A5040172012- Vibrio bacteria research studies
- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Immune Response and Inflammation
- Escherichia coli research studies
- Bacterial Genetics and Biotechnology
- Endoplasmic Reticulum Stress and Disease
- Biomedical Research and Pathophysiology
- RNA and protein synthesis mechanisms
- Gut microbiota and health
- Microtubule and mitosis dynamics
- Zebrafish Biomedical Research Applications
- Plant Pathogens and Fungal Diseases
- RNA regulation and disease
- Phagocytosis and Immune Regulation
- Antibiotic Resistance in Bacteria
- Viral gastroenteritis research and epidemiology
- Bacterial biofilms and quorum sensing
- Lipid Membrane Structure and Behavior
- Inflammasome and immune disorders
- Prion Diseases and Protein Misfolding
- Mass Spectrometry Techniques and Applications
- Aquaculture disease management and microbiota
University of Cambridge
2015-2024
MRC Laboratory of Molecular Biology
2024
Imperial College London
2016-2019
University of London
2018-2019
London School of Hygiene & Tropical Medicine
2018-2019
Healthcare Infection Society
2018
University of Bonn
2018
The major human bacterial pathogen
Septins, cytoskeletal proteins with well-characterised roles in cytokinesis, form cage-like structures around cytosolic Shigella flexneri and promote their targeting to autophagosomes. However, the processes underlying septin cage assembly, whether they influence S. proliferation, remain be established. Using single-cell analysis, we show that cages inhibit proliferation. To study mechanisms of used proteomics found mitochondrial associate septins flexneri-infected cells. Strikingly,...
The cytoskeleton occupies a central role in cellular immunity by promoting bacterial sensing and antibacterial functions. Septins are cytoskeletal proteins implicated various processes, including cell division. also assemble into cage-like structures that entrap cytosolic Shigella, yet how septins recognize bacteria is poorly understood. Here, we discover recruited to regions of micron-scale membrane curvature upon invasion division variety species. Cardiolipin, curvature-specific...
Professional phagocytic cells such as macrophages are a central part of innate immune defence. They ingest microorganisms into membrane-bound compartments (phagosomes), which acidify and eventually fuse with lysosomes, exposing their contents to microbicidal environment. Gram-positive Rhodococcus equi can cause pneumonia in young foals immunocompromised humans. The possession virulence plasmid allows them subvert host defence mechanisms multiply macrophages. Here, we show that the...
Shigella flexneri is historically regarded as the primary agent of bacillary dysentery, yet closely-related sonnei replacing S. flexneri, especially in developing countries. The underlying reasons for this dramatic shift are mostly unknown. Using a zebrafish (Danio rerio) model infection, we discover that more virulent than vivo. Whole animal dual-RNAseq and testing bacterial mutants suggest virulence depends on its O-antigen oligosaccharide (which unique among species). We show vivo using...
Shigella flexneri, a Gram-negative enteroinvasive pathogen, causes inflammatory destruction of the human intestinal epithelium. During infection epithelial cells, escape from phagosome to cytosol, where they reroute host cell glycolysis obtain nutrients for proliferation. Septins, poorly understood component cytoskeleton, can entrap cytosolic targeted autophagy in cage-like structures restrict bacterial Although entrapment by septin caging has been subject intense investigation, role septins...
ABSTRACT Pathogenic Shigella bacteria are a paradigm to address key issues of cell and infection biology. Polar localisation the autotransporter protein IcsA is essential for actin tail formation, which necessary bacterium travel from cell-to-cell; yet how proteins targeted bacterial pole poorly understood. The homologue MreB has been extensively studied in broth culture using model organisms including Escherichia coli, Bacillus subtilis Caulobacter crescentus, but never visualised...
Antimicrobial resistance is a major threat the world currently facing. Development of new antibiotics and assessment their toxicity represent important challenges. Current methods for addressing antibiotic rely on measuring mitochondrial damage using ATP and/or membrane potential as readout. In this study, we propose an alternative readout looking at changes in lipidome intact unprocessed cells by matrix-assisted laser desorption ionization mass spectrometry. As proof principle, evaluated...
Abstract Shigella flexneri is historically regarded as the primary agent of bacillary dysentery, yet closely-related sonnei replacing S. , especially in developing countries. The underlying reasons for this dramatic shift are mostly unknown. Using a zebrafish ( Danio rerio ) model infection, we discover that more virulent than vivo . Whole animal dual-RNAseq and testing bacterial mutants suggest virulence depends on its O-antigen oligosaccharide (which unique among species). We show using ex...