A5058861052- Immune Response and Inflammation
- Escherichia coli research studies
- Vibrio bacteria research studies
- Streptococcal Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Phagocytosis and Immune Regulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Legionella and Acanthamoeba research
- Zoonotic diseases and public health
- Inflammasome and immune disorders
- Immune responses and vaccinations
- Amoebic Infections and Treatments
Imperial College London
2018-2019
Shigella flexneri is historically regarded as the primary agent of bacillary dysentery, yet closely-related sonnei replacing S. flexneri, especially in developing countries. The underlying reasons for this dramatic shift are mostly unknown. Using a zebrafish (Danio rerio) model infection, we discover that more virulent than vivo. Whole animal dual-RNAseq and testing bacterial mutants suggest virulence depends on its O-antigen oligosaccharide (which unique among species). We show vivo using...
Diarrheal disease remains the second leading cause of death in children under five. Shigella a significant diarrheal with two species, S. flexneri and sonnei , causing majority infections. are well known to cell macrophages, which contributes inflammatory nature diarrhea. Here, we demonstrate that causes less than due reduced number bacteria present cytosol. We identify O-Ag polysaccharide which, uniquely among spp., is forms on bacterial surface as factor responsible. Our data indicate...
Shigella flexneri and sonnei bacteria cause the majority of all shigellosis cases worldwide. However, their distributions differ, with S. predominating in middle- high-income countries low-income countries. One proposed explanation for continued range expansion is that it can survive amoebae, which could provide a protective environment bacteria. In this study, we demonstrate while both coculture free-living amoebae Acanthamoebae castellanii, bacterial growth predominantly extracellular. All...
Abstract Shigella flexneri is historically regarded as the primary agent of bacillary dysentery, yet closely-related sonnei replacing S. , especially in developing countries. The underlying reasons for this dramatic shift are mostly unknown. Using a zebrafish ( Danio rerio ) model infection, we discover that more virulent than vivo . Whole animal dual-RNAseq and testing bacterial mutants suggest virulence depends on its O-antigen oligosaccharide (which unique among species). We show using ex...
Abstract Two Shigella species, flexneri and sonnei , cause approximately 90% of bacterial dysentery worldwide. While S. is the dominant species in low-income countries, causes majority infections middle high-income countries. a prototypic cytosolic bacterium; once intracellular it rapidly escapes phagocytic vacuole pyroptosis macrophages, which important for pathogenesis spread. By contrast little known about invasion, escape induction during infection macrophages. We demonstrate that...