A5033438385- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Diagnosis and treatment of tuberculosis
- Antibiotic Resistance in Bacteria
- Infectious Diseases and Tuberculosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Pneumonia and Respiratory Infections
- Infectious Diseases and Mycology
- Biochemical and Molecular Research
- Genomics and Phylogenetic Studies
- HIV/AIDS drug development and treatment
- Cancer therapeutics and mechanisms
- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Biosimilars and Bioanalytical Methods
- vaccines and immunoinformatics approaches
- RNA and protein synthesis mechanisms
- Leprosy Research and Treatment
- Microbial Community Ecology and Physiology
- Animal Disease Management and Epidemiology
- Quinazolinone synthesis and applications
- Ginseng Biological Effects and Applications
- Cell Image Analysis Techniques
- Brucella: diagnosis, epidemiology, treatment
- Health Policy Implementation Science
Instituut voor Tropische Geneeskunde
2016-2025
University of Antwerp
2016-2025
Institut Pasteur de Lille
2005-2024
Université Paris Cité
2024
Université Bourgogne Franche-Comté
2024
London School of Hygiene & Tropical Medicine
2024
Université Paris-Saclay
2024
Center for Infection and Immunity of Lille
2024
AgroParisTech
2024
Research Institute for Tropical Medicine
2021
Abstract Background The Direct Repeat locus of the Mycobacterium tuberculosis complex (MTC) is a member CRISPR (Clustered regularly interspaced short palindromic repeats) sequences family. Spoligotyping widely used PCR-based reverse-hybridization blotting technique that assays genetic diversity this and useful both for clinical laboratory, molecular epidemiology, evolutionary population genetics. It easy, robust, cheap, produces highly diverse portable numerical results, as result...
A clear understanding of the genetic basis antibiotic resistance in Mycobacterium tuberculosis is required to accelerate development rapid drug susceptibility testing methods based on sequence. Raw genotype–phenotype correlation data were extracted as part a comprehensive systematic review develop standardised analytical approach for interpreting associated mutations rifampicin, isoniazid, ofloxacin/levofloxacin, moxifloxacin, amikacin, kanamycin, capreomycin, streptomycin,...
The rapid diagnosis of rifampin resistance is hampered by a reported insufficient specificity molecular techniques for detection rpoB mutations. Our objective this study was to document the prevalence and prognostic value mutations with unclear phenotypic resistance. design entailed sequencing directly from sputum first failure or relapse patients without selection comparison standard retreatment regimen outcome, according mutation present. We found that among all mutations, best-documented...
Abstract The human- and animal-adapted lineages of the Mycobacterium tuberculosis complex (MTBC) are thought to have expanded from a common progenitor in Africa. However, molecular events that accompanied this emergence remain largely unknown. Here, we describe two MTBC strains isolated patients with multidrug resistant tuberculosis, representing an as-yet-unknown lineage, named Lineage 8 (L8), seemingly restricted African Great Lakes region. Using genome-based phylogenetic reconstruction,...
Abstract Drug resistance diagnostics that rely on the detection of resistance-related mutations could expedite patient care and TB eradication. We perform minimum inhibitory concentration testing for 12 anti-TB drugs together with Illumina whole-genome sequencing 1452 clinical Mycobacterium tuberculosis (MTB) isolates. evaluate genome-wide associations between in MTB genes or non-coding regions resistance, followed by validation an independent data set 792 confirm at 13 non-canonical loci,...
WHO-endorsed phenotypic drug susceptibility testing (DST) methods for Mycobacterium tuberculosis are assumed to be the gold standard identifying rifampin (RMP) resistance. However, previous results indicated that low-level, yet probably clinically relevant, RMP resistance linked specific rpoB mutations is easily missed by some growth-based methods. We aimed compare level of detected on Löwenstein-Jensen (LJ) medium with Bactec MGIT 960 automated DST (MGIT-DST) system various mutants. Full...
ABSTRACT The objectives of this study were to investigate the origin highly discordant rifampin (rifampicin) (RMP) drug susceptibility test results obtained for Mycobacterium tuberculosis strains during proficiency testing. Nine Supra-National Tuberculosis Reference Laboratories tested RMP susceptibilities 19 selected M. strains, using standard culture-based methods. classified as definitely resistant (R) ( n = 6) or susceptible (S) 2) probably (PR) 8) (PS) 3) based on rpoB mutations and...
ABSTRACT The aminoglycosides kanamycin and amikacin the macrocyclic peptide capreomycin are key drugs for treatment of multidrug-resistant tuberculosis (MDR-TB). increasing rates resistance to these possible cross-resistance between them concerns MDR-TB therapy. Mutations in 16S rRNA gene ( rrs ) have been associated with each drugs, mutations tlyA gene, which encodes a putative methyltransferase, thought confer Mycobacterium bacteria. Studies shown variable results. In this study, MICs 145...
BackgroundPyrazinamide and fluoroquinolones are essential antituberculosis drugs in new rifampicin-sparing regimens. However, little information about the extent of resistance to these at population level is available.MethodsIn a molecular epidemiology analysis, we used population-based surveys from Azerbaijan, Bangladesh, Belarus, Pakistan, South Africa investigate pyrazinamide among patients with tuberculosis. Resistance was assessed by gene sequencing detection resistance-conferring...
Resistance-associated variants (RAVs) in Rv0678 , a regulator of the MmpS5-MmpL5 efflux pump, have been shown to lead increased MICs bedaquiline (2- 8- fold) and clofazimine 4-fold). The prevalence these RAVs clinical isolates their impact on treatment outcomes are important factors take into account guidelines.Baseline from two MDR-TB trials were sequenced for corresponding determined 7H11 agar. also investigated non-MDR-TB sequences population-based cohort.Rv0678 identified 23/347 (6.3%)...
In many countries, regular monitoring of the emergence resistance to anti-tuberculosis drugs is hampered by limitations phenotypic testing for drug susceptibility. We therefore evaluated use genetic sequencing surveillance in tuberculosis.Population-level surveys were done hospitals and clinics seven countries (Azerbaijan, Bangladesh, Belarus, Pakistan, Philippines, South Africa, Ukraine) evaluate estimate Mycobacterium tuberculosis isolates rifampicin, isoniazid, ofloxacin, moxifloxacin,...
The development of molecular diagnostics that detect both the presence Mycobacterium tuberculosis in clinical samples and drug resistance-conferring mutations promises to revolutionize patient care interrupt transmission by ensuring early diagnosis. However, these tools require identification genetic determinants resistance full range antituberculosis drugs.
Abstract The majority of Mycobacterium tuberculosis isolates resistant to isoniazid harbour a mutation in katG . Since these mutations cause wide range minimum inhibitory concentrations (MICs), largely below the serum level reached with higher dosing (15 mg/L upon 15–20 mg/kg), drug might still remain partly active presence mutation. We therefore investigated which genetic predict phenotypic resistance clinical M. isolates. To this end, association between known and unknown...
Combating the spread of drug resistant tuberculosis is a global health priority. Whole genome association studies are being applied to identify genetic determinants resistance anti-tuberculosis drugs. Protein structure and interaction modelling used understand functional effects putative mutations provide insight into molecular mechanisms leading resistance.To investigate potential utility these approaches, we analysed genomes 144 Mycobacterium clinical isolates from The Special Programme...
Background In light of the limitations current case finding strategies and global urgency to improve tuberculosis (TB) case-detection, a renewed interest in active (ACF) has risen. The WHO calls for more evidence on innovative ways TB screening, especially from low-income countries, inform guideline development. We aimed assess feasibility community-based ACF among urban poor Cambodia determine its impact detection, treatment uptake outcome. Methods Between 9/2/2012-31/3/2013 Sihanouk...
Greater Mymensingh area, Bangladesh.To document among new tuberculosis (TB) patients the proportions and treatment outcomes of silent, non-disputed disputed (generally missed by rapid drug susceptibility testing [DST]) rpoB mutations, their detection commercial molecular assays.Retrospective analysis sequences from randomly selected ethanol-preserved diagnostic sputum samples; comparison sequencing with conventional DST results standard first-line outcome; retesting samples mutations using...
Drug-resistant tuberculosis poses a persistent public health threat. The ReSeqTB platform is collaborative, curated knowledgebase, designed to standardize and aggregate global Mycobacterium complex (MTBC) variant data from whole genome sequencing (WGS) with phenotypic drug susceptibility testing (DST) clinical data. We developed unified analysis pipeline (UVP) ( https://github.com/CPTR-ReSeqTB/UVP ) identify variants assign lineage MTBC sequence Stringent thresholds quality control measures...
Conventional molecular tests for detecting Mycobacterium tuberculosis complex (MTBC) drug resistance on clinical samples cover a limited set of mutations. Whole-genome sequencing (WGS) typically requires culture. Here, we evaluated the Deeplex Myc-TB targeted deep-sequencing assay prediction to 13 anti-tuberculous drugs/drug classes, directly applicable sputum. With MTBC DNA tests, limit detection was 100–1000 genome copies fixed captured in silico 97.1–99.3% phenotypes correctly predicted...
The definition of a genus has wide-ranging implications both in terms binomial species names and also evolutionary relationships. In recent years, the
Bedaquiline is a life-saving tuberculosis drug undergoing global scale-up. People at risk of weak regimens are priority for novel access despite the potential source Mycobacterium tuberculosis-resistant strains. We aimed to characterise bedaquiline resistance in individuals who had sustained culture positivity during bedaquiline-based treatment.
Abstract Analysis of genome sequencing data from >100,000 genomes Mycobacterium tuberculosis complex using TB-Annotator software revealed a previously unknown lineage, proposed name L10, in central Africa. Phylogenetic reconstruction suggests L10 could represent missing link the evolutionary and geographic migration histories M. africanum.
Studies on recurrent tuberculosis (TB), TB molecular epidemiology and drug susceptibility testing rely the analysis of one Mycobacterium isolate from a single sputum sample collected at different disease episodes. This scheme rests postulate that culture is homogeneous representative total bacillary population in patient. We systematically analysed several pre-treatment isolates each 199 smear-positive male adult inmates admitted to prison hospital by standard IS6110 DNA fingerprinting,...
We have identified a clonal complex of Mycobacterium bovis present at high frequency in cattle population samples from several sub-Saharan west-central African countries. This closely related group bacteria is defined by specific chromosomal deletion (RDAf1) and can be the absence spacer 30 standard spoligotype typing scheme. named this strains 1 (Af1) signature as being same M. BCG vaccine strain but with 30. Strains Af1 were found Mali, Cameroon, Nigeria, Chad, using combination...