A5068235254- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Virus-based gene therapy research
- Microbial infections and disease research
- Leptospirosis research and findings
- Animal Disease Management and Epidemiology
- Veterinary medicine and infectious diseases
- Diagnosis and treatment of tuberculosis
- Infectious Diseases and Tuberculosis
- Viral Infections and Immunology Research
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Plant Molecular Biology Research
- Viral Infections and Vectors
- Insect and Pesticide Research
- Plant Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Plant nutrient uptake and metabolism
- Vector-Borne Animal Diseases
- Hepatitis Viruses Studies and Epidemiology
- Herpesvirus Infections and Treatments
- Plant and animal studies
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"
2016-2025
University of Padua
2024
Warsaw University of Life Sciences
2024
University of Turin
2020
Istituto Superiore di Sanità
2016-2020
University of Bari Aldo Moro
2017
Ministry of Agriculture and Rural Development
2016
University of Minnesota
2015
Armauer Hansen Research Institute
2012
Makerere University
2012
ABSTRACT Deformed wing virus (DWV) of honeybees ( Apis mellifera ) is closely associated with characteristic deformities, abdominal bloating, paralysis, and rapid mortality emerging adult bees. The was purified from diseased insects, its genome cloned sequenced. genomic RNA DWV 10,140 nucleotides in length contains a single large open reading frame encoding 328-kDa polyprotein. coding sequence flanked by 1,144-nucleotide 5′ nontranslated leader 317-nucleotide 3′ region, followed poly(A)...
Tuberculosis (TB) affects humans and other animals is caused by bacteria from the Mycobacterium tuberculosis complex (MTBC). Previous studies have shown that there are at least nine members of MTBC infecting than humans; these also been referred to as ecotypes. However, ecology evolution animal-adapted ecotypes poorly understood. Here we screened 12,886 publicly available genomes newly sequenced 17 strains, gathering a total 529 strains. Phylogenomic comparative analyses confirm paraphyletic...
Selective ubiquitin-mediated proteolysis through the cell cycle controls availability, and therefore activity, of several proliferation proteins. E2F transcription factors play distinct roles in both proliferating differentiated cells by regulating gene expression. Here, we report that Arabidopsis AtE2Fc is regulated a balance between expression ubiquitin-proteasome proteolysis. degradation implicates function E3 ubiquitin-ligase Skp1, Cullin, F-box (SCFAtSKP2) complex seems to be dependent...
Abstract Porcine epidemic diarrhea virus (PEDV) has been detected sporadically in Italy since the 1990s. We report phylogenetic relationship of swine enteric coronaviruses collected during 2007–2014 and identify a drastic shift PEDV strain variability new coronavirus generated by recombination transmissible gastroenteritis PEDV.
Bats are natural reservoirs for many mammalian coronaviruses, which have received renewed interest after the discovery of severe acute respiratory syndrome (SARS) and Middle East (MERS) CoV in humans. This study describes identification molecular characterization alphacoronaviruses betacoronaviruses bats Italy, from 2010 to 2012. Sixty-nine faecal samples 126 carcasses were tested using pan-coronavirus RT-PCR. Coronavirus RNAs detected seven nine carcasses. A phylogenetic analysis...
Leptospirosis is an infectious disease widely reported in veterinary practice and a worldwide zoonosis. In Northeastern Italy, different serogroups genotypes of Leptospira have been described ill dogs, the most commonly detected being Icterohaemorragiae (ICT) ST 17, Australis (AUS) 24 198, Pomona (POM) 117 289, Sejroe (SEJ) 155. However, there little information available on environmental exposure to wild synanthropic animals. The aim this study was identify circulating potential reservoirs...
Successful reproductive performance is key to farm competitiveness in the global marketplace. Porcine parvovirus 1 (PPV1) has been identified as a major cause of failure, and since 2001 new species porcine parvoviruses, namely PPV2–7, have identified, although their role not yet fully understood yet. The present study aimed investigate PPVs’ presence failure outbreaks occurring 124 farms northern Italy. Fetuses were collected from 338 sows between 2019 2021 tested for PPVs by real-time...
In eukaryotic cells, the function of DNA replication licensing components (Cdc6 and Cdt1, among others) is crucial for cell proliferation genome stability. However, little known about their role in whole organisms whether control interfaces with differentiation developmental programs. Here, we study Arabidopsis thaliana CDT1, its regulation, consequences overriding control. The availability AtCDT1 strictly regulated at two levels: (1) transcription level, by E2F growth-arresting signals, (2)...
Summary The activity of cyclin‐dependent kinases (CDK) is crucial for cell‐cycle transitions. Here, we report the identification a CDK that phosphorylates retinoblastoma‐related (RBR) protein. A CDK/cyclin complex binds to and RBR may be isolated from various plant sources, e.g. wheat, maize, Arabidopsis thaliana tobacco, cells growing under conditions. presence an RBR‐associated correlates with proliferative activity, suggesting phosphorylation major event in actively proliferating tissues....
ABSTRACT We have identified a clonal complex of Mycobacterium bovis isolated at high frequency from cattle in Uganda, Burundi, Tanzania, and Ethiopia. named this related group M. strains the African 2 (Af2) bovis. Af2 are defined by specific chromosomal deletion (RDAf2) can be absence spacers 3 to 7 their spoligotype patterns. Deletion analysis isolates Algeria, Mali, Chad, Nigeria, Cameroon, South Africa, Mozambique did not identify any complex, suggesting that is localized East Africa. The...
Spoligotyping and exact tandem repeat (ETR) analysis of Mycobacterium bovis M. caprae isolated strains has been routinely carried out in Italy since 2000 to obtain a database genetic profiles support traditional epidemiological investigations. In this study, we characterized 1,503 57 isolates obtained from 2006 747 cattle herds mainly located northern Italy. We identified 81 spoligotypes 113 ETR profiles, while the combination spoligotyping/ETR differentiated 228 genotypes, with genotypic...
Bovine tuberculosis (bTB) is an emerging disease among wild animals in many parts of the world. Wildlife reservoir hosts may thus represent a potential source infection for livestock and humans. We investigated role played by Sicilian black pig, autochthonous free- or semi-free-ranging domestic pig breed, as bTB area where prevalence cattle high. initially performed preliminary field study to assess occurrence such animals. sampled 119 pigs at abattoir found 6.7% 3.4% them be affected gross...
The demand for rapid methods the quantification of pathogens is increasing. Among these methods, those based on nucleic acids amplification (quantitative PCRs) are most widespread worldwide. Together with qPCR, a new approach named digital PCR (dPCR), has rapidly gained importance. aim our study was to compare results obtained using two different dPCR systems and one qPCR in three bacterial pathogens: Listeria monocytogenes, Francisella tularensis, Mycobacterium avium subsp....
Association of the retinoblastoma (Rb) protein with E2F transcription factors is central to cell cycle-specific gene expression and growth in animal cells. Whether Rb-E2F complexes are also involved plant differentiation still unknown since proteins have not yet been identified plants. Here we report isolation characterisation a wheat (TmE2F) cDNA clone. Interestingly, overall domain organisation related human E2F-1/2/3 subset but its primary sequence slightly more E2F-4/5 subset. TmE2F-Rb...
Summary This study describes the isolation and molecular characterization of Mammalian orthoreovirus (MRV) in microbats. Faecal samples dead individuals available from rehabilitation centres or collected known roost sites were virologically tested. In total, 112 carcasses bats found dead, 44 faecal analysed. Nineteen viral strains isolated by vitro cell culture tissue different bat species (Pipistrellus khulii, Tadarida teniotis, Rhinolophus hipposideros Vespertilio murinus), they...
Leptospirosis in dogs has been largely described worldwide, and epidemiological studies have mainly based on serological data. This study aims to detect genotype leptospires affecting symptomatic Northeast Italy between 2013 2019. Overall, 1631 were tested using real-time PCR, from 193 subjected Multilocus Sequence Typing a Multiple Loci Variable-number Tandem Repeat Analysis. Leptospires successfully isolated 15 dogs. Six distinct Types (STs) found for 135 leptospires, with 3 STs...