A5075651256- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Antibiotic Resistance in Bacteria
- Pneumonia and Respiratory Infections
- Pneumocystis jirovecii pneumonia detection and treatment
- Cancer therapeutics and mechanisms
- Advanced biosensing and bioanalysis techniques
- Biotin and Related Studies
- Antimicrobial Resistance in Staphylococcus
- Pharmaceutical and Antibiotic Environmental Impacts
- Molecular Junctions and Nanostructures
- Antibiotics Pharmacokinetics and Efficacy
- Salmonella and Campylobacter epidemiology
- Animal Disease Management and Epidemiology
- Influenza Virus Research Studies
- Viral Infections and Vectors
Sciensano (Belgium)
2023-2024
Rega Institute for Medical Research
2023
KU Leuven
2023
Université Libre de Bruxelles
2023
Royal Meteorological Institute of Belgium
2023
Instituut voor Tropische Geneeskunde
2016-2019
BackgroundPyrazinamide and fluoroquinolones are essential antituberculosis drugs in new rifampicin-sparing regimens. However, little information about the extent of resistance to these at population level is available.MethodsIn a molecular epidemiology analysis, we used population-based surveys from Azerbaijan, Bangladesh, Belarus, Pakistan, South Africa investigate pyrazinamide among patients with tuberculosis. Resistance was assessed by gene sequencing detection resistance-conferring...
In many countries, regular monitoring of the emergence resistance to anti-tuberculosis drugs is hampered by limitations phenotypic testing for drug susceptibility. We therefore evaluated use genetic sequencing surveillance in tuberculosis.Population-level surveys were done hospitals and clinics seven countries (Azerbaijan, Bangladesh, Belarus, Pakistan, Philippines, South Africa, Ukraine) evaluate estimate Mycobacterium tuberculosis isolates rifampicin, isoniazid, ofloxacin, moxifloxacin,...
Abstract We compared the ability of commercial and non-commercial, phenotypic genotypic rapid drug susceptibility tests (DSTs) to detect rifampicin resistance (RR)-conferring ‘disputed’ mutations frequently missed by Mycobacterium Growth Indicator Tube (MGIT), namely L430P, D435Y, L452P, I491F. Strains with mutation S450L served as positive control while wild-types were used negative control. Of 38 mutant strains, 5.7% classified RR MGIT, 16.2% Trek Sensititre MYCOTB MIC plate, 19.4%...
Biotinylation is widely used in DNA, RNA and protein probing assays as this molecule has generally no impact on the biological activity of its substrate. During streptavidin-based detection glycoproteins Lactobacillus rhamnosus GG with biotinylated lectin probes, a strong positive band approximately 125 kDa was observed, present different cellular fractions. This potential glycoprotein reacted heavily concanavalin A (ConA), that specifically binds glucose mannose residues. Surprisingly,...
The high economic impact and zoonotic potential of avian influenza call for detailed investigations dispersal dynamics epidemics. We integrated phylogeographic epidemiologic analyses to investigate the a low pathogenicity (H3N1) epidemic that occurred in Belgium during 2019. Virus genomes from 104 clinical samples originating 85% affected farms were sequenced. A spatially explicit analysis confirmed dominating northeast southwest direction long-distance event linked direct live animal...
Mycobacterium africanum lineage (L) 6 is an important pathogen in West Africa, causing up to 40% of pulmonary tuberculosis (TB). The biology underlying the clinical differences between M. and sensu stricto remains poorly understood. We performed ex vivo expression 2179 genes most geographically dispersed cause human TB, L4 restricted, L6 directly from sputa 11 HIV-negative TB patients Gambia who had not started treatment. DosR regulon was significantly decreased category relative L4....
The development of rapid molecular diagnostic assays for pyrazinamide (PZA) resistance is considered technically challenging as mutations are highly diverse, scattered along the full length pncA gene and not all associated with PZA resistance. We evaluated performance novel Genoscholar PZA-TB II line probe assay (PZA-LPA2; NIPRO Corporation, Japan).To evaluate applicability PZA-LPA2 in clinical settings, we compared to a composite reference standard Sanger Illumina sequencing plus phenotypic...
Linezolid is a critically important antimicrobial used in human medicine. While linezolid not licensed for food-producing animals, the veterinary use of other antimicrobials, such as phenicols (e.g., florfenicol), could cross/co-select linezolid-resistant (LR) bacteria. Such LR strains pose great concern public health due to their potential transfer between animals and humans. This study explored possible associations epidemiological risk factors, including phenicol use, occurrence bacteria,...
Abstract Background: Fluoroquinolones (FQs) have substantial activity against the Mycobacterium tuberculosis complex (MTBc) by preventing bacterial DNA synthesis through gyrase inhibition. The reference standard for FQ-resistance testing is phenotypic drug-susceptibility (pDST) based on growth inhibition of MTBc in drug-containing Mycobacteria Growth Indicator Tube system (MGIT) media at a critical concentration (CC) that differentiates phenotypically wild-type from nonwild-type and clinical...
In this work, we validated raw results of the new version Xpert MTB/RIF, Ultra, and found that unique combinations Ultra probe melting temperature shift (ΔTm) can discriminate between rifampicin-resistance (RR) conferring rpoB mutations identify specific RR including disputed commonly missed in rapid phenotypic drug susceptibility tests. Negative ΔTm was associated with all except those codon 450. The combination values capturing probes enabled to differentiate codons 428, 430, 431, 432,...