Adrian W. Signell
A5010919637- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Biosensors and Analytical Detection
- COVID-19 epidemiological studies
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Respiratory viral infections research
- HIV Research and Treatment
- Peptidase Inhibition and Analysis
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- Cancer Immunotherapy and Biomarkers
- Infection Control and Ventilation
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- CAR-T cell therapy research
King's College London
2019-2022
University College London
2021
Guy's Hospital
2019
Antibody responses to SARS-CoV-2 can be detected in most infected individuals 10-15 d after the onset of COVID-19 symptoms. However, due recent emergence human population, it is not known how long antibody will maintained or whether they provide protection from reinfection. Using sequential serum samples collected up 94 post symptoms (POS) 65 with real-time quantitative PCR-confirmed infection, we show seroconversion (immunoglobulin (Ig)M, IgA, IgG) >95% cases and neutralizing when sampled...
The SARS-CoV-2 delta (B.1.617.2) variant was first detected in England March, 2021. It has since rapidly become the predominant lineage, owing to high transmissibility. is suspected that associated with more severe disease than previously dominant alpha (B.1.1.7) variant. We aimed characterise severity of compared by determining relative risk hospital attendance outcomes.
Abstract Antibody (Ab) responses to SARS-CoV-2 can be detected in most infected individuals 10-15 days following the onset of COVID-19 symptoms. However, due recent emergence this virus human population it is not yet known how long these Ab will maintained or whether they provide protection from re-infection. Using sequential serum samples collected up 94 post symptoms (POS) 65 RT-qPCR confirmed SARS-CoV-2-infected individuals, we show seroconversion >95% cases and neutralizing antibody...
There is a clear requirement for an accurate SARS-CoV-2 antibody test, both as complement to existing diagnostic capabilities and determining community seroprevalence. We therefore evaluated the performance of variety testing technologies their potential use tools. Highly specific in-house ELISAs were developed detection anti-spike (S), -receptor binding domain (RBD) -nucleocapsid (N) antibodies used cross-comparison ten commercial serological assays—a chemiluminescence-based platform, two...
There is a worldwide need for reagents to perform SARS-CoV-2 detection. Some laboratories have implemented kit-free protocols, but many others do not the capacity develop these and/or manual processing. We provide multiple workflows nucleic acid detection in clinical samples by comparing several commercially available RNA extraction methods: QIAamp Viral Mini Kit (QIAgen), RNAdvance Blood/Viral (Beckman) and Mag-Bind DNA/RNA 96 (Omega Bio-tek). also compared One-step RT-qPCR reagents: TaqMan...
An evaluation of a rapid portable gold-nanotechnology measuring SARS-CoV-2 IgM, IgA and IgG antibody response to spike 1 (S1), 2 (S) nucleocapsid (N) antigens using serum from 74 RNA(+) patients 47 control patients.
Abstract There is a clear requirement for an accurate SARS-CoV-2 antibody test, both as complement to existing diagnostic capabilities and determining community seroprevalence. We therefore evaluated the performance of variety testing technologies their potential tools. A highly specific in-house ELISA was developed detection anti-spike (S), -receptor binding domain (RBD) -nucleocapsid (N) antibodies used cross-comparison ten commercial serological assays – chemiluminescence-based platform,...
Abstract There is a worldwide need for reagents to perform SARS-CoV-2 detection. Some laboratories have implemented kit-free protocols, but many others do not the capacity develop these and/or manual processing. We provide multiple workflows nucleic acid detection in clinical samples by comparing several commercially available RNA extraction methods: QIAamp Viral Mini Kit (QIAgen), RNAdvance Blood/Viral (Beckman) and Mag-Bind DNA/RNA 96 (Omega Bio-tek). also compared One-step RT-qPCR...
During the first wave of global COVID-19 pandemic clinical utility and indications for SARS-CoV-2 serological testing were not clearly defined. The urgency to deploy assays required rapid evaluation their performance characteristics. We undertook an internal validation a CE marked lateral flow immunoassay (LFIA) (SureScreen Diagnostics) using serum from RNA positive individuals pre-pandemic samples. This was followed by delivery same-day named patient serology service LFIA on vetted...
ABSTRACT OBJECTIVE Determine indications and clinical utility of SARS-CoV-2 serology testing in adults children. DESIGN Prospective evaluation initial three weeks a daily Monday to Friday pilot service for patients. SETTING Early post “first-wave” transmission period at single centre London teaching hospital that provides care the local community, as well regional national referral pathways specialist services. PARTICIPANTS 110 (72 adults, 38 children, age range 0-83 years, 52.7% female...
ABSTRACT Many healthcare facilities report SARS-CoV-2 outbreaks but transmission analysis is complicated by the high prevalence of infection and limited viral genetic diversity. The contribution different vectors to nosocomial or effectiveness interventions therefore currently unclear. Detailed epidemiological nanopore sequence data were analysed from 574 consecutive patients with a PCR positive test between March 13th 31st, when pandemic first impacted on large, multisite institution in...
The antiviral protein ZAP binds CpG dinucleotides in viral RNA to inhibit replication. This has likely led the suppression observed many viruses, including retroviruses. Sequences added retroviral vector genomes, such as internal promoters, transgenes, or regulatory elements, substantially increase abundance. Because these CpGs could allow be targeted by ZAP, we analyzed whether it restricts production, transduction efficiency, and transgene expression. Surprisingly, even though CpG-high...