A5013548456- Zebrafish Biomedical Research Applications
- Infectious Disease Case Reports and Treatments
- Aquaculture disease management and microbiota
- Immune cells in cancer
- Infections and bacterial resistance
- Nanoparticle-Based Drug Delivery
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Lipid Membrane Structure and Behavior
- Biomedical Research and Pathophysiology
- Cancer therapeutics and mechanisms
- Advanced Fluorescence Microscopy Techniques
- Extracellular vesicles in disease
- Single-cell and spatial transcriptomics
- Phagocytosis and Immune Regulation
- Microbial Community Ecology and Physiology
- Enzyme Structure and Function
- Cancer Research and Treatments
- Phenothiazines and Benzothiazines Synthesis and Activities
- Data Visualization and Analytics
- Antimicrobial agents and applications
- Antimicrobial Peptides and Activities
- Marine and coastal plant biology
- Microbial Metabolism and Applications
University of Oslo
2018-2024
University of Bonn
2011-2018
Abstract The zebrafish embryo is a vertebrate well suited for visualizing nanoparticles at high resolution in live animals. Its optical transparency and genetic versatility allow noninvasive, real‐time observations of vascular flow their interactions with cells throughout the body. As consequence, this system enables acquisition quantitative data that are difficult to obtain rodents. Until now, few studies using model have only described semiquantitative results on key nanoparticle...
The constant exposure of the fish branchial cavity to aquatic pathogens causes local mucosal immune responses be extremely important for their survival. Here, we used a marker T lymphocytes/natural killer (NK) cells (ZAP70) and advanced imaging techniques investigate lymphoid architecture zebrafish cavity. We identified sub-pharyngeal organ, which tentatively named “Nemausean organ” (NELO). NELO is enriched in T/NK cells, plasma/B antigen-presenting embedded network reticulated epithelial...
Professional phagocytic cells such as macrophages are a central part of innate immune defence. They ingest microorganisms into membrane-bound compartments (phagosomes), which acidify and eventually fuse with lysosomes, exposing their contents to microbicidal environment. Gram-positive Rhodococcus equi can cause pneumonia in young foals immunocompromised humans. The possession virulence plasmid allows them subvert host defence mechanisms multiply macrophages. Here, we show that the...
Tuberculosis is the deadliest bacterial disease globally, threatening lives of millions every year. New antibiotic therapies that can shorten duration treatment, improve cure rates, and impede development drug resistance are desperately needed. Here, we used polymeric micelles to encapsulate four second-generation derivatives antitubercular pretomanid had previously displayed much better in vivo activity against Mycobacterium tuberculosis than itself. Because these compounds were relatively...
BackgroundThe developing zebrafish is an emerging tool in nanomedicine, allowing non-invasive live imaging of the whole animal at higher resolution than possible more commonly used mouse models. In addition, several transgenic fish lines are available endowed with selected cell types expressing fluorescent proteins; this allows nanoparticles to be visualized together host cells.MethodsHere, we introduce neural tube as a robust injection site for cancer cells, excellently suited high imaging....
The phylogenetic diversity of Ochrophyta, a diverse and ecologically important radiation algae, is still incompletely understood even at the level principal lineages. One taxon that has eluded simple classification marine flagellate genus Olisthodiscus . We investigated luteus K‐0444 documented its morphological genetic differences from NIES‐15 strain, which we described as tomasii sp. nov. Phylogenetic analyses combined 18S 28S rRNA sequences confirmed constitutes separate, deep, ochrophyte...
Abstract DNA-PAINT combined with total Internal Reflection Fluorescence (TIRF) microscopy enables the highest localization precisions, down to single nanometers in thin biological samples, due TIRF’s unique method for optical sectioning and attaining high contrast. However, most cellular targets elude accessible TIRF range close cover glass thus require alternative imaging conditions, affecting resolution image quality. Here, we address this limitation by applying ultrathin physical...
Rhodococcus equi is a close relative of Mycobacterium spp. and facultative intracellular pathogen which arrests phagosome maturation in macrophages before the late endocytic stage. We have screened transposon mutant library R. for mutants with decreased capability to prevent phagolysosome formation. This screen yielded gene β-ketoacyl-(acyl carrier protein)-synthase A (KasA), key enzyme long-chain mycolic acid synthesizing FAS-II system. The longest kasA chains were 10 carbon units shorter...
ABSTRACT Rhodococcus equi is an intracellular pathogen which causes pneumonia in young horses and immunocompromised humans. R. arrests phagosome maturation macrophages at a prephagolysosome stage grows inside privileged compartment. Here, we show that, murine activated with gamma interferon lipopolysaccharide, does not multiply but stays viable for least 24 h. Whereas infection control of other pathogens by executed enhanced acidification or phagolysosome formation, autophagy the...
The combination drug regimens that have long been used to treat tuberculosis (TB), caused by Mycobacterium tuberculosis, are fraught with problems such as frequent administration, duration of treatment, and harsh adverse effects, leading the emergence multidrug resistance. Moreover, there is no effective preventive vaccine against TB infection. In this context, nanoparticles (NPs) emerged a potential alternative method for delivery. Encapsulating antibiotics in biodegradable NPs has shown...
The cell wall of the pathogenic bacterium Rhodococcus equi (R. equi) contains abundant trehalose monomycolate (TMM) and dimycolate (TDM), glycolipids bearing mycolic acids. Here, we describe multiple-stage (MS(n)) linear ion-trap (LIT) mass spectrometric approaches toward structural characterization TMM TDM desorbed as [M + Alk](+) (Alk = Na, Li) X](-) (X CH(3)CO(2), HCO(2)) ions by electrospray ionization (ESI). Upon MS(n) (n=2, 3, 4) on or adduct TDM, structurally informative fragment are...
Members of the virulence-associated protein (Vap) family from pathogen Rhodococcus equi regulate virulence in an unknown manner. They do not share recognizable sequence homology with any known structure. VapB and VapA are normally associated isolates pigs horses, respectively. To contribute to a molecular understanding Vap function, crystal structure protease-resistant fragment was determined at 1.4 Å resolution. The solved by SAD phasing employing anomalous signal one endogenous S atom two...
ABSTRACT With the increasing emergence of drug-resistant Mycobacterium tuberculosis strains, new and effective antibiotics against (TB) are urgently needed. However, high frequency poorly water-soluble compounds among hits in high-throughput drug screening campaigns is a major obstacle discovery. Moreover, vivo testing using conventional animal TB models, such as mice, time consuming costly, represents bottleneck lead compound discovery development. Here, we report use zebrafish embryo model...
In the development of therapeutic nanoparticles (NP), there is a large gap between in vitro testing and vivo experimentation. Despite its prominence as model, mouse shows severe limitations for imaging NP cells with which they interact. Recently, transparent zebrafish larva, well suited high-resolution live-imaging, has emerged powerful alternative model to investigate behavior NP. Poly(D,L lactic acid) (PLA) widely accepted safe polymer prepare However, prevent aggregation, many require...
ABSTRACT The constant exposure of the fish branchial cavity to aquatic pathogens must have driven local mucosal immune responses be extremely important for their survival. In this study, we used a universal marker T lymphocytes/natural killer cells (ZAP70) and advanced imaging techniques investigate lymphoid architecture zebrafish cavity. We identified new organ, which tentatively named “Nemausean Lymphoid Organ” (NEMO), situated below pharynx, closely associated with gill tissues. Besides...
The unparalleled resolving power of electron microscopy is both a blessing and curse. At 30,000x magnification, 1 µm corresponds to 3 cm in the image field view only few micrometres or less, resulting an inevitable reduction spatial data available image. Consequently, gain resolution at cost loss contextual “reference space”, which crucial for understanding embedded structures interest. This problem particularly pronounced immuno-electron microscopy, where detection gold particle critical...
ABSTRACT Tuberculosis is the deadliest bacterial disease globally, threatening lives of millions every year. New antibiotic therapies that can shorten duration treatment, improve cure rates, and impede development drug resistance are desperately needed. Here, we used polymeric micelles to encapsulate four second-generation derivatives antitubercular pretomanid had previously displayed much better in vivo activity against Mycobacterium tuberculosis than itself. Because these compounds were...