A5077214934- Hepatitis C virus research
- Hepatitis B Virus Studies
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- HIV Research and Treatment
- Liver Disease Diagnosis and Treatment
- SARS-CoV-2 detection and testing
- HIV/AIDS drug development and treatment
- Liver physiology and pathology
- Pancreatic function and diabetes
- Cancer, Hypoxia, and Metabolism
- Biochemical and Molecular Research
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Vectors
- Immune Cell Function and Interaction
- Evolution and Genetic Dynamics
- Viral Infections and Outbreaks Research
- interferon and immune responses
- Viral gastroenteritis research and epidemiology
- Computational Drug Discovery Methods
- Biosensors and Analytical Detection
- Mosquito-borne diseases and control
- Molecular Communication and Nanonetworks
- Wireless Body Area Networks
- COVID-19 diagnosis using AI
King's College London
2020-2024
Universidad de Málaga
2023-2024
Instituto de Investigación Biomédica de Málaga
2023-2024
Imperial College London
2016-2023
Centro de Biología Molecular Severo Ochoa
2013-2022
Universidad Autónoma de Madrid
2013-2022
Consejo Superior de Investigaciones Científicas
2013-2022
Abstract With more than 240 million people infected, hepatitis B virus (HBV) is a major health concern. The inability to mimic the complexity of liver using cell lines and regular primary human hepatocyte (PHH) cultures pose significant limitations for studying host/pathogen interactions. Here, we describe 3D microfluidic PHH system permissive HBV infection, which can be maintained at least 40 days. This enables recapitulation all steps life cycle, including replication patient-derived...
COVID-19 vaccine design and vaccination rollout need to take into account a detailed understanding of antibody durability cross-neutralizing potential against SARS-CoV-2 emerging variants concern (VOCs). Analyses convalescent sera provide unique insights longevity activity induced by variant spike proteins, which are putative candidates. Using from 38 individuals infected in wave 1, we show that can be detected up 305 days pos onset symptoms, although were less potent B.1.1.7 (Alpha) B1.351...
Viruses are a major threat to human health and economic well-being. In recent years Ebola, Zika, influenza, chikungunya virus epidemics have raised awareness that infections can spread rapidly before vaccines or specific antagonists be made available. Broad-spectrum antivirals drugs with the potential inhibit infection by viruses from different groups families, which may deployed during outbreaks when diagnostics, directly acting not While pathogen-directed approaches generally effective...
Lethal mutagenesis, or virus extinction produced by enhanced mutation rates, is under investigation as an antiviral strategy that aims at counteracting the adaptive capacity of viral quasispecies, and avoiding selection antiviral-escape mutants. To explore lethal mutagenesis hepatitis C (HCV), it important to establish whether ribavirin, purine nucleoside analogue used in anti-HCV therapy, acts a mutagenic agent during replication cell culture. Here we report effect ribavirin serial passages...
Zika virus (ZIKV) Infection has several outcomes from asymptomatic exposure to rash, conjunctivitis, Guillain-Barré syndrome or congenital syndrome. Analysis of ZIKV immunity is confounded by the fact that related Flaviviruses infect humans, including Dengue 1-4, West Nile and Yellow Fever virus. HLA class II restricted T cell cross-reactivity between other infection(s) vaccination may contribute protection enhanced immunopathology. We mapped immunodominant, restricted, CD4 epitopes Envelope...
There is a worldwide need for reagents to perform SARS-CoV-2 detection. Some laboratories have implemented kit-free protocols, but many others do not the capacity develop these and/or manual processing. We provide multiple workflows nucleic acid detection in clinical samples by comparing several commercially available RNA extraction methods: QIAamp Viral Mini Kit (QIAgen), RNAdvance Blood/Viral (Beckman) and Mag-Bind DNA/RNA 96 (Omega Bio-tek). also compared One-step RT-qPCR reagents: TaqMan...
Despite the exceptional infectivity of hepatitis B virus (HBV) in vivo, where only three viral genomes can result a chronicity experimentally infected chimpanzees, most vitro models require several hundreds to thousands per cell order initiate transient infection. Additionally, static 2D cultures primary human hepatocytes (PHH) allow short-term studies due their rapid dedifferentiation. Here, we describe 3D liver-on-a-chip PHH, either monocultures or cocultures with other nonparenchymal...
Abstract There is a worldwide need for reagents to perform SARS-CoV-2 detection. Some laboratories have implemented kit-free protocols, but many others do not the capacity develop these and/or manual processing. We provide multiple workflows nucleic acid detection in clinical samples by comparing several commercially available RNA extraction methods: QIAamp Viral Mini Kit (QIAgen), RNAdvance Blood/Viral (Beckman) and Mag-Bind DNA/RNA 96 (Omega Bio-tek). also compared One-step RT-qPCR...
HCV vaccine development is stymied by the high genetic diversity of virus and variability envelope glycoproteins. One strategy to overcome this identify conserved, functionally important regions-such as epitopes broadly neutralizing antibodies (bNAbs)-and use these a basis for structure-based design. Here, we report an anti-idiotype approach that has generated antibody mimics highly conserved epitope on E2. Crucially, mutagenesis screen was used antibody, designated B2.1 A, whose binding...
The COVID-19 pandemic has accelerated the need to identify new antiviral therapeutics at pace, including through drug repurposing. We employed a Quadratic Unbounded Binary Optimization (QUBO) model, search for compounds similar Remdesivir, first against SARS-CoV-2 approved human use, using quantum-inspired device. modelled Remdesivir and present in DrugBank database as graphs, established optimal parameters our algorithm resolved Maximum Weighted Independent Set problem within conflict graph...
High-density lipoprotein (HDL) levels are reduced in patients with coronavirus disease 2019 (COVID-19), and the extent of this reduction is associated poor clinical outcomes. While lipoproteins known to play a key role during life cycle hepatitis C virus, their influence on (CoV) infections poorly understood. In study, we utilize cross-linking mass spectrometry (XL-MS) determine circulating protein interactors severe acute respiratory syndrome (SARS)-CoV-2 spike glycoprotein. XL-MS plasma...
Hepatitis B virus (HBV) entry into hepatocytes is mediated via a high-affinity interaction between the preS1 glycoprotein and sodium/bile acid cotransporting polypeptide (NTCP). To date, in vitro model systems rely on high multiplicities of infection to achieve cell lines overexpressing human NTCP. This study investigates novel regulatory pathway for NTCP trafficking surface, induced by DMSO-mediated cellular differentiation. DMSO rapidly induces surface expression results increased...
As SARS-CoV-2 variants continue to emerge globally, a major challenge for COVID-19 vaccination is the generation of durable antibody response with cross-neutralizing activity against both current and newly emerging viral variants. Cross-neutralizing concern (B.1.1.7, P.1 B.1.351) has been observed following vaccination, albeit at reduced potency, but whether vaccines based on Spike glycoprotein these will produce superior not fully investigated. Here, we used sera from individuals infected...
Regenerative medicine aims to replace damaged tissues by stimulating endogenous tissue repair or transplanting autologous allogeneic cells. Due their capacity produce unlimited numbers of cells a given cell type, pluripotent stem cells, whether embryonic origin induced via the reprogramming somatic are considerable therapeutic interest in regenerative field. However, regardless host immune responses present barrier success. The aim this study was investigate vitro immunological properties...
In the course of experiments aimed at deciphering inhibition mechanism mycophenolic acid and ribavirin in hepatitis C virus (HCV) infection, we observed an inhibitory effect nucleoside guanosine (Gua). Here, report that Gua, not other standard nucleosides, inhibits HCV replication human hepatoma cells. Gua did directly inhibit vitro polymerase activity NS5B, but it modified intracellular levels di- tri-phosphates (NDPs NTPs), leading to deficient RNA reduction infectious progeny production....