A5007177485- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Pneumonia and Respiratory Infections
- Salmonella and Campylobacter epidemiology
- S100 Proteins and Annexins
- Inflammation biomarkers and pathways
- Viral gastroenteritis research and epidemiology
- Antibiotic Resistance in Bacteria
- Genetics, Bioinformatics, and Biomedical Research
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Hematological disorders and diagnostics
- Biomedical Text Mining and Ontologies
MRC Unit the Gambia
2016-2024
Quantitative polymerase chain reaction (qPCR) targeting ipaH has been proven to be highly efficient in detecting Shigella clinical samples compared culture-based methods, which underestimate burden by 2- 3-fold. qPCR assays have also developed for speciation and serotyping, is critical both vaccine development evaluation. The Enterics Global Health (EFGH) surveillance study will utilize a customized real-time PCR-based TaqMan Array Card (TAC) interrogating 82 targets, the detection...
Abstract Background Most tuberculosis (TB) cases in The Gambia are notified the Greater Banjul Area (GBA). We conducted an Enhanced-Case-Finding (ECF) intervention GBA and determined its effect on TB incidence ongoing transmission. Methods This was a cluster randomized trial which randomly assigned areas of grouped settlements received three rounds ECF strategy consisting sensitization followed by auramine microscopy, whereas patients control continued to be identified through passive case...
The geographically restricted Mycobacterium africanum lineages (MAF) are primarily found in West Africa, where they account for a significant proportion of tuberculosis. Despite this phenomenon, little is known about the co-evolution these ancient with Africans. MAF and M. tuberculosis sensu stricto (MTB) differ their clinical, vitro vivo characteristics reasons not fully understood. Therefore, we compared genomes 289 205 MTB clinical isolates from 6 main human-adapted complex lineages,...
Background Mycobacterium tuberculosis culturing remains the gold standard for laboratory diagnosis of tuberculosis. Tuberculosis a great public health problem in developing countries like The Gambia, as most methods currently used bacterial isolation are either time-consuming or costly. Objective To evaluate Kudoh swab method West African setting with particular focus on method’s performance when africanum Africa 2 (MAF2) isolates. Method 75 sputum samples were collected Greater Banjul Area...
To determine the culture yield and time to detection of mycobacterial growth between samples decontaminated using 0.7% chlorhexidine sodium hydroxide–N-acetyl-l-cysteine (NaOH–NALC) cultured on Löwenstein–Jensen (LJ) medium. We also aimed contamination rate NaOH–NALC decontamination methods. The study was carried out 68 sputa (42 smear positives 26 negatives). Of these samples, 46 were collected from men women with an approximate average age 27 years. All sputum standard concentrates in...
To evaluate the Kudoh swab method for improving laboratory diagnosis of tuberculosis (TB) in Gambia.A total 75 sputa (50 smear positive and 25 negative) were examined. Sputum samples collected from leftover routine Medical Research Council Unit, Gambia TB Diagnostic Laboratory. The processed using standard N-acetyl-l-cysteine-NaOH (NALC-NaOH) methods currently used method. These cultured on Lowenstein Jensen (LJ) Modified Ogawa media, respectively, incubated aerobically at 36±1°C...
Summary The geographically restricted Mycobacterium africanum lineages (MAF) are primarily found in West Africa, where they account for a significant proportion of tuberculosis. Despite this phenomenon, little is known about the co-evolution these ancient with Africans. MAF and M. tuberculosis sensu stricto (MTB) differ their clinical, vitro vivo characteristics reasons not fully understood. Therefore, we compared genomes 289 205 MTB clinical isolates from 6 main human-adapted complex...
<h3>Background</h3> Tuberculosis is a leading infectious disease killer. It primarily affects the lung, accompanied by tissue damage from excessive host inflammation. Neutrophils are implicated as primary mediators of this destruction. This study aims to access neutrophils and their soluble in relation TB-induced damage. <h3>Methods</h3> Fifty-three (53) patients with confirmed TB were recruited. Neutrophil numbers sputum blood assessed using microscopy automated counting. Soluble analysed...