model intra-amniotic lipopolysaccharide (LPS)-induced labor/birth that resembles syndrome compare two established models LPS-induced labor/birth.Pregnant B6 mice received an intra-amniotic, intra-uterine, or intra-peritoneal injection LPS (100 ng/amniotic sac, 15...
Abstract Problem Preterm birth is commonly preceded by preterm labor, a syndrome that causally linked to both intra‐amniotic infection and inflammation. However, the stereotypical cellular immune responses in these two clinical conditions are poorly understood. Method of study Amniotic fluid samples (n = 26) were collected from women diagnosed with labor (amniotic IL‐6 concentrations ≥2.6 ng/mL culturable microorganisms, n 10) or inflammation without 16). Flow cytometry was performed...
Human chorionic gonadotropin (hCG) is implicated in the maintenance of uterine quiescence by down-regulating myometrial gap junctions during pregnancy, and it was considered as a strategy to prevent preterm birth after occurrence labor. However, effect hCG on innate adaptive immune cells parturition poorly understood. Herein, we investigated effects at maternal-fetal interface late gestation, whether this hormone can safely endotoxin-induced birth. Using immunophenotyping, demonstrated that...