Michelle Wantoch
A5056767704- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- COVID-19 epidemiological studies
- SARS-CoV-2 detection and testing
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- COVID-19 and healthcare impacts
- Chronic Myeloid Leukemia Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- COVID-19 Pandemic Impacts
- Viral-associated cancers and disorders
- CAR-T cell therapy research
- Lymphoma Diagnosis and Treatment
- T-cell and B-cell Immunology
- Prostate Cancer Treatment and Research
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Immunology Research
- Acute Myeloid Leukemia Research
- Hematopoietic Stem Cell Transplantation
- CRISPR and Genetic Engineering
- Eosinophilic Disorders and Syndromes
Wellcome/MRC Cambridge Stem Cell Institute
2020-2024
University of Cambridge
2020-2024
Medical Research Council
2022
University of Leeds
2017-2022
National Institute for Health Research
2021
MRC Biostatistics Unit
2021
Public Health England
2021
UK Research and Innovation
2021
Cambridge School
2020
Stem Cell Institute
2020
Global dispersal and increasing frequency of the SARS-CoV-2 spike protein variant D614G are suggestive a selective advantage but may also be due to random founder effect. We investigate hypothesis for positive selection in United Kingdom using more than 25,000 whole genome sequences. Despite availability large dataset, well represented by both 614 variants, not all approaches showed conclusive signal selection. Population genetic analysis indicates that 614G increases relative 614D manner...
Significant differences exist in the availability of healthcare worker (HCW) SARS-CoV-2 testing between countries, and existing programmes focus on screening symptomatic rather than asymptomatic staff. Over a 3 week period (April 2020), 1032 HCWs were screened for large UK teaching hospital. Symptomatic staff household contacts additionally tested. Real-time RT-PCR was used to detect viral RNA from throat+nose self-swab. 3% group tested positive SARS-CoV-2. 17/30 (57%) truly...
The SARS-CoV-2 delta (B.1.617.2) variant was first detected in England March, 2021. It has since rapidly become the predominant lineage, owing to high transmissibility. is suspected that associated with more severe disease than previously dominant alpha (B.1.1.7) variant. We aimed characterise severity of compared by determining relative risk hospital attendance outcomes.
Previously, we showed that 3% (31/1032)of asymptomatic healthcare workers (HCWs) from a large teaching hospital in Cambridge, UK, tested positive for SARS-CoV-2 April 2020. About 15% (26/169) HCWs with symptoms of coronavirus disease 2019 (COVID-19) also (Rivett et al., 2020). Here, show the proportion both and symptomatic testing rapidly declined to near-zero between 25th 24th May 2020, corresponding decline patient admissions COVID-19 during ongoing UK 'lockdown'. These data demonstrate...
Adult hematopoietic stem cells (HSCs) are predominantly quiescent and can be activated in response to acute stress such as infection or cytotoxic insults. STAT1 is a pivotal downstream mediator of interferon (IFN) signaling required for IFN-induced HSC proliferation, but little known about the role regulating homeostatic stem/progenitor (HSPCs). Here, we show that loss altered steady state HSPC landscape, impaired function transplantation assays, delayed blood cell regeneration following...
Abstract Mitigation of SARS-CoV-2 transmission from international travel is a priority. We evaluated the effectiveness travellers being required to quarantine for 14-days on return England in Summer 2020. identified 4,207 travel-related cases and their contacts, 827 associated genomes. Overall, was with lower rate impact greatest 16–20 age-group. 186 genomes were sufficiently unique identify clusters. Fewer genomically-linked observed index who returned countries requirement compared no...
Prostate cancer research is hampered by the lack of in vivo preclinical models that accurately reflect patient tumour biology and clinical heterogeneity human prostate cancer. To overcome these limitations we propagated characterised a new collection patient-derived xenografts. Tumour fragments from 147 unsupervised, surgical samples were implanted subcutaneously into immunodeficient Rag2-/-γC-/- mice within 24 hours surgery. Histologic molecular characterisation xenografts was compared with...
Abstract Normal and malignant hematopoietic stem cells (HSCs) are controlled by extracellular cues including cytokine signalling through the JAK/STAT pathway. Here, we show that STAT5-deficient HSCs exhibit an unusual phenotype: while reduced multi-lineage repopulation self-renewal commonly associated with overproliferation exhaustion, they instead cell-cycle progression increased differentiation in HSCs. Mechanistic studies unphosphorylated-STAT5 (uSTAT5) contributes to this phenotype...
Natural killer (NK) cells protect against intracellular infection and cancer. These properties are exploited in oncolytic virus (OV) therapy, where antiviral responses enhance anti-tumour immunity. We have analysed the mechanism by which reovirus, an dsRNA virus, modulates human NK cell activity. Reovirus activates a type I interferon (IFN-I) dependent manner, inducing STAT1 STAT4 signalling both CD56
Abstract Natural killer (NK) cells protect against intracellular infection and cancer. These properties are exploited in oncolytic virus (OV) therapy, where anti-viral responses enhance anti-tumour immunity. We have analysed the mechanism by which reovirus, an dsRNA virus, modulates human NK cell activity. Reovirus activates a type I interferon (IFN-I) dependent manner, resulting STAT1 STAT4 signalling both CD56 dim bright subsets. Gene expression profiling revealed dominance of IFN-I...