Mohammad Tahir Yousafzai
A5022663816- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- Child Nutrition and Water Access
- Vibrio bacteria research studies
- Vaccine Coverage and Hesitancy
- Respiratory viral infections research
- Hepatitis Viruses Studies and Epidemiology
- Viral Infections and Immunology Research
- Global Maternal and Child Health
- Clostridium difficile and Clostridium perfringens research
- Escherichia coli research studies
- SARS-CoV-2 and COVID-19 Research
- Liver Disease Diagnosis and Treatment
- Antibiotic Resistance in Bacteria
- Pneumonia and Respiratory Infections
- Hepatitis B Virus Studies
- Diabetes Management and Education
- COVID-19 epidemiological studies
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Hepatitis C virus research
- Gastrointestinal disorders and treatments
- Chronic Disease Management Strategies
- Cancer Research and Treatments
- Diabetes Management and Research
- Disaster Response and Management
Aga Khan University
2016-2025
UNSW Sydney
2020-2025
Aga Khan University Hospital
2017-2024
Aga Khan University
2013-2023
Kenya Medical Research Institute
2023
Hamad Medical Corporation
2012-2014
Weill Cornell Medical College in Qatar
2012-2014
Baqai Medical University
2013-2014
Jinnah Medical & Dental College
2013
Cornell University
2012
Antibiotic resistance is a major problem in Salmonella enterica serovar Typhi, the causative agent of typhoid. Multidrug-resistant (MDR) isolates are prevalent parts Asia and Africa often associated with dominant H58 haplotype. Reduced susceptibility to fluoroquinolones also widespread, sporadic cases third-generation cephalosporins or azithromycin have been reported. Here, we report first large-scale emergence spread novel S Typhi clone harboring three first-line drugs (chloramphenicol,...
The aim was to compare body mass index (BMI), waist circumference (WC), hip ratio (WHR), and height (WHtR) identify the best predictor of metabolic syndrome (MetS) among Qatari adult population. A cross-sectional survey from April 2011 December 2012. Data collected 1552 participants followed by blood sampling. MetS defined according Third Adult Treatment Panel (ATPIII) International Diabetes Federation (IDF). Receiver operating characteristics (ROC) curve analysis performed. Among men, WC...
Salmonella enterica serotype Typhi (S Typhi) is a major public health problem in low-income and middle-income countries. We aimed to investigate the effectiveness impact of typhoid conjugate vaccine Typbar-TCV against S among children an outbreak setting extensively drug-resistant (XDR) Pakistan.This cohort study was done from Feb 21, 2018, Dec 31, 2019. A census survey all households located Qasimabad Latifabad subdistricts Hyderabad, Pakistan, at baseline, 174 005 were registered census....
Precise enteric fever disease burden data are needed to inform prevention and control measures, including the use of newly available typhoid vaccines. We established Surveillance for Enteric Fever in Asia Project (SEAP) these strategies.
BackgroundThe incidence of enteric fever, an invasive bacterial infection caused by typhoidal Salmonellae (Salmonella enterica serovars Typhi and Paratyphi), is largely unknown in regions without blood culture surveillance. The aim this study was to evaluate whether new diagnostic serological markers for Salmonella can reliably estimate population-level incidence.MethodsWe collected longitudinal samples from patients with culture-confirmed fever enrolled surveillance studies Bangladesh,...
Abstract Background Shigella is a leading cause of acute watery diarrhea, dysentery, and diarrhea-attributed linear growth faltering, precursor to stunting lifelong morbidity. Several promising vaccines are in development field efficacy trials will require consortium potential vaccine trial sites with up-to-date diarrhea incidence data. Methods The Enterics for Global Health (EFGH) surveillance study employ facility-based enrollment cases aged 6–35 months 3 follow-up establish rates document...
Shigella is a major cause of diarrhea in young children worldwide. Multiple vaccines targeting are development, and phase 3 clinical trials imminent to determine efficacy against shigellosis. The Enterics for Global Health (EFGH) surveillance study designed the incidence medically attended shigellosis 6- 35-month-old 7 resource-limited settings. Here, we describe microbiological methods used isolate identify Shigella. We developed standardized laboratory protocol isolation identification by...
Quantitative polymerase chain reaction (qPCR) targeting ipaH has been proven to be highly efficient in detecting Shigella clinical samples compared culture-based methods, which underestimate burden by 2- 3-fold. qPCR assays have also developed for speciation and serotyping, is critical both vaccine development evaluation. The Enterics Global Health (EFGH) surveillance study will utilize a customized real-time PCR-based TaqMan Array Card (TAC) interrogating 82 targets, the detection...
The Aga Khan University clinical microbiology laboratory identified an outbreak of ceftriaxone-resistant Salmonella Typhi in Hyderabad, Pakistan, through antimicrobial resistance surveillance. An investigation was carried out to identify the risk factors and institute control measures. Here we report preliminary findings this investigation, using data collected from 30 November 2016 28 March 2017. design for a case-control study that included identification culture-proven S. cases, suspected...
Abstract Background Clinicians have limited therapeutic options for enteric as a result of increasing antimicrobial resistance, and therefore typhoid vaccination is recommended preventive measure. As part the Surveillance Enteric Fever in Asia Project (SEAP), we investigated extent measured burden resistance (AMR) among confirmed fever cases Bangladesh, Nepal, Pakistan. Methods From September 2016–September 2019, SEAP recruited study participants all age groups from its outpatient,...
The objective of Phase I the Surveillance for Enteric Fever in Asia Project (SEAP), a multiphase surveillance study characterizing burden disease South Asia, was to inform data collection prospective and capture clinical aspects disease.Through retrospective record review conducted at hospitals Bangladesh, India, Nepal, Pakistan, we examined laboratory records assess culture positivity rate Salmonella Typhi Paratyphi, age sex distribution, antimicrobial susceptability each country.Of all...
Introduction Salmonella Typhi is one of the leading health problems in Pakistan. With emergence extensively drug resistant (XDR) Typhi, treatment options are limited. Here we report clinical manifestations and response to patients with XDR Typhoid fever. The were treated either Meropenem or Azithromycin a combination both. Methods We reviewed records culture confirmed typhoid who visited Aga Khan University Hospital (AKUH), Karachi Secondary Care Hospital, Hyderabad from April 2017 June...
The Surveillance for Enteric Fever in Asia Project (SEAP) is a multisite surveillance study designed to capture morbidity and mortality burden of enteric fever (typhoid paratyphoid) Bangladesh, Nepal, Pakistan. We aim describe disease burden, severity illness, antimicrobial resistance trends Pakistan.In this retrospective, cross-sectional study, laboratory records hospitalized patients who received blood culture any 3 Aga Khan University hospitals Karachi Hyderabad, Pakistan, from 2012 2014...
Abstract Background Bacterial pathogens cause substantial diarrhea morbidity and mortality among children living in endemic settings, yet antimicrobial treatment is only recommended for dysentery or suspected cholera. Methods AntiBiotics Children with severe Diarrhea was a 7-country, placebo-controlled, double-blind efficacy trial of azithromycin 2–23 months age watery accompanied by dehydration malnutrition. We tested fecal samples enteric utilizing quantitative polymerase chain reaction to...
Rigorous data management systems and planning are essential to successful research projects, especially for large, multicountry consortium studies involving partnerships across multiple institutions. Here we describe the development implementation of procedures Enterics For Global Health (EFGH) Shigella surveillance study-a 7-country diarrhea study that will conduct facility-based concurrent with population-based enumeration a health care utilization survey estimate incidence...
Accurate estimation of diarrhea incidence from facility-based surveillance requires estimating the population at risk and accounting for case patients who do not seek care. The Enterics Global Health (EFGH) Shigella study will characterize denominators healthcare-seeking behavior proportions to calculate rates in children aged 6-35 months across 7 sites Africa, Asia, Latin America. use a hybrid design, supplementing with population-based surveys estimate size proportion brought care EFGH...
Abstract Background The Enterics for Global Health (EFGH) Shigella surveillance study is a longitudinal multicountry that aims to estimate incidence rates and document consequences of diarrhea within 7 countries in Africa, Asia, Latin America. In addition high childhood diarrhea, Pakistan facing problem antimicrobial resistance urban peri-urban areas Karachi. Methods Pakistan, EFGH will be conducted Karachi, which one the metropolitan cities bordering Arabian Sea has diverse population 1.6...
Abstract Background Typhoid fever burdens low- and middle-income countries, especially children. Despite being curable, it now resists first-line antibiotics. This study aims to understand antimicrobial resistance patterns associated with multi-drug resistant (MDR) extensively drug (XDR) typhoid cases in Pakistan. Methods We conducted a retrospective review of blood culture confirmed from five large laboratory networks Pakistan over period 3 years (2017-2019). Data was analyzed for 464,956...